Table 1. Predicted aggregation behavior of cancer and neurodegenerative proteins.
| Method Protein |
Aggrescan | Tango | Waltz | Zyggregator |
|---|---|---|---|---|
| Mouse PrP | nHS: 3 NnHS: 1.435 |
nHS: 1 Agg: 61.2177 |
nHS: 4 | Zagg: 0.998 Ztox: 0.107975 |
| Human p53 | nHS: 8 NnHS: 2.036 |
NHS: 2 Agg: 761.196 |
nHS: 1 | Zagg: 0.909 Ztox: -1.51057 |
| Human p63 | nHS: 10 NnHS: 1.767 |
* | nHS: 3 | Zagg: 0.709 Ztox: -1.51621 |
| Human p73 | nHS: 9 NnHS: 1.804 |
* | nHS: 5 | Zagg: 0.842 Ztox: -0.904242 |
| Aβ 1–42 | nHS: 2 NnHS: 4.762 |
NHS: 2 Agg: 1598.46 |
nHS: 2 | Zagg: 0.975 Ztox: 0.504339 |
| α-synuclein | nHS: 6 NnHS: 4.286 |
NHS: 4 Agg: 896.077 |
nHS: 1 | Zagg: 0.980 Ztox: -0.829966 |
| SOD1 | nHS: 6 NnHS: 3.896 |
NHS: 1 Agg: 145.586 |
nHS: 1 | Zagg: 0.862 Ztox: -0.136983 |
AGGRESCAN predicts aggregation-prone segments. WALTZ predicts amyloid-forming sequences. ZYGGREGATOR predicts different phases of the aggregation process. nHS: number of Hot Spots. NnHS, Normalized nHS for 100 residues; Agg, the propensity for β-sheet aggregation; Zagg, the tendency to convert from the unfolded state to the amyloid state; Ztox, the tendency to convert from the unfolded state to oligomeric assemblies. Zagg and Ztox values close to 1 represent a high aggregation propensity. WALTZ was performed with Best Overall Performance threshold. Conditions were as follows: pH 7.0, 298.15 kelvin and 0.02 M (ionic strength). *TANGO limits analysis to 500 amino acids.