Figure 1.

Treatment with COTI-2 results in caspase activation and apoptosis in dose- and in time-dependent manners in Jurkat cells. (a), Jurkat cells were treated with 0, 50, 100, 150, and 200 nM COTI-2 for 48 h. (b) The cells were treated with 200 nM BITC for 0–48 h. In (a) and (b), cells were stained with Annexin V/PI, and the percentage of apoptotic cells was determined using flow cytometry. (c,d), After treatment with COTI-2, total cellular extracts, nuclear extracts, and cytosolic fractions were prepared and subjected to western blot analysis using antibodies against PARP, cleaved-caspase (C-Caspase)-9, cleaved-caspase-3. Each lane was loaded with 30 mg of protein. Two additional studies yielded equivalent results.