Figure 3.

TCI inhibitory effect is not significantly recovered by access amount of primer–template (P/T). All reaction mixtures contained P/T and HIV RT in the reaction buffer. In addition, samples 1 and 2 contained azidothymidine (AZT), while samples 3 and 4 contained both 6Az-dTTP and DBCO-dTTP. The samples were incubated for 45 min at 37 °C. Samples 2 and 4 were then treated with 1 μM P/T (to double its concentration), while all samples were treated with the dNTP mixture (100 μM dATP, 100 μM dTTP, 100 μM dCTP, 10 μM [8-H³]-dGTP) followed by incubation for 20 min at 37 °C. All reactions were quenched by the addition of EDTA (0.5 M) and transferred to DE 81 paper for quantification by a filter binding assay (see the Supporting Information for details). The data of three independent experiments with single standard deviations are presented.