Table 3.

Physiological parameters for the P. pastoris strains. Physiological parameters of three P. pastoris strains expressing CH3H as well as one empty strain. Standard deviations were calculated from triple measurements and calculated by error propagation. Differences in the maximum specific methanol uptake rates were determined significant between Mut+ and MutS strains at a confidence level of 95 %. In the SMD1168H cultivation strong methanol accumulation occurred after 48 h of induction. Therefore, the feed‐rate was reduced. However, the biomass concentration continuously decreased as a result of feeding close to the maintenance level. This resulted in an extremely low biomass yield and high standard deviation thereof. A star in the table marks respective parameters. Abbrevations: μ_max,gly: maximum growth rate on glycerol; q_s,max,gly: maximum specific glycerol uptake rate; Y_X/S: biomass yield on the respective substrate Y_CO2/S: CO₂ yield on the respective substrate; C‐Balance: carbon balance; t_adapt: adaption time to methanol; q_s,adapt: specific methanol uptake rate during adaption time; q_s,max,meoh: maximum methanol uptake rate

	Phase	Parameter	GS115 CH3H	SMD1168H CH3H	KM71H CH3H	KM71H empty
Gly	Batch	μmax,gly [h‐1]	0.21 ± 0.02	0.25 ± 0.06	0.29 ± 0.02	0.26 ± 0.02
		qs,max,gly [gs/gx/h]	0.38 ± 0.03	0.45 ± 0.11	0.54 ± 0.03	0.47 ± 0.03
	Fed‐batch	YX/S [C‐mol/C‐mol]	0.74 ± 0.05	0.75 ± 0.16	0.63 ± 0.06	0.67 ± 0.02
		YCO2/S [C‐mol/C‐mol]	0.33 ± 0.03	0.33 ± 0.02	0.32 ± 0.06	0.32 ± 0.05
		C‐Balance[‐]	1.07 ± 0.07	1.08 ± 0.14	0.95 ± 0.12	0.99 ± 0.03
MeOH	0.5 % Pulse	tadapt [h]	0.22	0.26	2.25	2.38
		qs,adapt [gs/gx/h]	0.024 ± 0.002	0.028 ± 0.002	0.029 ± 0.002	0.027 ± 0.002
	1 % Pulse	qs,max,meoh [gs/gx/h]	0.066 ± 0.005	0.072 ± 0.004	0.039 ± 0.002	0.054 ± 0.004
	Fed‐batch	YX/S [C‐mol/C‐mol]	0.16 ± 0.06	0.02 ± 0.29*	0.39 ± 0.08	0.38 ± 0.03
		YCO2/S [C‐mol/C‐mol]	0.87 ± 0.07	0.99 ± 0.25*	0.80 ± 0.04	0.77 ± 0.09
		C‐Balance[‐]	1.03 ± 0.11	1.02 ± 0.21*	1.19 ± 0.10	1.15 ± 0.11