Fig. 3.

Haematopoiesis-regulating genes, which were downregulated by Beclin-1 knockdown, were upregulated by Beclin-1 overexpression in HUVECs. (a) Volcano plots of the differentially expressed genes between the H-sBECN and control groups. Red dots represent upregulated genes with log₂[H-sBECN/control]>0.5 and P<0.05. Blue dots represent downregulated genes with log₂[H-sBECN/control]<−0.5 and P<0.05. Grey dots represent genes with no significant difference between the H-sBECN and control groups. (b) Bar charts show the biological processes of the enriched downregulated genes in the H-sBECN group. The bar indicates the -log10 of the P-value. (c) The relative mRNA levels of Beclin-1, JAG1, CSF-1, CSF-2, CSF-3, ETS1, IL-7 and DLL1 in the H-sBECN and control groups were determined using qRT‐PCR. (d) The relative mRNA levels of Beclin-1, JAG1, CSF-1, CSF-2, CSF-3, ETS1, IL-7 and DLL1 in the H-sBECN, H-sB/pB and control groups were determined using qRT‐PCR.