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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Electrophoresis. 2019 May 9;40(12-13):1731–1739. doi: 10.1002/elps.201900059

Figure 4.

Figure 4.

Western Blots of rat heart lysates probed for proteasome PSMA6 and PSMA3 subunits using conventional HRP, PolyHRP and Streptavidin-conjugated PolyHRP20s. (A) Blot showing detection of PSMA6 in 10μg of heart lysate using conventional HRP and PolyHRP (goat anti rabbit HRP). (B) Quantification of PSMA6 on blots using HRP and PolyHRP. (C) Blot showing detection of PSMA6 in 10μg of heart lysate using PolyHRP and Strep-PolyHRP20. (D) Quantification of PSMA6 on blots using PolyHRP and Strep-PolyHRP2. (E) Blots of PSMA3 using conventional HRP and PolyHRP in 2μg of heart lysate. (F) Quantification of PSMA3 using conventional HRP and PolyHRP in 2μg and 10μg of heart lysate. (G) Quantification of PSMA3 using conventional HRP and PolyHRP in 10μg of heart lysate. Ponceau S staining of the blots was used for normalization of the results. Values are means ± SE; n = 3 per group. *P<0.05, **P<0.01.