Table 3.
Accuracy and precision of SH-1242 determinations in rat and mouse quality control (QC) samples.
| Batch | Rat Plasma | Mouse Plasma | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Theoretical Concentration (ng/mL) | Theoretical Concentration (ng/mL) | |||||||||
| LLOQ | LQC | MQC | HQC | HQC a | LLOQ | LQC | MQC | HQC | HQC a | |
| 1 | 2 | 40 | 800 | 800 | 2 | 4 | 40 | 800 | 800 | |
| Intra-day (n = 6) | ||||||||||
| Mean | 1 | 1.98 | 41.9 | 759 | 764 | 1.95 | 4.06 | 39.7 | 752 | 757 |
| Precision (CV%) b | 12.7 | 4.64 | 4.41 | 3.85 | 1.39 | 2.56 | 2.55 | 2.96 | 3.09 | 4.47 |
| Accuracy (RE%) c | 0.38 | −0.83 | 4.83 | −5.15 | −4.48 | −2.42 | 1.42 | −0.83 | −6.02 | −5.35 |
| Inter-day (n = 30) | ||||||||||
| Mean | 1.04 | 2.04 | 41.9 | 777 | 743 | 1.99 | 4.04 | 40.7 | 780 | 782 |
| Precision (CV%) b | 12.8 | 5.04 | 5.03 | 4.24 | 2.84 | 5.87 | 4.43 | 3.57 | 5.27 | 6.72 |
| Accuracy (RE%) c | 3.95 | 2.22 | 4.63 | −2.83 | −7.15 | −0.73 | 0.91 | 1.63 | −2.53 | −2.30 |
a Analyzed after a ten-fold dilution with blank plasma. b CV(%) = (standard deviation/mean) × 100. c RE(%) = [(calculated concentration – theoretical concentration)/theoretical concentration] × 100. RE: relative error, LQC and MQC: low and mid QC.