Figure 7.

Estimates of total cellular oxidized and reduced glutathione in the glycerol—(A) and glucose—(B) utilizing E. magnusii yeast in different growth stages. The harvested yeast cells were briefly washed with deionized water and frozen in liquid nitrogen for further HPLC analysis. 100 µL of re-frozen yeast lysate was added to 500 µL of 0.1 M cold iced PCA. The obtained cell homogenate was twice centrifuged for 20 min at 14,000 rpm in precooled (+4 °C) microcentrifuge. Then, 200 µL of clear supernatant was loaded into the HPLC vial for direct HPLC analysis. Values are mean ± SEM from 5–6 independent experiments. Error bars represent the standard deviation of triplicates to each experiment. a, b, c, d—p < 0.05.