Table 1. Quality metrics of Unigenes.
Sample | Total number | Total length | Mean length | N50 | N70 | N90 | GC (%) |
---|---|---|---|---|---|---|---|
HL_1 | 47,956 | 48,017,129 | 1,001 | 1,597 | 1,038 | 422 | 43.49 |
HL_2 | 42,932 | 40,607,825 | 945 | 1,520 | 961 | 388 | 43.43 |
HL_3 | 42,861 | 40,604,125 | 947 | 1,514 | 959 | 391 | 43.6 |
LL_1 | 72,845 | 71,641,113 | 983 | 1,631 | 1,007 | 391 | 41.59 |
LL_2 | 59,271 | 56,435,088 | 952 | 1,589 | 987 | 376 | 42.65 |
LL_3 | 61,471 | 58,975,317 | 959 | 1,594 | 998 | 380 | 42.47 |
ML_1 | 51,245 | 51,521,358 | 1,005 | 1,626 | 1,054 | 417 | 43.03 |
ML_2 | 48,597 | 49,086,125 | 1,010 | 1,619 | 1,059 | 421 | 43.33 |
ML_3 | 60,239 | 53,701,642 | 891 | 1,510 | 896 | 344 | 42.54 |
Note:
N50, the N50 length is used to determine the assembly continuity, the higher the better; N70, similar to N50; N90, similar to N50; GC (%), the percentage of G and C bases in all Unigenes.