FIG 6.

Effects of ATR and CHK1 inhibitors on CCC DNA formation during HBV infection in PXB cells. The PXB cells were infected with HBV and treated with the CHK1 inhibitor CHIR-124 at the indicated concentrations. HBV PF DNA was extracted from the cells 3 days after infection and measured by Southern blotting using a ³²P-labeled HBV DNA probe. (A and B) Representative Southern blot autoradiograms of PF DNA extracted from two different batches of PXB cells infected with HBV. The brackets indicate the putative RC DNA processing products accumulating under conditions of CHK1 inhibition (see Fig. 7 below). The CCC DNA levels are indicated at the bottom, with that from the mock-treated cells set to 1.0. The vertical thin lines in the images denotes where the different parts of the same gel, and with the same exposure, were spliced together in order to remove other parts of the gel that are not presented here.