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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Electrophoresis. 2019 May 9;40(12-13):1731–1739. doi: 10.1002/elps.201900059

Figure 5.

Figure 5.

Western Blots incorporating a blocking step for Strep-HRP and Strep-PolyHRP20 for 500ng, 2μg, and 10μg of heart lysate. (A) Modified Streptavidin-biotin blocking for Strep-HRP. (B) Effect of the modified blocking step on blocking endogenous biotin as well as 10 mM of exogenous biotin in heart lysates. (C) Blocking using a pre-hybridization method for Strep-PolyHRP20 for PSMA3; (D) Modified Streptavidin-biotin blocking step for PSMA6 with Strep-PolyHRP20. n = 3 per group. *P<0.05, **P<0.01.