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. 2019 Dec 6;16(1):14–26. doi: 10.1080/15476278.2019.1697597

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — a-e: TPLSM images of CD31-stained fibrin gels of HUVECs co-cultured with HDFs (a), HNFs (b), WJ MSCs (c), AD MSCs (d) and BM MSCs (e) after 14 days of cultivation at 37°C and 5% CO₂. Scale bar: 50 µm. Statistical evaluation of the four parameters: area of the vascular structures (f), structure volume (g), length of the structures (h) and number of branching points (i) in the structures .The statistical significance is provided with * shows the significance of the individual values with respect to the positive control. ELISA results with eight angiogenesis cytokines: TNF-α, IGF-1, VEGF, IL-6, bFGF, TGF-ß, EGF and Leptin (j). The absorbance was measured at a wavelength of 450 nm.