Clinical use of 4D flow MRI for quantification of aortic regurgitation

Abstract

Objective

The main objective of the present study was to compare the use of four-dimensional (4D) flow MRI with the habitual sequence (two-dimensional phase-contrast (2DPC) MRI) for the assessment of aortic regurgitation (AR) in the clinical routine.

Methods

This was a retrospective, observational cohort study of patients with varying grades of AR. For the purposes of the present study, we selected all the cases with a regurgitant fraction (RF)>5% as determined by 2DPC MRI (n=34). In all cases, both sequences (2DPC and 4D flow MRI) were acquired in a single session to ensure comparability. We compared the results of the two techniques by evaluating forward flow, regurgitant flow and regurgitation fraction. Then, the patients were divided into subgroups to determine if these factors had any influence on the measurements: aortic diameter (≤ vs >38 mm), valve anatomy (tricuspid vs bicuspid/quadricuspid), stenosis (gradient ≥15 vs <15) and region of interest location (aortic valve vs sinotubular junction).

Results

No statistically significant differences were observed between the two techniques with Pearson’s correlation coefficients (r) of forward flow (r=0.826/p value<0001), regurgitant flow (r=0.866/p value<0001) and RF (r=0.761/p value<0001).

Conclusions

The findings of this study confirm the value of 4D flow MRI for grading AR in clinical practice with an excellent correlation with the standard technique (2DPC MRI).

Key messages.

What is already known about this subject?

• Four-dimensional (4D) flow MRI allows to measure non-laminar flow in any direction in space, with flow encoding in all three spatial directions along the cardiac cycle, overcoming one of the several limitations of two-dimensional, phase-contrast (2DPC) MRI. Several recent studies have verified the validity of 4D flow MRI compared with both echocardiography and 2DPC MRI. Some authors found that 4D flow MRI and echocardiography presented good interobserver correlation, with good agreement in assessing valvular regurgitation. Flow and velocity measurements obtained by 4D flow MRI in mediastinal vessels have been successfully compared in healthy patients.

What does this study add?

• To our knowledge, despite the prevalence of aortic regurgitation (AR), there is not a study comparing 4D flow MRI with 2DPC MRI for grading AR in routine clinical practice. We found that there is a good correlation between the two sequences to quantify aortic positive and negative flow and aortic regurgitant fraction.

How might this impact on clinical practice?

• From our point of view, when prospective studies also verified the prognostic information obtained from 4D flow MRI, this sequence will substitute 2DPC MRI in the clinical protocol of cardiac magnetic resonance (CMR). We have seen its good correlation with the habitual sequence for grading AR, one of the most common valvular pathologies studied with MRI. Also, with the numerous advantages of the sequence, the indications of MRI will increase in valvular pathologies.

Introduction

Aortic regurgitation (AR) is the diastolic flow of blood from the aorta to the left ventricle (LV), which leads to volume and pressure overload on the LV. According to recent clinical guidelines,^{1 2} echocardiography is the imaging technique of choice for the diagnosis, quantification and follow-up of AR.

Echocardiography provides data on the most relevant prognostic parameters—LV dilatation and/or dysfunction—needed to monitor patients with AR.^1–3

MRI is considered a complementary technique in patients with AR with inadequate echocardiographic image quality or discrepant results. MRI provides more precise estimates of LV volumes, function and mass, and aortic dilatation than echocardiography.^{1 4 5}

Studies have shown that the regurgitant fraction (RF) can be directly measured using two-dimensional, phase-contrast (2DPC MRI), whose diagnostic capacity has been known since 1960.⁶ 2DPC MRI can also measure flow, velocity and direction accurately and reproducibly,⁷ even considering the limitations⁸ of 2DPC MRI (eg, the assumption of laminar flow and a dependency on the flow intercept angle). In fact, some studies have shown that 2DPC MRI could be superior to transthoracic echography in quantifying AR for prognostic purposes.^9–11

For this reason, 2DPC MRI is included in routine MRI protocols.

Three-dimensional (3D) flow MRI is a technique known around for 20 years but recent developments in image acquisition protocols, as well as dedicated postprocessing tools, have contributed to the recent implementation of this approach in clinical workflow.

3D flow MRI with these new developments, known nowadays as four-dimensional (4D) flow MRI (3D within the time of the cardiac cycle), has emerged as an alternative clinical approach to measuring blood flow. The clinical validity of 4D flow MRI to evaluate several relevant parameters has been demonstrated, although additional studies are needed to confirm its capacity to assess other parameters such as wall shear stress.¹² This technique allows to measure non-laminar flow in any direction in space, with flow encoding in all three spatial directions along the cardiac cycle, overcoming one of the several limitations of 2DPC MRI (single velocity encoding direction perpendicular to 2D plane), thus offering new possibilities for MRI in the assessment of AR.

Several recent studies^13–15 have verified the validity of 4D flow MRI compared with both echocardiography and 2DPC MRI. Hsiao et al ¹⁶ found that 4D flow MRI and echocardiography presented good interobserver correlation, with good agreement in assessing valvular regurgitation. Flow and velocity measurements obtained by 4D flow MRI in mediastinal vessels have been successfully compared in healthy patients¹⁶ and in patients with congenital heart disease.¹⁷

To our knowledge, despite the prevalence of AR, there is not a study comparing 4D flow MRI with 2DPC MRI for grading AR in routine clinical practice.

In this context, the main objective of the present study was to compare the use of 4D flow MRI with the habitual sequence (2DPC MRI) (already validated compared with ultrasound) for the assessment of AR in the clinical routine. More specifically, our aim was to determine if these two imaging modalities are equivalent with regard to AR quantification.

We hypothesised that the regurgitant flow data obtained by 4D flow MRI would not differ significantly from that obtained by 2DPC MRI. Furthermore, we expected that 4D flow MRI would permit an accurate grading of AR and would replace 2DPC in the clinical cardiac MRI protocol.

Secondary objectives were to determine whether anatomical variations (aortic diameter, valve anatomy) or other variables (presence of stenosis, location of region of interest (ROI)) affect the comparability of the quantification of AR severity with the two sequences.

Material and methods

Type of study

This was a retrospective, observational cohort study of patients with varying grades of AR. All participants were treated at the Quirónsalud Madrid University Hospital in Madrid, Spain.

This study received no funding.

Study population

We retrospectively analysed the medical records of 220 patients who underwent both 4D flow MRI and 2DPC MRI between May 2017 and March 2018 at our institution.

For the purposes of the present study, we selected all the cases with an RF >5% as determined by 2DPC MRI (n=34).

Exclusion criteria were as follows: (1) aortic valve or root replacement and (2) poor quality imaging studies in which the 2DPC and/or 4D flow sequence was not evaluable, or in which the studies were incomplete (patient failure to complete the test or refusal to accept intravenous contrast).

All patients in the study met the relevant clinical criteria for MRI. Prior to study inclusion, all patients completed a questionnaire to provide data on medical and surgical history, medications, allergies and implants (eg, pacemaker or metal implants). The study procedure was explained in detail to the patients, including the risks and the test duration, prior to obtaining signed informed consent.

MRI protocol

The MRI studies were performed using the GE Signa 1.5T MRI (GE Medical Systems, Milwaukee, Wisconsin, USA) with a 32-channel surface coil. As part of standard procedures, most of the imaging studies included standard cine and delayed-enhancement MRI sequences to assess for the presence of myocardial fibrosis.¹⁸ In all cases, both sequences (2DPC and 4D flow MRI) were acquired in a single session to ensure comparability.

2DPC MRI

Acquisition

For image acquisition of the 2DPC MRI sequence, we used a single double oblique planned acquisition plane orthogonal to the wall of the aorta. There is some disagreement with regard to the optimal imaging plane to assess AR. Some authors propose placing the plane at the sinotubular junction or the proximal ascending aorta,¹⁹ while other authors advocate placement at the middle ascending aorta¹⁵ or below the aortic valve.²⁰ At our institution, the slices are placed at the valve or the sinotubular junction, depending on the anatomical characteristics of the patient.

A fast, two-dimensional sequence was used with the patient in end-expiratory breath-hold with retrospective cardiac gating (30 reconstructed phases per cardiac cycle) and k-space segmentation of four views/segment. Other parameters were as follows: matrix, 192×128; field of view, 420×294 mm and slice thickness, 5 mm (without spacing). As we used a fixed four views/segment, the true temporal resolution in data acquisition is 45.6 ms. Repeat and echo times were 5.7 and 3.5 ms, respectively, with a 15° flip angle. The acquisition time was approximately 14 s. In all cases, velocity encoding (VENC) was initially set at 150–200 cm/s and progressively increased by 50 cm/s until the image contained no artefacts. The lowest VENC at which no velocity aliasing is observed was selected.⁸

Processing

The data were processed with the CardiacVX software for GE Advantage Workstation V.4.4 (GE Healthcare, USA), which offers a semiautomatic analysis in the plane acquired during the test. An experienced radiologist (>11 years of experience), working in collaboration with a more experienced radiologist (>35 years of experience), place an ROI at the aorta in the acquired plane, verifying that the propagation adapts to the anatomical limits, to obtain a flow-time curve.

4D flow MRI

Acquisition

Prior to sequencing, a peripheral intravenous line is started to administer the gadolinium-based (Gadobutrol) contrast agent (Gadovist 1 mmol/mL; Bayer, Mijdrecht, The Netherlands). Image acquisition begins simultaneously with the injection of 0.15 mmol/kg of contrast agent followed immediately by saline flush. The dose of the contrast agent is based on current recommendations of the Society for Cardiovascular Magnetic Resonance.²¹

The use of contrast is not necessary but allows faster scanning. We use gadolinium-based agent in our clinical MRI protocol to obtain late gadolinium enhancement imaging. Acquiring the 4D flow CMR data after the study that requires contrast administration takes advantage of the enhanced signal-to-noise ratio and thus velocity-to-noise ratio, as well as contrast between blood and surrounding tissue.¹²

A 3D volume is acquired from the cardiac apex to the aortic arch with the patient in free-breathing and retrospective ECG-cardiac gating. The sequence adapts the views/segment automatically based on patient heart rate (HR) (smaller with HR and vice versa) to ensure 16 cardiac phases are acquired per cardiac cycle. The reconstructed images are linearly interpolated into 30 cardiac phases for better visualisation of the temporal variation. The acquisition time ranges from 7 to 10 min.

In all cases, VENC was the same as that used in the 2DPC MRI. The technical characteristics of this sequence are summarised in table 1.

Table 1.

Acquisition parameters in 4D flow MRI

Acquired resolution (mm)	2.78×2.78×2.40 (RL×AP×IS)
Reconstructed resolution (mm)	1.95×1.95×1.20 (ZIP×2)
Average scan time (min:s)	7:30
Average VENC (cm/s)	160
Views per segment	Adaptative to patient HR
FA	15°
TR	4.25
TE	2.25
Phases to reconstruct	30

FA, flip angle; HR, heart rate; TE, echo time; TR, repetition time; VENC, velocity encoding.

Processing

The raw data are sent directly to a cloud software application (Arterys, San Francisco, California, USA) for image reconstruction and data correction. This application also provides real-time, 3D postprocessing tools to quantify function and flow. This image analysis is anonymised, and both automatic and semiautomatic analyses are available. The flow is visualised with a superimposed colour code based on the velocity of the anatomical data. Tools are available to define flow direction and trajectory (streamlines and vectors).

For the quantitative analysis, the same two radiologists—without access to the 2DPC MRI analysis or the patient’s personal data—place the ROI (by consensus agreement) in a double plane orthogonal to the ascending aorta in the same plane used for 2DPC MRI acquisition. The ROI propagates throughout the cardiac cycle (automatic or manual corrections can be made in each frame if necessary), thus verifying that the ROI adapts to the vessel in all 30 phases of the cardiac cycle.

Comparison: 2DPC MRI versus 4D flow MRI

We compared the overall results of the two techniques by evaluating three variables: forward flow, regurgitant flow and RF. Also, we compare the grading (based on RF) between the two techniques.

Then, the patients were divided into subgroups to determine if these factors had any influence on the measurements: aortic diameter (≤ vs >38 mm), valve anatomy (tricuspid vs bicuspid/quadricuspid), stenosis (gradient ≥15 mm Hg vs <15 mm Hg) and ROI location (aortic valve vs sinotubular junction).

Statistical analysis

The qualitative variables were expressed as absolute (n) and relative (%) frequencies. The Shapiro-Wilk test was applied to evaluate the parametric behaviour of the quantitative variables. Mean values±SD are given for normal distributions; for non-normal distributions, the data are reported as medians with IQR.

Pearson’s (r) and Spearman’s (rho) correlation coefficients were calculated for parametric and non-parametric data, respectively, to determine correlations between the two techniques. For qualitative variables, the Student’s t-test or Mann-Whitney U-test (depending on the normality distribution) was applied to analyse differences between the values obtained in the two techniques. The χ² test or Fisher’s exact test was used for qualitative variables. The differences were considered statistically significant for p<0.05. Data analysis was performed with the IBM-SPSS statistical software program, V.21.0.

Results

Baseline characteristics

Thirty-four patients (27 male; 79.4%) were included in the study. The median age was 58 (17.8) years. The descriptive parameters are shown in table 2. The mean (SD) maximum diameter of the ascending aorta was 40.8 (7.4) mm. In most cases, the aortic valve anatomy was tricuspid (21 cases; 61.8%) or bicuspid (12; 35.3%); one patient (2.9%) had a quadricuspid valve. The median LV ejection fraction was 58% (±12) and the mean end-diastolic diameter of the LV was 54 mm (±9).

Table 2.

Demographic data

	Total (n=34)
Gender
Male, n (%)	27 (79.4)
Female, n (%)	7 (20.6)
Age, median (IQR)	58.0 (17.8)
Anatomy
Bicuspid, n (%)	12 (35.3)
Tricuspid, n (%)	21 (61.8)
Quadricuspid, n (%)	1 (2.9)
Diameter (mm), mean±SD	40.8±7.4
Diameter grouped
<38 mm, n (%)	13 (38.2)
≥38 mm, n (%)	21 (61.8)
Other pathologies
No pathology, n (%)	29 (85.4)
Ascending aortic dissection, n (%)	1 (2.9)
Aortic coarctation (treated), n (%)	1 (2.9)
Hypertrophic cardiomyopathy, n (%)	2 (5.9)
LV apical infarction with thrombus attached, n (%)	1 (2.9)
LVEF (%), median (IQR)	58.0 (12.0)
LVEF grouped
Decreased, n (%)	5 (15.2)
Normal, n (%)	28 (84.8)
EDD (cm), mean±SD	5.4±0.9
EDD grouped
Dilated, n (%)	14 (42.4)
Pathological, n (%)	19 (57.6)

EDD, End diastolic diameter; LV, left ventricle; LVEF, Left ventricle ejection fraction.

Comparison between 2DPC MRI and 4D flow MRI

No statistically significant differences were observed between the two techniques with regard to quantification of forward flow, regurgitant flow or RF (figures 1 and 2).

Figure 1.

2DPC vs 4D flow. Lineal correlation between positive flow, negative flow and RF. Depicts the good correlation between the measurements without statistical differences (2D flow on y-axis) versus 4D flow (x-axis).

Figure 2.

2DPC vs 4Dflow: Comparison of quantitative parameters.

The severity grading obtained by 2DPC MRI and 4D flow MRI differ in 11 of the 34 patients in our cohort (table 3), with 2DPC MRI overestimating severity compared with 4D flow MRI in 10 of those 11 cases, probably because regurgitant flow and RF values were higher when measured by 2DPC MRI.

Table 3.

Comparison of AR grading (based on FR) between 2DPC MRI and 4D flow MRI

		4D flow				Upstalling	Dowstalling
		<=15%	16%–25%	26%–48%	>48%	2DPC to 4D flow	2DPC to 4D flow
2DPC	<=15%	13	0	0	0	0 (0.0%)	0 (0.0%)
	16%–25%	5	4	1	0	1 (10.0%)	5 (50.0%)
	26%–48%	2	2	5	0	0 (0.0%)	4 (44.4%)
	>48%	0	0	1	1	0 (0.0%)	1 (50.0%)

AR, aortic regurgitation; 4D, four dimensional; 2DPC, two-dimensional, phase-contrast.

There were no significant between-group differences in the quantitative values obtained by the two techniques (figure 3) in any of the subgroup comparisons. Similarly, we found no significant differences between the two subgroups defined according to ROI location (aortic valve vs sinotubular junction) in any of the variables (table 4).

Figure 3.

Aortic dilatation. Depicts a Bland-Altman analysis with the difference between both RF measurements (2DPC and 4Dflow) on the y-axis and the average of both measurements on the x-axis in each subgroup.

Table 4.

ROI location. Correlation coefficients between positive flow, negative flow and RF using 2DPC MRI and 4D flow MRI, depending on the ROI location

	Valve	Correlation (r)	ST	Correlation (r)
	(n=19)	(95% CI)	(n=15)	(95% CI)
Positive flow (mL/lat)
4D flow, mean±SD	94.8±39.7	0.846 (0.637 to 0.939)	83.0±16.9	0.636 (0.183 to 0.866)
2DPC, mean±SD	96.1±41.5		79.4±18.1
Negative flow (mL/lat)
4D flow, median (IQR)	−7.6 (15.5)	0.790 (0.512 to 0.918)	−9.3 (16.1)	0.889 (0.684 to 0.964)
2DPC, median (IQR)	−16.9 (24.5)		−12.8 (24.7)
Percentage of regurgitation
4D flow, median (IQR)	11.6 (20.4)	0.726 (0.394 to 0.891)	12.9 (17.0)	0.861 (0.613 to 0.954)
2DPC, median (IQR)	18.2 (21.7)		17.0 (24.6)

4D, four-dimensional; 2DPC, two-dimensional, phase-contrast; RF, regurgitant fraction; ROI, region of interest.

Discussion

The main aim of this study was to compare 4D flow MRI with 2DPC MRI for the clinical assessment of AR.

For years, 4D flow MRI good internal consistency and good interindividual reproducibility have been well known.²² Also, 4D flow MRI has been demonstrated to improve the right ventricular valve flow and diastolic function quantification,²³ and it has even been used as a reference tool to compare the accuracy of 3D versus 2D echography for quantification of AR.²⁴

Several authors have validated the use of 4D flow MRI in different clinical situations: to determine morphological and haemodynamic values such as velocity peak and flow displacement,^{25 26} to asses severity in hypertrophic myocardiopathy (measuring peak velocity),²⁷ to asses dysfunction in bicuspid valves²⁸ or to detect incipient haemodynamic changes in Marfan patients.²⁹

Even, new automatic techniques for valve tracking have been validated, although not in AR.³⁰

To our knowledge, despite the prevalence of AR, there is not a study comparing 4D flow MRI with 2DPC MRI for grading AR in routine clinical practice. Our aim was to determine if these two imaging modalities are equivalent with regard to AR quantification and if we can confidently change the habitual MRI protocol in this common clinical context.

Importantly, we found no statistically significant differences between the two techniques in the quantitative assessments (figures 1 and 2). For the comparison, 2DPC MRI was used as the reference technique because this sequence has been previously validated by comparison with echocardiography for AR quantification.^{7 9–11}

Flow volume

Bollache et al found that net volumes measured with the patient in sustained breath-holding are lower than those acquired in, even when the same sequence is used.¹³ Another study showed that 4D flow MRI underestimates individual flow volume quantification compared with 2DPC MRI.³¹ In our study, we found no significant differences between 4D flow MRI and 2DPC MRI with regard to forward flow values.

RF and severity grading

Because RF depends on forward and regurgitant flow, small differences between 2DPC MRI and 4D flow MRI in these volume measurements explains why RF values measured by 2DPC MRI tend to be higher (figure 2).

Severity criteria for AR assessed by echocardiography, which include qualitative, semiquantitative and quantitative criteria, are well-established in clinical guidelines.¹ By contrast, the reference values for MRI are less well-established and different authors have described different values. For example, some authors have used the same threshold values for MRI as for echocardiography,³² while other authors have established different cut-off points. For example, Myerson et al ¹⁰ concluded that an RF>33% predicted the need for valve replacement.

Gelfand et al ³³ concluded that the cut-off points for RF that best correlate with echocardiography are as follows: mild AR (≤15%), moderate (16%–25%), moderate-severe (26%–48%) and severe (>48%).

If we apply these criteria—which provide narrower ranges that are useful in clinical practice—to our patient data, the severity grading obtained by 2DPC MRI and 4D flow MRI differ in 11 of the 34 patients in our cohort (table 3), with 4D flow MRI underestimating severity compared with 2DPC MRI in 10 of those 11 cases, probably because, as mentioned above, forward flow values tend to be lower when measured by 2DPC MRI (maybe because of the presence of non-laminar flow), whereas the 2DPC MRI regurgitant flow tend to be slightly higher.

Kappa agreement for classifying regurgitation can be calculated between both methods (kappa of 0.72), which means moderate agreement. Also, most of the cases with disagreement would be clinically classified as mild-moderate regurgitation with treatment depending on clinical predictors. Our hypothesis is that with 4D flow MRI, we would use other flow variables (turbulence, jet diameter and so on) that improve the AR grading. For this purpose, posterior studies are necessary.

Location of the ROI

In our study, we used a single double oblique planned acquisition plane orthogonal to the wall of the aorta for the 2DPC MRI acquisition.

However, the optimal location to most accurately measure AR is controversial^{19 20} because RF values vary depending on the plane that is used. Likewise, the stroke volume is also controversial. Chaturvedi et al ³⁴ found that the most accurate measures of cardiac output are obtained at the proximal ascending aorta; however, Bertelsen et al ³⁵ found a better correlation for stroke volume at the valve level. For this reason, we compared flow values obtained by placing an ROI in the same location for both MRI sequences (at the valve level or at the sinotubular junction), thus eliminating any possible influence of this variable on the comparative results. Although we found no statistically significant differences in any of the subgroups, the RF values obtained with the two techniques were more closely correlated when the ROI was located at the sinotubular junction (table 4), likely due to the decreased non-laminar flow in this area. This approach does not make use of the possible advantages of 4D flow MRI. We are aware that posterior studies are necessary to determine the best aortic plane for regurgitation quantification with 4D flow MRI for prognostic purposes.

Dilatation

In patients with dilated aortas and aortas with bicuspid valves, helical flow patterns differ from those observed in healthy patients, resulting in differences in flow measurements.^{36 37} In this regard, 4D flow MRI is believed to be more accurate than 2DPC MRI in measuring helical flow.³⁸ Studies have shown that measurements of cardiac output present substantially more variability in the presence of complex flow patterns secondary to aortic stenosis.³⁹ However, we found no significant differences in the values obtained by the two techniques in any of the subgroups analysed (dilated vs non-dilated aortas, tricuspid vs bicuspid/quadricuspid and stenotic vs non-stenotic). RF values were more closely correlated in smaller aortas (diameter 38 mm) (figure 3), probably due to non-laminar flow, but we were unable to assess correlations in the other subgroups due to the small number of patients in those groups.

The favourable findings of the present study suggest that the cardiac output values measured by 4D flow MRI and 2DPC MRI in routine clinical practice do not show statistically significant differences, and in the majority of the patients, the AR grading was concordant.

Also, we suggest that 4D flow MRI has several advantages over 2DPC MRI, particularly with regard to the assessment of complex flows in dilated aortas.

We believe that 4D flow MRI will, in the future, form part of standard MRI protocols replacing 2DPC MRI. However, additional clinical utility studies are needed to confirm these findings and to confirm the advantages of 4D flow MRI.

The advantages of 4D flow MRI we noticed in our initial practice can be summarised as follows: (1) 3D anatomical, functional and flow data; (2) acquisition during free breathing (2DPC MRI could be acquired with free breathing with navigator-based motion suppression); (3) capacity to retrospectively analyse any flow type (ie, laminar or non-laminar) included in the study, in any direction, compensating the longer duration of the sequence; (4) visualisation of complex or eccentric flows; (5) retrospective tracking of one or more jets to avoid underestimating the RF⁴⁰; (6) allows assessment of internal validity (eg, by comparing values in the pulmonary artery with those obtained in the aorta); and (7) it can also be used to identify holodiastolic flow reversal in the descending aorta, an independent predictor of severe AR.⁴¹

Study limitations and strengths

One of the limitations of this study is the relatively small number of patients. For this reason, larger studies with longer follow-up will be necessary to strengthen the clinical evidence for 4D flow MRI. Another limitation inherent to the two sequences is that 2DPC MRI must be acquired with the patient in breath-hold, whereas 4D flow acquisition is made in free breathing. An important strength is that this study is the first study to compare 4D flow MRI for AR in routine clinical practice with the standard sequence (2DPC MRI).

Furthermore, we expected that 4D flow MRI would permit an accurate diagnosis and provide additional quantitative and qualitative data not obtainable with 2DPC MRI, thus providing a more precise analysis of blood flow, especially in cases with non-laminar or eccentric flow.

Conclusions

The findings of this study confirm the value of 4D flow MRI for grading AR in clinical practice. Measures of blood flow and RF obtained with this technique do not differ from those obtained with 2DPC MRI. The present study adds to the growing body of evidence to support the clinical use of 4D flow MRI, which is likely to be included in routine clinical practice in the near future. Indeed, given the numerous advantages of this technique, it may even eventually replace 2DPC MRI in clinical practice. However, further studies are needed to optimise the clinical use of 4D flow MRI.