Table 2.
Primer pairs used in this study.
| Target Name | Primer | Sequence (5′-3′) 1 | Tm (°C) | PCR Product Size (bp) | Efficiency (E) |
|---|---|---|---|---|---|
| alpha-acetolactate decarboxylase (ald) | Ald_F | TTCGAAGCCAAGAATATGSCC | 55 | 156 | 0.93 |
| Ald_R | GCYTCRGGTCAAAAAGAATT | ||||
| aspartate aminotransferase (ast) | Ast_F | CAATAACTRATMCGCGCATG | 50 | 143 | 1.13 |
| Ast_R | TTCCCGAAYATYAAGCG | ||||
| citrate lyase (subunit alpha) (cly) | Cly_F | GACAGGTTYTTGATYCCCATT | 55 | 148 | 1.22 |
| Cly_R | AACTMGTGGCGTCAATTCA | ||||
| 16S rRNA | TBA_FW 2 | CGGCAACGAGCGCAACCC | 60 | 130 | 0.99 |
| TBA_RV 2 | CCATTGTAGCACGTGTGTAGCC |
1 Degenerated bases, reported as indicated in IUPAC base coding, are underlined. 2 [21].