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. 2020 Mar 3;9:e52420. doi: 10.7554/eLife.52420

Figure 2. Dynamic baseline expression of sGluA1 within individual spines.

(A) Heat map of sGluA1 expression within individual spines. Spines on the same dendrites were grouped together. (B) Correlation of spine sGluA1 level between day 1 and day 10 in V1 L2/3 neurons (n = 279 spines; Pearson). (C) Correlation between spine sGluA1 level at day 1 and its CV in V1 L2/3 neurons (n = 280 spines; Pearson). (D) The CV of spine sGluA1 signal in V1 L2/3 neurons before and after visual deprivation (VD) (n = 280/520 (Sham/VD) spines in V1 L2/3 neurons; Student’s t-test). (E) Representative images of newly-formed spines (bottom) and eliminated spines (upper). Scale bars: 2 μm. (F) sGluA1 levels in newly-formed spines and eliminated spines (n = 29 spines; Student’s t-test). (G) The CV of spine sGluA1 expression in persistent and transient spines in V1 L2/3 neurons (n = 327 persistent spines; n = 34 transient spines). (H) Correlation between spine size and spine sGluA1 intensity on imaging day 1 (n = 280 spines; Pearson). (I) Correlation between daily change in spine size and daily change in spine sGluA1 level (n = 1384 spines; Pearson). Data are presented as mean ± SEM. n.s., not significant; *p<0.05; **p<0.01; ****p<0.0001.

Figure 2.

Figure 2—figure supplement 1. Dynamic sGluA1 expression within individual spines.

Figure 2—figure supplement 1.

(A) Gradual increases of sGluA1 level in newly-formed spines after they were first detected on Dn (n = 16 spines; Student’s t-test); (B) Gradual decreases of sGluA1 level in eliminated spines in days prior to elimination (Dn) (n = 19 spines; Student’s t-test). Blue, average. Grey, individual spines. (C) Heat map of change in spine size within individual spines. (D) Correlation of spine size between day 1 and day 10 in V1 L2/3 neurons (n = 278 spines; Pearson). (E) Correlation between daily change in spine size and daily change in spine sGluA1 level in the subset of spines that have inverse correlations between daily spine size change and sGluA1 change (n = 246 spines; Pearson). (F) Percentage of spines that show an inverse correlation between daily spine size change and spine sGluA1 change (17.77%). (G) Spine sGluA1 level distribution of all spines and spines that have inverse correlations between daily spine size change and sGluA1 change (Invers) (Kolmogorov-Smirnov test, p=0.0013). Data are presented as mean ± SEM. *p<0.05; **p<0.01.