In Table 2, the sequence of primer HpR3 is incorrect. The correct sequence of primer HpR3 is 5´ AAAACCAACAAAGGCCCGAA 3´. Please see the correct Table 2 here.
Table 2. Characteristics of the primers used for PCRs.
| Primer | Sequence (5´- 3´) | Fragment size (bp) | Temperature annealing (°C) | Reference |
|---|---|---|---|---|
| 18SFr | CGAGCAATAACAGGTCTGTG | 200 | 50°C | Mauri et al 2011 |
| 18SRw | GGCAGGGACTTAATCAA | |||
| HPNF3 | CATTAGCATGGAATAATAAAACACGAC | 600 | 55°C | Catanese et al 2018 |
| HPNR3 | GCGACGGCTATTTAGATGGCTGA | |||
| HpF | GGTACGGAGAATCCGGGGTT | 1409 | 55 °C | This study |
| HpR | ACTTGTCCTTCCTCTAATAATAAGG | |||
| HpF3 | GCGGGCTTAGTTCAGGGG | 165 | 60 °C | This study |
| HpR3 | AAAACCAACAAAGGCCCGAA |
Reference
- 1.López-Sanmartín M, Catanese G, Grau A, Valencia JM, García-March JR, Navas JI (2019) Real-Time PCR based test for the early diagnosis of Haplosporidium pinnae affecting fan mussel Pinna nobilis. PLoS ONE 14(2): e0212028 10.1371/journal.pone.0212028 [DOI] [PMC free article] [PubMed] [Google Scholar]