Figure 4. DAF-2B influences dauer recovery in temperature-sensitive insulin-signaling mutants.

(A) Exit from the dauer larva into a reproductive adult requires an increase in the activity of insulin agonists. Overexpression of DAF-2B from the native daf-2 promoter (B), in the nervous system (C), in the hypodermis (D), in muscle (E) or in the intestine (F) inhibits dauer recovery in daf-2 mutants under permissive conditions. Data are pooled from three transgenic lines (except two for hypodermal and muscle promoters) with at least three technical replicates per transgene. Two additional trials with native, neuronal, muscle and intestinal DAF-2B OE showed similar effects. Raw data can be found in Figure 4—source data 1. (G) Genetic deletion of daf-2b promotes dauer exit in pdk-1 mutants. Data are pooled from six technical replicates from one control and one daf-2b(Δ) strain. Two biological repeats showed similar effects. Raw data can be found in Figure 4—source data 2. (H) Genetic deletion of daf-2c has no effect on dauer exit in pdk-1 mutants. Data are pooled from three technical replicates from each of 2 control and daf-2c(Δ) strains per trial. Two biological repeats showed similar effects. Log Rank test ns – not significant, **p<0.01, ***p<0.001. Raw data can be found in Figure 4—source data 2.