Figure 1.

MiR-1275 is overexpressed in LUAD and correlates with tumor progression and poor survival in LUAD patients. (A) Heat map showing 8 top upregulated miRNAs in the microarray (fold change ≥2, P<0.05). (B) MiR-1275 expression level in the miRNA microarray. (C) RT-qPCR analysis of miR-1275 expression in 8 pairs of LUAD tissues and adjacent nontumor tissues. (D) Expression of miR-1275 in normal lung epithelial cell line (BEAS2B) and lung cancer cell lines (n=9). (E) Representative images of ISH showing miR-1275 expression level in tumor tissues and adjacent nontumor tissues. U6, predominately localized in the nucleus, was used as the internal control. miR-1275 is abundantly localized in the membrane, cytoplasm, and nucleus of the tumor cells. The red arrows indicate the nuclei, and the green arrows indicate the cytoplasm and membranes. Scale bar, 50 μm. (F-H) Kaplan-Meier survival analysis of the correlation between miR-1275 expression and the OS of patients in all disease stages (stages I-IV) (F), early stages (stages I-II) (G) and the advanced stages (stages III-IV) (H). (I) Representative images of CTCs in LUAD patients (n=43); CTC numbers were positively correlated with miR-1275 expression. (J-K) Relative CD133 (J) and ALDH1 (K) expression levels in the miR-1275^low and miR-1275^high expression groups, respectively. Each experiment was performed three times. Data are shown as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001. ANT: adjacent nontumor tissues, T: tumor.