Abstract
Background
Progression after high-dose melphalan with autologous stem cell transplantation (ASCT) in multiple myeloma (MM) may be due in part to immune dysfunction. Regulatory T (Treg) cells reconstitute rapidly after ASCT and inhibit immune responses against myeloma cells.
Methods
We performed a randomized study to evaluate two methods of Treg depletion in patients with MM undergoing ASCT. No Treg depletion was performed in the control ASCT arm. An anti-CD25 monoclonal antibody (basiliximab 20 mg IV) was administered on day +1 post-ASCT in the in vivo Treg depletion (IVTRD) arm. Tregs were depleted from autologous stem cell (ASC) grafts with anti-CD25 microbeads and the CliniMACS device in the ex vivo Treg depletion (EVTRD) arm.
Results
Fifteen patients were enrolled, five in each arm. The conditioning regimen was melphalan 200 mg/m2. Primary objectives included assessments of efficiency of IVTRD/EVTRD, kinetics of Treg depletion and recovery following ASCT, and safety. EVTRD removed 90% of CD4+CD25+ cells from ASC grafts. IVTRD and EVTRD led to reductions in Treg frequency between days +7 and +90 post-transplant compared with the control (p=0.007 and p<0.001, respectively).
Conclusions
IVTRD and EVTRD are feasible and significantly reduce and delay Treg recovery post-ASCT for MM, and serve as a platform for using post-transplant immunotherapies to improve post-ASCT outcomes.
Trial registration number
Keywords: autologous stem cell transplant, regulatory T cells, multiple myeloma, regulatory T cell depletion
Background
High-dose melphalan followed by autologous stem cell transplantation (ASCT) is a mainstay of intensification therapy for eligible patients with multiple myeloma (MM). When using bortezomib, lenalidomide, and dexamethasone as part of frontline therapy in the IFM-2009 study, early ASCT was shown to increase the depth of response and median progression-free survival (PFS) (50 vs 36 months, p<0.001) as compared with a strategy of delayed ASCT; at 4 years of follow-up, overall survival (OS) was similar between the two arms.1 These findings are in keeping with several other randomized phase III trials comparing ASCT to conventional chemotherapy prior to the inclusion of contemporary induction agents.2–7 In total, this indicates that even with modern induction and maintenance therapy, median PFS for post-transplant patients with MM is still under 5 years, which may be due to persistent minimal residual disease (MRD) following ASCT.
Immunotherapy is a non-cross-resistant therapeutic approach that may be most effective in this MRD-positive state. Regulatory T (Treg) cells represent a small but important subset of naturally suppressive CD4+ T cells (CD4+CD25+FoxP3+) that inhibit anticancer immune responses, thereby promoting tumor progression.8 9 High-dose chemotherapy followed by ASCT leads to protracted lymphopenia, which is then followed by expansion of reinfused T cells in the autologous stem cell (ASC) graft.10 11 Immune reconstitution of Tregs occurs as early as 2 weeks after ASCT, and Tregs remain elevated at day +90 before returning to normal levels approximately 6 months post-ASCT.11 12 Though the thymus represents the source for endogenous long-lived Treg cells, Treg expansion post-ASCT is likely coming from infused graft rather than the thymus. This suggests that there may be a short window in the post-ASCT period where Treg depletion may enhance the antitumor immune response in the MRD-positive state that exists following ASCT.
Cancers activate escape pathways in order to escape immune surveillance. Expansion and accumulation of Treg cells in the tumor microenvironment is one major immune evasion mechanism activated across human cancers, and MM is no exception.13–15 Tregs appear to play a significant role in MM progression, and Treg depletion in murine models has been effective in inhibiting MM progression through enhancing antimyeloma immune responses.16–18 However, there is a paucity of data on the ability to deplete Tregs and its efficacy in humans with MM.
To address the hypothesis that early Treg depletion can be accomplished in the post-ASCT MM setting, we conducted a randomized pilot study to evaluate the feasibility and efficacy of in vivo Treg depletion (IVTRD) and ex vivo Treg depletion (EVTRD) in patients with MM undergoing ASCT.
Methods
Criteria for enrollment
Transplant-eligible patients aged 21–70 years with symptomatic, newly diagnosed MM having undergone induction therapy were eligible for this study. Additional inclusion criteria were Eastern Cooperative Oncology Group (ECOG) performance status of ≤2, HIV and hepatitis B/C serology-negative, without cardiac or pulmonary dysfunction (left ventricular ejection fraction >50%, forced expiratory volume in one second (FEV1) >60%, and diffusing capacity of lung for carbon monoxide (DLCO) >60% predicted), bilirubin <2× Upper Limit of Normal, and estimated glomerular filtration rate >40 mL/min/1.73 m2. Exclusion criteria were pregnant or nursing women, use of systemic immunosuppressive medications, psychiatric illness, and active autoimmune disease (not including type 1 diabetes mellitus or autoimmune hypothyroidism). The protocol was registered as a randomized trial with ClinicalTrials.gov.
Trial design and treatment
Patients were enrolled from March 2013 through July 2017 and randomly assigned to one of three treatment groups: (1) no Treg depletion (‘control ASCT arm’), (2) IVTRD with the anti-CD25 monoclonal antibody basiliximab (‘IVTRD arm’), and (3) EVTRD of ASC grafts using CD25 microbeads (‘EVTRD arm’).
Peripheral blood stem cells were mobilized using filgrastim and plerixafor in all patients. The conditioning regimen consisted of melphalan 200 mg/m2, which was administered as 100 mg/m2 on days −3 and −2 prior to stem cell infusion on day 0. In the EVTRD arm, ASC grafts were depleted of Tregs using anti-CD25 microbeads and the CliniMACS device (Miltenyi Biotec, GmbH) prior to infusion. Basiliximab (20 mg), an anti-CD25 monoclonal antibody, was administered intravenously on day +1 to patients in the IVTRD arm. Patients were allowed to receive post-ASCT consolidation and/or maintenance therapy starting at day +100 at the discretion of the treating physician.
Endpoints
The primary endpoints were efficiency of Treg depletion in the IVTRD and EVTRD arms, post-transplant kinetics of Treg depletion and recovery up to day +180, and clinical toxicities of these three arms. Secondary endpoints were the rate and timing of neutrophil and platelet engraftment, and disease response.
Assessments
Peripheral blood Tregs were identified as CD4+CD25+FoxP3+CD127lo cells by flow cytometry of peripheral blood (figure 1) and as CD4+CD25+ cells in ASC grafts (figure 2), as described previously.19 20 Peripheral blood samples were collected at days −3, 0, +7, +14, +21, +28, +42, +60 and +90 post-ASCT as prespecified timepoints for assessing Treg depletion. Day +180 was a prespecified timepoint, but only one patient had peripheral blood drawn at this timepoint due to low adherence, as 13/14 patients went on maintenance therapy prior to this timepoint, which was thought to likely confound results. Other T-cell subsets, such as central memory or effector memory T cells, were not analyzed.
Figure 1.
Flow cytometry gating strategy to identify peripheral blood Tregs. (A) Lymphocyte population was identified by forward scatter (FSC)/side scatter (SSC) gating. (B) Frequency of CD3+CD4+ T cells. (C) CD4+CD127lo T cells. (D) After gating on CD4+CD127lo cells, Treg cells were identified as CD25+FoxP3+ cells. Flow cytometry analysis was performed using MACSQuant analyzer (Miltenyi Biotec).
Figure 2.
EVTRD from an autologous stem cell graft using anti-CD25 microbeads and the CliniMACS device. The predepletion lymphocyte population is identified in (A), and relative CD4+CD25+/CD4+ T cells are identified in (B). Postdepletion lymphocyte population and relative CD4+CD25+/CD4+ T cells are identified in (C) and (D), respectively. in this example, the relative efficiency of EVTRD was approximately 98%. Flow cytometry analysis was performed using MACSQuant analyzer (Miltenyi Biotec). EVTRD, ex vivo Treg depletion; FITC, fluorescein isothiocyanate; FSC, forward scatter; SSC, side scatter.
Best treatment response and disease progression were evaluated according to the International Myeloma Working Group (IMWG) consensus criteria for response and MRD assessment in MM.21 MRD was assessed using multiparametric flow cytometry at a depth of at least 10−4 (range 10−4 to 10−5). PFS was defined as the time from the date of ASC infusion until the first documentation of disease progression or death from any cause. OS was defined as the time from the date of ASC infusion until death from any cause.
Continuous variables were compared using the Mann-Whitney U-test and the independent samples t-test for non-normally and normally distributed data, respectively. Categorical variables were compared using the Fisher’s exact test. A two-way analysis of variance (ANOVA) was performed to compare the main effects of treatment arm and post-transplant time and the interaction effect between treatment arm and post-transplant time on Treg frequency. Treatment arms included three groups (control ASCT, IVTRD, and EVTRD); post-transplant time consisted of seven timepoints (‘day +7’, ‘day +14’, ‘day +21’, ‘day +28’, ‘day +42’, ‘day +60’ and ‘day +90’). Analyses were performed using STATA software V.15.0. The cut-off date for analysis was January 15, 2019.
Results
Patient characteristics
A total of 15 patients were enrolled, 5 in each arm. One patient in the IVTRD arm was removed from study due to mobilization failure, leaving 14 patients available for evaluation.
Baseline patient characteristics are shown in table 1, and individual patient characteristics and responses are found in table 2. No significant differences in age, gender, race, or International Staging System stage were present among the three arms. Compared with the IVTRD group, the EVTRD arm had fewer patients with high-risk cytogenetics as defined by the IMWG (1/5 vs 4/4, p=0.048); the control ASCT arm had two of five patients with high-risk cytogenetics (p=not significant (NS)). Patients in all three cohorts received a median of 4 cycles of induction therapy, with the majority receiving triplet therapies (bortezomib, lenalidomide and dexamethasone or carfilzomib, lenalidomide, and dexamethasone). All patients achieved at least a partial response prior to ASCT. A very good partial response or better was achieved in two of fie patients in the control ASCT arm, three of four in the IVTRD arm, and two of five in the EVTRD arm (p=NS between groups). The median and range of the infused CD34+ cell dose (×106/kg) was 4.6 (range 3.16–6.03) in the control ASCT arm, 2.9 (range 2.65–10.76) in the IVTRD arm, and 3.5 (range 1.95–5.16) in the EVTRD arm (p=NS between groups).
Table 1.
Patient characteristics
| Control ASCT (n=5) | IVTRD (n=4) |
EVTRD (n=5) |
|
| Age (years), median | 60.3 | 64.0 | 57.1 |
| Male gender, n (%) | 2 (40) | 3 (75) | 2 (40) |
| Race/ethnicity, n (%) | |||
| Caucasian | 4 (80) | 4 (100) | 1 (20) |
| Black | 1 (20) | 0 (0) | 3 (60) |
| Hispanic/Latino | 0 (0) | 0 (0) | 1 (20) |
| ISS stage, median | 1 | 1 | 1 |
| 1 | 3 | 3 | 3 |
| 2 | 1 | 1 | 2 |
| 3 | 1 | 0 | 0 |
| High-risk cytogenetics, n (%) | 2 (40) | 4 (100) | 1 (20)* |
| Induction therapy, n (%) | |||
| RVd | 3 (60) | 3 (75) | 5 (100) |
| KRd | 1 (20) | 0 (0) | 0 (0) |
| Rd | 1 (20) | 0 (0) | 0 (0) |
| Vd | 0 (0) | 1 (25) | 0 (0) |
| Induction cycles. median | 4 | 4 | 4 |
| Best induction response, n (%) | |||
| PR | 3 (60) | 1 (25) | 3 (60) |
| VGPR | 1 (20) | 1 (25) | 1 (20) |
| CR | 0 (0) | 1 (25) | 0 (0) |
| sCR | 1 (20) | 1 (25) | 1 (20) |
| Stem cell dose (CD34+×106/kg), median (range) |
4.6 (2.4–6.5) | 2.9 (2.7–10.8) | 3.5 (2.0–5.2) |
*p<0.05 compared with the IVTRD arm.
ASCT, autologous stem cell transplant; CR, complete response; EVTRD, ex vivo regulatory T-cell depletion;ISS, International Staging System; IVTRD, in vivo regulatory T-cell depletion; KRd, carfilzomib, lenalidomide, and dexamethasone; PR, partial response; Rd, lenalidomide+dexamethasone; RVd, lenalidomide, bortezomib, and dexamethasone; sCR, stringent complete response; Vd, bortezomib+dexamethasone; VGPR, very good partial response.
Table 2.
Individual patient characteristics and responses
| Patient number | Age (years) | R-ISS | Induction regimen | Pre-ASCT response | Best post-ASCT response | Post-ASCT consolidation | Post-ASCT maintenance |
| Control | |||||||
| 1 | 64 | 2 | VRd | PR | PR | VRd×2 | None* |
| 2 | 50 | 2 | VRd | sCR | MRD-neg sCR | None | R |
| 3 | 60 | 2 | VRd | PR | PR | VRd×4 | VRd |
| 4 | 56 | 3 | Rd | VGPR | sCR | None | R |
| 5 | 71 | 2 | KRd | PR | MRD-neg sCR | None | R |
| IVTRD | |||||||
| 6 | 49 | 2 | VRd | PR | VGPR | None | R |
| 7 | 62 | 1 | Vd | MRD-neg sCR | sCR | VRd×2 | R |
| 8 | 65 | 1 | VRd | VGPR | MRD-neg sCR | VRd×4 | R |
| 9 | 65 | 1 | VRd | CR | sCR | None | R |
| EVTRD | |||||||
| 10 | 57 | N/A | VRd | PR | MRD-neg sCR | None | R |
| 11 | 46 | 1 | VRd | sCR | sCR | VRd×2 | R |
| 12 | 67 | 2 | VRd | PR | MRD-neg sCR | VRd×2 | R |
| 13 | 64 | 2 | VRd | VGPR | MRD-neg sCR | KRd×8 | R |
| 14 | 53 | 1 | VRd | PR | MRD-neg sCR | None | R |
*Patient declined maintenance therapy.
ASCT, autologous stem cell transplant; CR, complete response; EVTRD, ex vivo Treg depletion; IVTRD, in vivo Treg depletion;KRd, carfilzomib, lenalidomide, and dexamethasone; MRD-neg, minimal residual disease negative; PR, partial response; R, lenalidomide;Rd, lenalidomide+dexamethasone; R-ISS, Revised-International Staging System; sCR, stringent complete response;Vd, bortezomib+dexamethasone; VGPR, very good partial response; VRd, bortezomib, lenalidomide, and dexamethasone .
Efficiency of ex vivo Treg cell depletion
Prior to ASC graft manipulation, the median relative Treg frequency (CD4+CD25+/CD4+) was 8.3% (range 6.8%–19.2%). Following CD25+ cell separation from ASC grafts, the median relative Treg frequency was 0.9% (range 0.1%–2.9%, p=0.0049), corresponding to a 90% depletion of CD4+CD25+ T cells (figure 3).
Figure 3.
EVTRD from ASC grafts is highly efficient. Frequencies of CD4+CD25+ Treg cells in each ASC graft are shown prior to and following the EVTRD procedure (p=0.0049). ASC, autologous stem cell; EVTRD, ex vivo Treg depletion.
Kinetics of Treg cell recovery in the post-ASCT setting
Treg frequencies from peripheral blood patient samples at each point post-ASCT (CD4+CD25+FoxP3+CD127lo/CD4+) are shown in figure 4. Treg frequencies in both the IVTRD and EVTRD arm were generally lower than those in the control ASCT arm. A two-way ANOVA was performed to examine the effect of treatment arm and post-transplant time on Treg frequency. Simple main effects analysis revealed that the treatment arm had a significant effect on Treg frequency (F(2, 45)=8.35, p=0.008). The main effect of post-transplant time on Treg frequency was not significant (F(6, 45)=1.92, p=0.0977). The interaction effect of treatment arm and post-transplant time was not significant (F(12, 45)=0.49, p=0.9115). Using the Bonferroni correction as a post hoc exploratory analysis, IVTRD and EVTRD each individually led to significant reductions in Treg frequency between days +7 and +90 post-transplant compared with the control ASCT arm (p=0.007 and p<0.001, respectively). With the control ASCT group as the comparator in a prespecified analysis of individual timepoints, only the EVTRD arm had a significantly lower median Treg frequency at day +21 post-ASCT (p=0.025).
Figure 4.
Kinetics of Treg cell depletion and recovery following IVTRD and EVTRD. Median Treg frequencies by treatment arm at the indicated timepoints are shown. The asterisk (*) indicates p=0.025 for comparison of median Treg cell frequencies in the EVTRD versus control arm at day +21. Color-coded error bars represent the SE of the mean. ASCT, autologous stem cell transplantation; EVTRD, ex vivo Treg depletion; IVTRD, in vivo Treg depletion.
Patient outcomes
The median time to neutrophil and platelet engraftment was similar across all three arms (10–11 days). Febrile neutropenia occurred in four of five patients in the EVTRD arm, in one of four patients in the IVTRD arm, and two of five patients in the control ASCT arm (p=NS). Rash occurred in only one patient and involved 77% body surface area; this patient was in the EVTRD arm and received systemic corticosteroids, which led to prompt resolution of the rash. The Treg frequency at the time of symptoms was 0.4% compared with 2.4% prior to ASCT (83.3% decrease). The autograft for this patient had 98.2% of Tregs depleted. Diarrhea occurred in one patient in each arm (p=NS). The number of immune-related adverse events was too few to make any firm conclusions between degree of Treg depletion and the event. A complete list of nonhematological adverse events can be found in table 3. Post-ASCT consolidation and maintenance therapy were administered at similar rates across the groups. One patient in the EVTRD arm received a tandem ASCT, and one patient in the control arm did not receive maintenance therapy due to patient preference.
Table 3.
Non-hematological adverse events
| Event | Control ASCT (n=5) |
IVTRD ASCT (n=4) |
EVTRD ASCT (n=5) |
|||
| All grade |
Grade 3 or 4 |
All grade |
Grade 3 or 4 |
All grade | Grade 3 or 4 |
|
| Constitutional symptoms | ||||||
| Fatigue | 4 (80%) | 1 (20%) | 0 | 0 | 0 | 0 |
| Fever | 3 (60%) | 2 (40%) | 1 (25%) | 1 (25%) | 4 (80%) | 4 (80%) |
| Pain | 2 (40%) | 0 | 1 (25%) | 0 | 1 (20%) | 0 |
| Weight loss | 0 | 0 | 0 | 0 | 1 (20%) | 0 |
| Malaise | 0 | 0 | 0 | 0 | 1 (20%) | 0 |
| Gastrointestinal disorders | ||||||
| Nausea | 2 (40%) | 0 | 1 (25%) | 0 | 1 (20%) | 0 |
| Diarrhea | 1 (20%) | 0 | 1 (25%) | 0 | 1 (20%) | 0 |
| Hepatobiliary disorders | ||||||
| Increased aminotransferase level | 1 (20%) | 0 | 0 | 0 | 1 (20%) | 1 (20%) |
| Hyperbilirubinemia | 1 (20%) | 1 (20%) | 0 | 0 | 1 (20%) | 0 |
| Electrolyte disorders | ||||||
| Hypocalcemia | 2 (40%) | 0 | 0 | 0 | 1 (20%) | 0 |
| Hypokalemia | 1 (20%) | 0 | 1 (25%) | 0 | 2 (40%) | 1 (20%) |
| Hypophosphatemia | 1 (20%) | 0 | 1 (25%) | 0 | 1 (20%) | 1 (20%) |
| Miscellaneous | ||||||
| Hyperglycemia | 2 (40%) | 1 (20%) | 1 (25%) | 0 | 2 (40%) | 1 (20%) |
| Peripheral neuropathy | 1 (20%) | 0 | 1 (25%) | 0 | 0 | 0 |
| Chronic kidney disease | 1 (20%) | 0 | 1 (25%) | 0 | 0 | 0 |
| Bone fracture | 0 | 0 | 1 (25%) | 1 (25%) | 0 | 0 |
| Rash | 0 | 0 | 0 | 0 | 1 (20%) | 1 (20%) |
| Cough | 0 | 0 | 0 | 0 | 1 (20%) | 0 |
| Lymphadenitis | 0 | 0 | 0 | 0 | 1 (20%) | 0 |
| Hemorrhoids | 1 (20%) | 0 | 0 | 0 | 0 | 0 |
Shown are adverse events (irrespective of the relationship to the study drugs) that were reported in at least one patient.
ASCT, autologous stem cell transplant; EVTRD, ex vivo regulatory T-cell depletion; IVTRD, in vivo regulatory T-cell depletion.
The median follow-up time was 59, 36, and 68 months for the control ASCT, IVTRD, and EVTRD arms, respectively. All five patients in the EVTRD arm achieved a stringent CR, and four of five achieved MRD negativity by flow cytometry (depth 10−4 to 10−5). In the IVTRD arm, three of four patients achieved an sCR (one was MRD-negative). In the control ASCT arm, three of five patients achieved a sCR with two MRD-negative responses (table 4).
Table 4.
Patient outcomes
| Control ASCT (n=5) | IVTRD ASCT (n=4) | EVTRD ASCT (n=5) | |
| Days to neutrophil engraftment, median (range) | 11 (10–12) | 11 (11–13) | 10 (10–11) |
| Days to platelet engraftment, median (range) | 10 (10–11) | 10.5 (9–12) | 11 (10–14) |
| Grade 3/4 infections, n (%) | 2 (40) | 2 (50) | 4 (80) |
| Engraftment syndrome, n (%) | 0 (0) | 0 (0) | 1 (20) |
| Received consolidation, n (%) | 2 (40) | 2 (50) | 3 (60) |
| Received maintenance, n (%) | 4 (80) | 4 (100) | 5 (100) |
| Best response prior to ASCT, n (%) | |||
| PR | 3 (60) | 1 (25) | 3 (60) |
| VGPR | 1 (20) | 1 (25) | 1 (20) |
| CR | 0 (0) | 1 (25) | 0 (0) |
| sCR | 1 (20) | 0 (0) | 1 (20) |
| sCR+flow MRD-negative | 0 (0) | 1 (25) | 0 (0) |
| Best response following ASCT, n (%) | |||
| PR | 2 (40) | 0 (0) | 0 (0) |
| VGPR | 0 (0) | 1 (25) | 0 (0) |
| CR | 0 (0) | 0 (0) | 0 (0) |
| sCR | 1 (20) | 2 (50) | 1 (20) |
| sCR+flow MRD-negative | 2 (40) | 1 (25) | 4 (80) |
ASCT, autologous stem cell transplant;CR, complete response; EVTRD, ex vivo regulatory T-cell depletion; IVTRD, in vivo regulatory T-cell depletion; MRD, minimal residual disease (depth between 10−4 and 10−5); PR, partial response; sCR, stringent complete response; VGPR, very good partial response.
Disease relapse occurred in five patients (two in the control ASCT arm, two in the IVTRD arm, and one in the EVTRD arm). Death occurred in two patients, ultimately due to disease progression: one in the control ASCT arm due to liver failure and one in the IVTRD arm due to metabolic encephalopathy. There were late effects deemed unrelated to study treatment. The 3-year PFS rate was 80% in the control ASCT arm and 100% in both the IVTRD and EVTRD arms; the 3-year OS rate was 100% in all arms. One patient in the EVTRD arm developed therapy-related acute myeloid leukemia.
Discussion
Inhibiting Treg cell recovery following ASCT in MM is a unique immunotherapeutic approach to potentially enhance MM-specific immune responses in the MRD-positive state. Previous data have suggested that Tregs not only reconstitute early following ASC infusion but also appear to expand in the early post-ASCT period and return to normal levels by 6 months.11 12 Indeed, this points to a narrow window in the post-ASCT period when Treg depletion may be effective in enhancing antitumor immune responses. Despite the success in Treg depletion in murine models, there has been only one prior study using denileukin difitox to accomplish the same in humans and was closed after enrollment of only two patients due to drug discontinuation by the manufacturer. Mogamulizumab, an anti-CC4 monoclonal antibody, appears to reduce CCR4+ Treg cells in cutaneous T-cell lymphoma but has not yet been proven in the setting of MM.22 23
Our results clearly demonstrate that both EVTRD of ASC grafts and IVTRD using basiliximab are efficient and feasible in patients with MM and importantly lead to significant reductions in Tregs in the early post-ASCT period in MM. In an exploratory analysis of Treg frequencies between days +7 and +90, post-ASCT Tregs were significantly reduced in the IVTRD and EVTRD arms compared with the control arm. This was achieved without negatively impacting engraftment, and without significant autoimmune complications, such as the development of autologous graft-versus-host disease.
While the EVTRD of ASC grafts was feasible and effective at reducing the numbers of Tregs in the early post-transplant period, it is unknown how efficient basiliximab at the dose administered was with respect to IVTRD in the context of this study. The half-life of basiliximab is reported to be 7.2±2.3 days and is not influenced by age, gender, or race24; this would suggest that a single dose should be sufficient to achieve Treg depletion within the first 30–40 days post-ASCT. Whether a second dose of basiliximab post-transplant might enhance the effect of Treg depletion might be the subject of further investigation.
The primary endpoints of this study were with respect to the kinetics of Tregs in the post-transplant period; outcomes represented secondary endpoints that were not the main focus of this study. Moreover, in light of the small sample size in this trial, the clinical outcome data must be interpreted with caution. Though this was a randomized pilot study, patients were not stratified by cytogenetic risk or by induction regimen. As such, the difference in baseline high-risk cytogenetics or induction regimens used between arms may account for differences in response. Nevertheless, there appeared to be a signal that EVTRD led to deeper responses, which is possibly explained by an enhancement of a post-transplant antitumor response.
Treg cell depletion could serve as a non-cross-resistant therapeutic approach in the MRD-positive state and should be investigated as a platform on which other post-transplant immunotherapies could be administered to improve post-ASCT outcomes. In particular, we posit that EVTRD should be the basis for a post-ASCT combinatory immunotherapeutic approach given its reliability in efficient Treg depletion. There are already several effective uses of immunotherapies in MM, and other promising modalities are under way. Monoclonal antibodies daratumumab and elotuzumab are Food and Drug Administration (FDA)-approved for relapsed/refractory MM, with daratumumab now approved for frontline use as well. Checkpoint inhibitors targeting the PD-1/PD-L1 pathway have been an active area of interest, particularly in the early post-transplant period; however, studies involving pembrolizumab were halted by the FDA due to an increased risk of death.25 Chimeric antigen receptor T-cell (CAR-T) therapy has shown to be effective in patients with multiply relapsed MM,26 and there are several competing CAR-T constructs that are currently under investigation. Recent work has exhibited the safety and efficacy of autologous New York esophageal squamous cell carcinoma-1 (NY-ESO-1)-specific peptide enhanced affinity receptor (SPEAR) T cells in the post-ASCT period (when presented by human leukocyte antigenHLA-A*02:01).27 Combination of Treg depletion and adoptive transfer of NY-ESO-1 SPEAR T cells represents another promising pairing to enhance antitumor activity post-ASCT in MM.
Conclusions
Immunotherapeutic approaches may be effective in eliminating MRD, particularly when combined with ASCT. We have shown that Treg depletion in the early post-transplant period is a feasible and safe immunotherapeutic approach in MM. Further investigation of the interplay between Treg depletion with other forms of immunotherapy in the early post-transplant period is warranted.
Acknowledgments
The authors thank the patients and their families, investigators, study coordinators, and support staff who played a role in conducting this study.
Footnotes
Twitter: @bdermanmd
Contributors: JK designed the study; BAD, YZ, RM, AJJ, TMZ, MRB, and JK collected and assembled data; BAD, YZ, AJJ, MRB, and JK analyzed and interpreted the data. BAD, MRB, and JK wrote the manuscript; and all authors participated in manuscript development and final approval.
Funding: This research was supported by National Cancer Institute grant K23 CA133196.
Competing interests: AJJ serves on the advisory boards of Amgen, Bristol-Myers-Squibb, Celgene, Janssen, Takeda, and SkylineDX, and serves as a consultant for Abbvie. TMZ is an employee of Abbvie. MRB serves on the advisory boards of Celgene, CRISPR Therapeutics, Juno, Kite Pharma, Pharmacyclics, Seattle Genetics, and United Healthcare and serves as a consultant for Kite Pharma. JK receives honoraria from Kite, Merck, and Seattle Genetics, and receives research funding from iTeos and Merck.
Patient consent for publication: Not required.
Ethics approval: The protocol was approved by the institutional review board (IRB) of the University of Chicago Medical Center (IRB #10-551-B). All patients provided written inform consent.
Provenance and peer review: Not commissioned; externally peer reviewed.
Data availability statement: Data are available upon reasonable request. All data relevant to the study are included in the article or uploaded as supplementary information.
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