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. 2020 Feb 10;10(7):3325–3339. doi: 10.7150/thno.41228

Figure 3.

Figure 3

Gene silencing efficacy and cytotoxicity evaluation. Quantitative RT-PCR (A) and western blot (B) determination of E7 levels in HeLa cells treated with different siRNA-E7-based formulations at a constant siRNA concentration of 100 nM. Free siRNA-E7 and formulations containing scrambled siRNA were used as negative controls, lipofectamine 2000 was used as a positive control. (C) Cell viability of HeLa cells when treated with various formulations at indicated PTX concentrations and fixed siRNA concentrations (100 nM) for 48 h. (D) Flow cytometry analysis of HeLa cell apoptosis induced by SiNPs, free PTX, PNPs, PNPs@HeLa, NC/PNP@HeLa, Si/PNPs, and Si/PNPs@HeLa at a PTX concentration of 10 nM and an siRNA concentration of 100 nM for 48 h. (E) Corresponding statistics on the proportion of apoptotic cells. (F) Alteration of the pathways in the HeLa cells when treated for indicated times with 10 nM PTX. (G) The effects of Si/PNPs and Si/PNPs@HeLa on PTX-induced alteration of pathways in the HeLa cells, as detected by western blotting. Data are given as the mean ± SD (n=3). *p < 0.05, **p, < 0.01, ***p <0.001.