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Schematic representation of the modified Western blotting method. (A) Modified 3-step Western blotting method including the blocking of endogenous biotin method. (B) Diagram showing how antigens are detected using the conventional-HRP, PolyHRP, Streptavidin-conjugated PolyHRP (C) Schematic representation of Streptavidin-conjugated PolyHRP20 and Streptavidin-conjugated PolyHRP80. Ab, antibody; TBST, tris buffered saline containing tween 20; RT, room temperature.
