Table 1.
Isolates and accession numbers of sequences used in the phylogenetic analyses.
| Taxon | Strain1 | Origin | Host | GenBank accession no.2 | |||||
|---|---|---|---|---|---|---|---|---|---|
| ITS | LSU | ms204 | rpb2 | tef1 | tub2 | ||||
| Juglanconis appendiculata | MC | Greece | Juglans regia | KY427141 | KY427141 | KY427159 | KY427191 | KY427210 | KY427227 |
| Juglanconis japonica | MAFF 410079 = ME20* | Japan | Pterocarya rhoifolia | KY427155 | KY427155 | KY427172 | KY427205 | KY427224 | KY427240 |
| Juglanconis juglandina | CBS 133343 = ME22 | Austria | Juglans regia | KY427149 | KY427149 | KY427166 | KY427199 | KY427218 | KY427234 |
| Juglanconis oblonga | CBS 133344 = ME14 | USA | Juglans cinerea | KY427151 | KY427151 | KY427168 | KY427201 | KY427220 | KY427236 |
| Juglanconis pterocaryae | CBS 144326 = D272* | Austria | Pterocarya fraxinifolia | MK229175 | MK229175 | MK238314 | MK238324 | MK238332 | MK238338 |
| Melanconis alni | CBS 131693 = MAMI | Austria | Alnus glutinosa | MN784962 | MN784962 | MN780721 | MN780745 | MN780774 | MN780803 |
| CBS 131695 = MAW* (from ascospores) | Austria | Alnus glutinosa | MN784963 | MN784963 | MN780722 | MN780746 | MN780775 | MN780804 | |
| MEW*(from conidia) | Austria | Alnus glutinosa | MN784964 | MN784964 | MN780723 | MN780747 | MN780776 | MN780805 | |
| MAIV | France | Alnus incana | MN784965 | MN784965 | MN780724 | MN780748 | MN780777 | MN780806 | |
| D156 | Poland | Alnus glutinosa | MN784966 | MN784966 | MN780725 | MN780749 | MN780778 | MN780807 | |
| Melanconis betulae | CFCC 50471* | China | Betula albosinensis | KT732952 | KT732971 | – | KT732984 | KT733001 | KT733022 |
| CFCC 50472 | China | Betula albosinensis | KT732953 | KT732972 | – | KT732985 | KT733002 | KT733023 | |
| CFCC 50473 | China | Betula albosinensis | KT732954 | KT732973 | – | KT732986 | KT733003 | KT733024 | |
| Melanconis groenlandica | CBS 116450 = UPSC 3407* | Denmark (Greenland) | Betula nana | KU878552 | KU878553 | – | – | KU878554 | KU878555 |
| MAFF 410219 = M4-2 = ME1 | Japan | Betula maximowicziana | MN784967 | MN784967 | MN780726 | MN780750 | MN780779 | MN780808 | |
| CBS 133341 = LCM191.01 = ME10 | USA | Betula papyrifera | MN784968 | MN784968 | MN780727 | MN780751 | MN780780 | MN780809 | |
| CBS 133339 = LCM 02.02 = ME13 | USA | Betula sp. | MN784969 | MN784969 | MN780728 | MN780752 | MN780781 | MN780810 | |
| CBS 133340 = LCM 185.01 | USA | Betula papyrifera | MN784970 | MN784970 | MN780729 | MN780753 | MN780782 | MN780811 | |
| Melanconis itoana | MAFF 410080 = LFP-M4-9 = ME8 | Japan | Betula ermanii | MN784971 | MN784971 | MN780730 | MN780754 | MN780783 | MN780812 |
| CFCC 50474 | China | Betula albosinensis | KT732955 | KT732974 | – | KT732987 | KT733004 | KT733025 | |
| CFCC 52876 | China | Betula albosinensis | MK096324 | MK096364 | – | MK096409 | MK096284 | – | |
| CFCC 52877 | China | Betula albosinensis | MK096326 | MK096366 | – | MK096411 | MK096286 | – | |
| CFCC 52878 | China | Betula albosinensis | MK096327 | MK096367 | – | MK096412 | MK096287 | – | |
| Melanconis larissae | CBS 123196 = AR 3886 = ME7* | USA | Betula sp. | MN784972 | MN784972 | MN780731 | MN780755 | MN780784 | MN780813 |
| Melanconis marginalis subsp. europaea | D157 | Austria | Alnus alnobetula | MN784973 | MN784973 | – | MN780756 | MN780785 | – |
| D158 | Austria | Alnus alnobetula | MN784974 | MN784974 | MN780732 | MN780757 | MN780786 | MN780814 | |
| D257 | Austria | Alnus incana | MN784975 | MN784975 | – | MN780758 | MN780787 | MN780815 | |
| CBS 131692 = MAI* | Austria | Alnus incana | MN784976 | MN784976 | MN780733 | MN780759 | MN780788 | MN780816 | |
| CBS 131694 = MAV | Austria | Alnus alnobetula | MN784977 | MN784977 | MN780734 | MN780760 | MN780789 | MN780817 | |
| MAV1 | Austria | Alnus alnobetula | MN784978 | MN784978 | MN780735 | MN780761 | MN780790 | MN780818 | |
| Melanconis marginalis subsp. italica | MFLUCC 16-1199* | Italy | Alnus cordata | MF190151 | MF190096 | – | – | – | – |
| MFLUCC 17-1659* | Italy | Alnus cordata | MF190152 | MF190097 | – | MF377602 | – | – | |
| Melanconis marginalis subsp. marginalis | D321 (from ascospores)* | Canada | Alnus alnobetula subsp. crispa | MN784979 | MN784979 | – | MN780762 | MN780791 | MN780819 |
| D321a (from α-conidia)* | Canada | Alnus alnobetula subsp. crispa | MN784980 | MN784980 | – | MN780763 | MN780792 | MN780820 | |
| D321b (from β-conidia)* | Canada | Alnus alnobetula subsp. crispa | MN784981 | MN784981 | – | MN780764 | MN780793 | MN780821 | |
| CBS 109496 = AR 3529 = ME2 | Russia | Alnus alnobetula subsp. maximowiczii | MN784982 | MN784982 | MN780736 | MN780765 | MN780794 | MN780822 | |
| AR 4864 = ME5 | USA | Alnus alnobetula | MN784983 | MN784983 | MN780737 | MN780766 | MN780795 | MN780823 | |
| CBS 133346 = AR 4865 = ME6 | USA | Alnus alnobetula | MN784984 | MN784984 | MN780738 | MN780767 | MN780796 | MN780824 | |
| MAFF 410218 = M4-6 = ME9 | Japan | Alnus alnobetula subsp. maximowiczii | MN784985 | MN784985 | MN780739 | MN780768 | MN780797 | MN780825 | |
| Melanconis marginalis subsp. tirolensis | CBS 122310 = AR 3748 = ME4* | Austria | Alnus alnobetula | MN784986 | MN784986 | MN780740 | MN780769 | MN780798 | MN780826 |
| D322a | Austria | Alnus alnobetula | MN959458 | MN959458 | – | MN989415 | MN989416 | MN989417 | |
| Melanconis pacifica | CBS 109744 = AR 3442 = AFTOL-ID 2128 | Canada | Alnus rubra | EU199197 | AF408373 | – | DQ862022 | DQ862038 | EU219103, DQ862038 |
| Melanconis stilbostoma | D143 | Poland | Betula pendula | KY427156 | KY427156 | KY427173 | KY427206 | KY427225 | KY427241 |
| D258 | Italy | Betula aetnensis | MN784987 | MN784987 | – | MN780770 | MN780799 | MN780827 | |
| CBS 109778 = AR 3501 = AFTOL-ID 936 = ME11* | Austria | Betula pendula | MN784988 | MN784988 | MN780741 | MN780771 | MN780800 | MN780828 | |
| MAFF 410225 = M3-9 = ME12 | Japan | Betula platyphylla var. japonica | MN784989 | MN784989 | MN780742 | MN780772 | MN780801 | MN780829 | |
| CBS 121894 = MS | Austria | Betula pendula | KY427156 | KY427156 | MN780743 | JQ926302 | JQ926368 | MN780830 | |
| CBS 133338 = DMW 514.3 | USA | Betula papyrifera | MN784990 | MN784990 | MN780744 | MN780773 | MN780802 | MN780831 | |
| CFCC 50475 | China | Betula platyphylla | KT732956 | KT732975 | – | KT732988 | KT733005 | KT733026 | |
| CFCC 50476 | China | Betula platyphylla | KT732957 | KT732976 | – | KT732989 | KT733006 | KT733027 | |
| CFCC 50477 | China | Betula platyphylla | KT732958 | KT732977 | – | KT732990 | KT733007 | KT733028 | |
| CFCC 50478 | China | Betula platyphylla | KT732959 | KT732978 | – | KT732991 | KT733008 | KT733029 | |
| CFCC 50479 | China | Betula platyphylla | KT732960 | KT732979 | – | KT732992 | KT733009 | KT733030 | |
| CFCC 50480 | China | Betula platyphylla | KT732961 | KT732980 | – | KT732993 | KT733010 | KT733031 | |
| CFCC 50481 | China | Betula platyphylla | KT732962 | KT732981 | – | KT732994 | KT733011 | KT733032 | |
| CFCC 50482 | China | Betula platyphylla | KT732963 | KT732982 | – | KT732995 | KT733012 | KT733033 | |
| CFCC 50483 | China | Betula platyphylla | KT732964 | KT732983 | – | KT732996 | KT733013 | KT733034 | |
| CFCC 52843 | China | Betula platyphylla | MK096338 | MK096378 | – | MK096423 | MK096298 | – | |
| CFCC 52844 | China | Betula platyphylla | MK096341 | MK096381 | – | MK096426 | MK096301 | – | |
| CFCC 52845 | China | Betula platyphylla | MK096343 | MK096383 | – | MK096428 | MK096303 | – | |
| Melanconis stilbostoma | CFCC 52846 | China | Betula platyphylla | MK096347 | MK096387 | – | MK096432 | MK096307 | – |
| CFCC 52847 | China | Betula platyphylla | MK096348 | MK096388 | – | MK096433 | MK096308 | – | |
| CFCC 52848 | China | Betula platyphylla | MK096349 | MK096389 | – | MK096434 | MK096309 | – | |
| CFCC 52849 | China | Betula platyphylla | MK096328 | MK096368 | – | MK096413 | MK096288 | – | |
| CFCC 52850 | China | Betula platyphylla | MK096329 | MK096369 | – | MK096414 | MK096289 | – | |
| CFCC 52851 | China | Betula platyphylla | MK096330 | MK096370 | – | MK096415 | MK096290 | – | |
| CFCC 52852 | China | Betula platyphylla | MK096331 | MK096371 | – | MK096416 | MK096291 | – | |
| CFCC 52853 | China | Betula platyphylla | MK096332 | MK096372 | – | MK096417 | MK096292 | – | |
| CFCC 52854 | China | Betula platyphylla | MK096333 | MK096373 | – | MK096418 | MK096293 | – | |
| CFCC 52855 | China | Betula platyphylla | MK096334 | MK096374 | – | MK096419 | MK096294 | – | |
| CFCC 52856 | China | Betula platyphylla | MK096335 | MK096375 | – | MK096420 | MK096295 | – | |
| CFCC 52857 | China | Betula platyphylla | MK096336 | MK096376 | – | MK096421 | MK096296 | – | |
| CFCC 52858 | China | Betula platyphylla | MK096337 | MK096377 | – | MK096422 | MK096297 | – | |
| CFCC 52859 | China | Betula platyphylla | MK096339 | MK096379 | – | MK096424 | MK096299 | – | |
| CFCC 52860 | China | Betula platyphylla | MK096340 | MK096380 | – | MK096425 | MK096300 | – | |
| CFCC 52861 | China | Betula platyphylla | MK096342 | MK096382 | – | MK096427 | MK096302 | – | |
| CFCC 52862 | China | Betula platyphylla | MK096344 | MK096384 | – | MK096429 | MK096304 | – | |
| CFCC 52863 | China | Betula platyphylla | MK096345 | MK096385 | – | MK096430 | MK096305 | – | |
| CFCC 52864 | China | Betula platyphylla | MK096346 | MK096386 | – | MK096431 | MK096306 | – | |
| CFCC 52865 | China | Betula platyphylla | MK096316 | MK096356 | – | MK096401 | MK096276 | – | |
| CFCC 52866 | China | Betula platyphylla | MK096317 | MK096357 | – | MK096402 | MK096277 | – | |
| CFCC 52867 | China | Betula platyphylla | MK096318 | MK096358 | – | MK096403 | MK096278 | – | |
| CFCC 52868 | China | Betula platyphylla | MK096319 | MK096359 | – | MK096404 | MK096279 | – | |
| CFCC 52869 | China | Betula platyphylla | MK096320 | MK096360 | – | MK096405 | MK096280 | – | |
| CFCC 52870 | China | Betula platyphylla | MK096321 | MK096361 | – | MK096406 | MK096281 | – | |
| CFCC 52871 | China | Betula platyphylla | MK096322 | MK096362 | – | MK096407 | MK096282 | – | |
| CFCC 52872 | China | Betula platyphylla | MK096323 | MK096363 | – | MK096408 | MK096283 | – | |
| CFCC 52873 | China | Betula platyphylla | MK096350 | MK096390 | – | MK096435 | MK096310 | – | |
| CFCC 52874 | China | Betula platyphylla | MK096351 | MK096391 | – | MK096436 | MK096311 | – | |
| CFCC 52875 | China | Betula platyphylla | MK096325 | MK096365 | – | MK096410 | MK096285 | – | |
1 Ex-type strains marked by an asterisk; 2 Sequences in bold were generated in the present study