Figure 5.

The influence of 48 h of exposure of T. pratense extracts on the migration (A) and wound healing (B) of MDA-MB-231 cells. Migration rates of MDA-MB-231 cells incubated with extracts at IC₀ dosages into the free detection zones were photographed (×8) (C). Cell adhesion to the substrate was measured after staining with crystal violet; randomly chosen fields were photographed at ×200 (D). Control cells were not exposed to any compound but the vehicle; values are means ± standard deviations from at least three independent experiments; statistical significance was calculated versus control cells (untreated); ** p ≤ 0.01, *** p ≤ 0.001.