Video 7. Pavarotti phosphorylation at Ser745 is required to inhibit sliding.
Timelapse imaging of photoconverted microtubules in Drosophila S2 cells under control conditions or with depletion of Pavarotti using dsRNA against a non-coding region. Expression of WT Pav can rescue the effect of Pav RNAi. Expression of Phopsho-null Pavarotti is unable to rescue the effect of Pav RNAi. Microtubules were labeled with tdEOS alpha Tubulin 84b. 1 frame per minute.