Figure 3. Quantification of vesicle dynamics in the TIRF zone.

Counts of mobile and stationary vesicles for 3T3-L1 adipocytes (A) and HeLa cells (B) stimulated by 100 nM insulin were determined as outlined in “Materials and Methods”. A total of 10 individual 100 µm² regions of interest were analysed from three cells from three separate platings of cells. Error bars correspond to standard deviation for 30 measured ROIs. Images were recorded at a frame rate of 2 Hz for 15 min where time point 0 corresponds to point of insulin addition.