The retraction notice [1] for this article [2] included the following statements: ‘The western blot experiments shown in Fig 4 did not include general loading controls, and the authors did not report total Rb controls for the phospho-Rb (pRb) experiments’, ‘Furthermore, the lack of protein loading controls for the experiments in Figure 4 call into question the validity of the results.’
After publication of the notice, it was clarified that in Figure 4 of [2] “pRb” referred to total retinoblastoma protein rather than a phosphorylated form of this protein. As such, this point in the retraction notice is incorrect: “the authors did not report total Rb controls for the phospho-Rb (pRb) experiments.”
In addition, the authors noted that the total ERK and total Akt panels in Figure 4 provided information about protein loading across lanes. Additional loading controls were not requested during the re-evaluation of the article, the authors noted that such controls could in principle have been provided had they been requested.
References
- 1.The PLOS ONE Editors (2019) Retraction: EGFR Inhibition in Glioma Cells Modulates Rho Signaling to Inhibit Cell Motility and Invasion and Cooperates with Temozolomide to Reduce Cell Growth. PLoS ONE 14(9): e0222210 10.1371/journal.pone.0222210 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Ramis G, Thomàs-Moyà E, Fernández de Mattos S, Rodríguez J, Villalonga P (2012) EGFR Inhibition in Glioma Cells Modulates Rho Signaling to Inhibit Cell Motility and Invasion and Cooperates with Temozolomide to Reduce Cell Growth. PLoS ONE 7(6): e38770 10.1371/journal.pone.0038770 [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
