Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Antibody	Anti-dsRed2 (rabbit polyclonal)	Clontech	Cat# 632496, RRID:AB_10013483	1:1000
Antibody	Anti-GFP (chicken polyclonal)	Abcam	Cat# ab13970, RRID:AB_300798	1:1000
Antibody	Alexa Fluor 568 goat anti-rabbit	Thermo Fisher Scientific	Cat# A-11011, RRID:AB_143157	1:500
Antibody	Alexa Fluor 488 goat anti-chicken	Thermo Fisher Scientific	Cat# A-11039, RRID:AB_2534096	1:500
Antibody	Anti-GluA1 (JH4294)	(Oku and Huganir, 2013)	N/A
Antibody	Anti-GluN1 (JH2590)	(Liao et al., 1999)	N/A
Chemical compound, drug	MNI-caged-L-glutamate	Tocris	Cat#0218, N/A
Strain, strain background	Mouse: WT C57BL/6N	Charles River	Strain #027, RRID:IMSR_CRL:027
Strain, strain background	Nestin-Grip1 fl/fl mouse	(Takamiya et al., 2008)	N/A
Recombinant DNA reagent	SEP-GluA1	(Zhang et al., 2015)	N/A
Recombinant DNA reagent	Myc-GluA2	(Zhang et al., 2015)	N/A
Recombinant DNA reagent	DsRed2	Clontech	Cat# 632405
Software, algorithms	MATLAB	Mathworks	https://www.mathworks.com,RRID:SCR_001622
Software, algorithms	ScanImage	(Pologruto et al., 2003)	https://www.vidriotechnologies.com, RRID:SCR_014307
Software, algorithms	IGOR Pro	WaveMetrics	https://www.wavemetrics.com /products/igorpro, RRID:SCR_000325
Software, algorithms	MapManager	(Zhang et al., 2015)	https://mapmanager.net/
Software, algorithms	ImageJ	(Schneider et al., 2012)	https://imageJ.net/, RRID:SCR_003070