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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Proteomics. 2019 Dec 23;20(1):e1900266. doi: 10.1002/pmic.201900266

Figure 4.

Figure 4.

Validation of alcohol-induced protein expression change in the NAc of Ng−/− mice using western blotting. A. Alcohol did not induce any significant changes in Ca2+-CaMKII signaling in the NAc of Ng–/– mice. There is no difference in CaMKII-mediated phosphorylation in pNR2B(S1303) and pGluR1(S831). B. Alcohol did not induce any changes in PKCγ signaling and pNR1(S896) phosphorylation. C. After alcohol treatment, AKT phosphorylation in the Ng−/− mice was significantly increased, while mGluR5 expression was significantly decreased in Ng–/– mice (n = 4). *p < 0.05 unpaired t-test for protein expression between genotypes and protein expression was normalized by GAPDH expression. All data are presented as mean ± SEM.