In the original article, there was a mistake in Table 1 as published. Due to a copy-paste error, the accession numbers, PCR primers, and amplicon sizes given for two of the RT-qPCR assays, mapk1 and casp3a, were wrong. Also, the accession number for dnmt1 was incorrect. The corrected Table 1 appears below. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Table 1.
PCR primers, accession numbers, amplicon sizes, and PCR efficiencies.
| Gene symbol | Gene name | Potential marker for | Accession no. | Forward primer | Reverse primer | Amplicon size (bp) | PCR efficiency |
|---|---|---|---|---|---|---|---|
| dnmt1 | DNA (cytosine-5-)- methyltransferase 1 |
DNA methylation | NM_131189 | GGGCTACCAGTGCACCTTTG | GATGATAGCTCTGCGTCGAGTC | 76 | 1.91 |
| dnmt3aa | DNA (cytosine-5-)- methyltransferase 3A |
DNA methylation | NM_001018134 | GGCGCCTGTTCTTTGAGTTT | TCACTGACCCCCATTGCAA | 112 | 1.91 |
| dnmt3b | DNA (cytosine-5-)- methyltransferase 3B |
DNA methylation | NM_001020476 | AGGTTTGGAACCTCCCGAAA | TGCGCACAGGTAACAAATGG | 115 | 1.94 |
| cbs | Cystathionine-beta-synthase | Transsulfuration | NM_001111232 | CTTTGCCCTGGTGGTTCATG | ACCACTCCAAACACCATTTGC | 81 | 2.00 |
| mgmt | O-6-methylguanine-DNA methyltransferase | DNA repair | NM_001256243 | TCCACCCTGTTGTCCTGTCA | GATGTAAGGCAGGCAGAGGAA | 117 | 2.03 |
| pgrmc1 | Progesterone receptor membrane component 1 | Glucose/Energy metabolism | NM_001007392 | TTTTCACGTCGCCACTGAAC | CTCCTCAACCGGGCCATAGT | 104 | 1.90 |
| cyp1a1 | Cytochrome P450 family 1 subfamily A member 1 | Detoxification | AF210727 | GGTGTTGGTTTTCGGTTTGG | GGCATCCCGGTGAACTTTAA | 114 | 1.99 |
| vtg1 | Vitellogenin 1 | Endocrine disruption | NM_001044897 | GTCATCAATGAGGATCCAAAGGCCA | GCCTCAGCGATCAGTGCACCAT | 209 | 1.91 |
| esr1* | Estrogen receptor 1 | Endocrine disruption | NM_152959 | AAACACAGCCGGCCCTACAC | GCCAAGAGCTCTCCAACAAC | 157 | 2.12 |
| esr2a* | Estrogen receptor 2a | Endocrine disruption | NM_180966 | TGATCAGCTGGGCCAAGAAG | GATTAACGGAGCGCCACATC | 123 | 2.00** |
| ar | Androgen receptor | Endocrine disruption | NM_001083123 | GGATGAGGTCGGAGCAGTTC | GGCTCAATGGCCTCCAGAAT | 117 | 2.03 |
| cyp19a2 | Cytochrome P450 family 19 subfamily A member 2 | Endocrine disruption | AF406756 | GAGCGGGCAGGACATAGTGT | GCTTGGGCTCAATCACTCTCA | 89 | 2.10 |
| fos | Fos proto-oncogene | Cell proliferation, differentiation and transcription regulation | NM_205569 | GGGTATTACCCGCTCAACCA | CAAGTCCGGGCATGAAGAGA | 102 | 2.02 |
| mapk1 | Mitogen-activated protein kinase 1 | Cell proliferation, differentiation and survival | NM_182888 | TACATCGGAGGAGGCGCTTA | GCTCAAACGGGCTGATCTTC | 94 | 1.99 |
| casp3a | Caspase 3A | Apoptosis | NM_131877 | CCCAGATGGTCGTGAAAGGAT | TGAACCATGAGCCGGTCATT | 107 | 2.07 |
| eef1a1 | Eukaryotic translation elongation factor 1 alpha 1 | Refgen | AY422992 | AGACAACCCCAAGGCTCTCA | CTCATGTCACGCACAGCAAA | 126 | 2.06 |
| uba52 | Ubiquitin A-52 residue ribosomal protein fusion product 1 | Refgen | NM_001037113 | CGAGCCTTCTCTCCGTCAGT | TTGTTGGTGTGTCCGCACTT | 126 | 2.08 |
| actb | Beta-actin | Refgen | AF057040 | CGAGCAGGAGATGGGAACC | CAACGGAAACGCTCATTGC | 102 | 2.08 |
*PCR primers obtained from Sawyer et al. [93]. **PCR efficiency set to 2.00.