Phosphorus (P) use efficiency in rice is linked to tissue-specific biomass and P allocation patterns

Abstract

Phosphorus (P) is a non-renewable resource which may be depleted within next few decades; hence high P use efficiency is need of time. Plants have evolved an array of adaptive mechanisms to enhance external P acquisition and reprioritize internal utilization under P deficiency. Tissue specific biomass and P allocation patterns may affect the P use efficiency in plants. six rice cultivars were grown in solution culture for 20 days and then were divided into two groups to receive either adequate P or no P that were harvested at 30, 40 and 50 days. Plants were dissected into various tissues/organs. Two rice cultivars viz Super Basmati (P-inefficient) and PS-2 (P-efficient) were grown in soil with no or 50 mg P kg⁻¹ soil till maturity. Rice cultivars PS-2 and Basmati-2000 had higher P uptake, utilization efficiency and internal remobilization than other tested cultivars after P omission. Young leaves and roots were the major sinks while stems and mature leaves were the sources of P during P omission. In conclusion, biomass allocation and P accumulation among various tissues and P remobilization were major factors responsible for P efficiency.

Introduction

Phosphorus (P) is the second-most essential element after nitrogen (N) for its impact on the productivity and health of aquatic and terrestrial ecosystems¹. Being an essential element, P is considered as a major driver for optimum crop productivity on arable land around the globe^2–4. However, suboptimal P availability on arable land forces researchers to determine the mechanisms for improving P acquisition and utilization, and to exploit these responses for the development of P-efficient cultivars^5,6.

The identification of desirable crop plants for adaptation to low-P-input environments may enhance crop productivity and reduce the reliance on costly synthetic P fertilizers⁶. This approach is especially important for developing countries with limited resources. Under the current situation, farmers need P-efficient genotypes that perform better than other genotypes with equivalent P inputs^7–11. Many crop plants have evolved morphological, physiological, biochemical and molecular adaptive systems to cope with P-deficiency stress, such as altered root architecture to explore more soil volume¹², and increased carboxylate exudation containing phosphatases, nucleases and various organic acids^13,14. These mechanisms and strategies are necessary to liberate or solubilize Pi from organic and other insoluble pools¹⁵, enhance Pi uptake capacity¹⁶, recycle internal Pi^13,17, remobilize/retranslocate P from mature to young developing organs^18,19, and reprioritize metabolic P utilization²⁰. Cultivars with enhanced P efficiency could be an alternate strategy for overcoming the dilemma of P deficiency. Phosphorus efficiency can be divided into (i) P acquisition efficiency – the capacity of a cultivar to extract P from soil, and (ii) P utilization efficiency – the capacity of a cultivar to transform the acquired P into biomass/grain yield^21,22.

The process of P remobilization from mature leaves frequently occurs during vegetative growth, when there is insufficient soil P available²³. However, remobilization can also happen during the reproductive period, when new sinks are emerging while further P acquisition by plant roots is reduced²⁴. The remobilization process during reproductive growth is generally associated with foliar senescence to ensure nutrient supply for developing tissues²⁵ and phloem transport is mainly required for the remobilization process, as P is highly mobile in the phloem²⁶. This mechanism of P remobilization is responsible for inter- and intra-specific differences in crop plants for P utilization. These traits are heritable and can be exploited to screen plants for high P-efficiency²⁷.

Rice (Oryza sativa L.) is a major cereal crop known to provide calories to about one third of the population around the globe. According to estimates, global rice harvest removes P from fields of worth around $11 billion each year²⁸. Different rice genotypes with higher P-use efficiency can be utilized in rice yield improvement programs. Enhanced internal P-utilization efficiency is required to complement higher P uptake traits for successful breeding of P efficient rice cultivars²⁹. Screening of existing germplasm and identification of the responsible P-efficiency mechanism is needed to produce more P-efficient cultivars through conventional as well as molecular breeding ventures. The present study was conducted to identify P-efficient rice cultivars and evaluate the responsible mechanisms for P efficiency in two independent experiments.

Results

Experiment 1

Variation in growth response of rice cultivars under P starvation

The rice cultivars had significant (P ≤ 0.05) variations in plant height, root depth, root dry matter (RDM), shoot dry matter (SDM) and root:shoot ratio (RSR) under both adequate P (200 µM Pi) and P deficiency (Table 1). Plant height increased linearly with time at both P levels. Averaged across all harvests, PS-2 and Basmati-2000 had the tallest plants while Super Basmati had the shortest plants at both P levels. Rooting depth of all rice cultivars increased in response to P deficiency relative to adequate P supply. The cultivar PS-2 had the deepest roots at 39.6 cm and 42.8 cm with adequate P and no P, respectively, and KS-282 had the shallowest rooting depth at 27.4 cm with no P. The six rice cultivars varied in RDM with Basmati-2000 producing the maximum (0.53 g plant⁻¹) and Super Basmati producing the minimum (0.20 g plant⁻¹) amounts under P deficiency. The SDM of rice cultivars decreased significantly after exposure to P deficit, but the reduction varied significantly between cultivars. Overall, cultivar PS-2 produced the most SDM (3.31 g plant⁻¹) under adequate P, while Basmati-2000 produced the most (1.36 g plant⁻¹) with no P supplied. The RSR increased in all tested cultivars under P stress except for KS-282 which had no change at either P level. The maximum RSR after P omission (0.45) occurred in KSK-434 (Table 1).

Table 1.

Plant height, root depth, root dry matter, shoot dry matter, and root:shoot ratio of six rice cultivars at 20, 30, 40, and 50 days after transplanting (DAT).

Cultivars	200 µM Pi				0 µM Pi
	20 DAT	30 DAT	40 DAT	50 DAT	30 DAT	40 DAT	50 DAT
Plant height (cm)
Basmati-2000	48.9 ± 0.91 cd	62.3 ± 0.37a	64.8 ± 0.88d	77.5 ± 0.87c	60.2 ± 0.60a	67.4 ± 0.72b	84.0 ± 2.58a
Super Basmati	46.8 ± 0.95d	51.3 ± 0.88c	61.1 ± 0.83e	75.7 ± 0.44 cd	50.3 ± 0.67d	57.4 ± 0.73d	66.4 ± 0.86d
PS-2	58.0 ± 0.70a	61.8 ± 0.88a	83.2 ± 0.43a	90.8 ± 0.62a	59.7 ± 0.44a	73.2 ± 2.62a	76.0 ± 0.76b
KSK-434	50.8 ± 0.68bc	60.5 ± 0.87ab	64.1 ± 0.47d	77.7 ± 0.64c	56.0 ± 0.58b	66.1 ± 0.75b	70.0 ± 0.58c
KS-282	51.6 ± 0.90b	58.7 ± 0.88b	75.6 ± 0.78b	81.0 ± 0.76b	56.7 ± 0.67b	71.7 ± 0.84a	77.0 ± 1.87b
IR-6	51.5 ± 0.77b	59.2 ± 0.73b	67.7 ± 0.94c	74.8 ± 0.79d	53.8 ± 0.15c	61.6 ± 0.72c	63.7 ± 0.73e
LSD0.05	2.6	2.4	2.3	2.2	1.7	3.9	2.3
Root depth (cm)
Basmati-2000	21.7 ± 0.54d	29.0 ± 0.58bc	31.3 ± 1.62b	32.7 ± 0.93bc	28.2 ± 0.60b	31.1 ± 0.96c	42.3 ± 0.44bc
Super Basmati	24.5 ± 0.33c	28.0 ± 2.52bcd	31.5 ± 3.13b	33.2 ± 2.01bc	26.3 ± 0.77bc	28.0 ± 0.66d	41.3 ± 0.44c
PS-2	33.3 ± 0.59a	37.7 ± 0.88a	43.3 ± 0.71a	44.0 ± 2.86a	33.7 ± 0.88a	40.7 ± 0.64a	54.0 ± 0.58a
KSK-434	30.5 ± 0.66b	33.3 ± 3.09ab	34.5 ± 0.29b	35.5 ± 2.80b	33.2 ± 0.73a	36.3 ± 0.79b	44.1 ± 0.75b
KS-282	22.1 ± 0.55d	23.2 ± 0.44 cd	24.1 ± 0.47c	25.3 ± 1.70d	25.3 ± 0.88c	29.3 ± 0.97 cd	27.7 ± 0.88d
IR-6	19.4 ± 0.41e	22.8 ± 2.09d	23.9 ± 1.30c	27.2 ± 2.92 cd	24.7 ± 0.92c	31.1 ± 0.67c	28.2 ± 0.60d
LSD0.05	1.6	4.8	4.9	6.1	2.5	2.4	1.9
Root dry matter (g plant−1)
Basmati-2000	0.30 ± 0.03a	0.40 ± 0.01 cd	0.42 ± 0.05c	0.79 ± 0.01c	0.48 ± 0.03a	0.52 ± 0.09a	0.58 ± 0.01a
Super Basmati	0.16 ± 0.04b	0.48 ± 0.01b	0.47 ± 0.12c	0.65 ± 0.02 cd	0.17 ± 0.03c	0.20 ± 0.09d	0.23 ± 0.07c
PS-2	0.37 ± 0.05a	0.50 ± 0.02b	1.01 ± 0.22a	1.26 ± 0.12ab	0.39 ± 0.04ab	0.45 ± 0.12b	0.49 ± 0.15ab
KSK-434	0.33 ± 0.04a	0.88 ± 0.01a	1.02 ± 0.15a	1.13 ± 0.03b	0.35 ± 0.09b	0.38 ± 0.02c	0.39 ± 0.04b
KS-282	0.20 ± 0.10b	0.42 ± 0.02c	0.71 ± 0.08b	1.42 ± 0.07a	0.22 ± 0.06c	0.23 ± 0.11d	0.20 ± 0.05c
IR-6	0.17 ± 0.02b	0.38 ± 0.01d	0.54 ± 0.11c	0.47 ± 0.04d	0.19 ± 0.04c	0.21 ± 0.05d	0.23 ± 0.08c
LSD0.05	0.13	0.04	0.21	0.19	0.12	0.07	0.18
Shoot dry matter (g plant−1)
Basmati-2000	1.13 ± 0.01a	1.75 ± 0.01bc	1.73 ± 0.18d	4.16 ± 0.45d	1.14 ± 0.02a	1.38 ± 0.14a	0.84 ± 0.10c
Super Basmati	0.51 ± 0.07 cd	1.68 ± 0.07bc	2.36 ± 0.86c	4.36 ± 0.21d	0.55 ± 0.01 cd	0.60 ± 0.09c	0.53 ± 0.05d
PS-2	0.94 ± 0.19b	1.82 ± 0.10b	4.29 ± 0.52a	6.19 ± 0.65a	1.02 ± 0.06a	1.41 ± 0.06a	1.33 ± 0.06a
KSK-434	0.66 ± 0.09c	2.85 ± 0.05a	4.45 ± 0.55a	4.71 ± 0.50 cd	0.72 ± 0.06b	0.92 ± 0.04b	0.91 ± 0.03b
KS-282	0.59 ± 0.11 cd	1.62 ± 0.08c	3.74 ± 0.43b	5.72 ± 0.93b	0.68 ± 0.12bc	0.87 ± 0.05b	0.82 ± 0.04c
IR-6	0.47 ± 0.09d	1.77 ± 0.03bc	2.56 ± 0.54c	4.66 ± 0.21 cd	0.51 ± 0.15d	0.53 ± 0.07c	0.60 ± 0.07bc
LSD0.05	0.19	0.16	0.90	0.69	0.20	0.26	0.26
Root:shoot ratio
Basmati-2000	0.27 ± 0.02d	0.23 ± 0.01 cd	0.14 ± 0.04b	0.19 ± 0.02ab	0.42 ± 0.02ab	0.38 ± 0.03ab	0.69 ± 0.05a
Super Basmati	0.31 ± 0.04 cd	0.29 ± 0.01ab	0.19 ± 0.04ab	0.15 ± 0.01bc	0.31 ± 0.06b	0.40 ± 0.13ab	0.43 ± 0.13b
PS-2	0.45 ± 0.16ab	0.28 ± 0.02ab	0.24 ± 0.01a	0.21 ± 0.04ab	0.38 ± 0.05ab	0.33 ± 0.09ab	0.37 ± 0.11c
KSK-434	0.53 ± 0.09a	0.31 ± 0.01a	0.23 ± 0.01a	0.24 ± 0.02a	0.48 ± 0.11a	0.42 ± 0.03a	0.43 ± 0.06b
KS-282	0.36 ± 0.18 cd	0.27 ± 0.02bc	0.19 ± 0.01ab	0.26 ± 0.05a	0.30 ± 0.10b	0.29 ± 0.05b	0.24 ± 0.05d
IR-6	0.40 ± 0.09bc	0.21 ± 0.01d	0.21 ± 0.02a	0.10 ± 0.01c	0.50 ± 0.09a	0.40 ± 0.11ab	0.38 ± 0.10c
LSD0.05	0.12	0.04	0.06	0.08	0.17	0.12	0.16

Initially, plants were grown for 20 days with adequate P (200 µM Pi). At 20 DAT, three replications of each cultivar were harvested, and the remaining plants were divided into two groups receiving adequate P or no P and harvested at 30, 40, and 50 DAT. Data are shown as means ± standard error (mean ± SE, n = 3). Means sharing identical letter(s) in the same column indicate non-significant differences among cultivars at each harvest (LSD test, P ≤ 0.05).

Genotype-dependent variation in biomass partitioning

The data for dry matter production of various plant tissues, i.e., stem, young leaves (tip and base), and mature leaves (tip and base) growing with or without P is presented in Table 2. Interestingly, stem dry matter declined significantly with P stress in all rice cultivars. With adequate P, rice cultivars PS-2 and Super Basmati produced the maximum (1.49 g plant⁻¹) and minimum (0.97 g plant⁻¹) stem dry matter, respectively, while the corresponding values under P stress were 0.44 g plant⁻¹ in Basmati-2000 and 0.14 g plant⁻¹ in IR-6. Overall, KSK-434 produced the most biomass in the young leaf tip (0.48 g plant⁻¹) and young leaf base (0.53 g plant⁻¹) under adequate P supply, while Basmati-2000 produced the maximum biomass (0.20 and 0.25 g plant⁻¹, respectively) after P omission. The cultivar PS-2 accumulated the most biomass in the mature leaf tip (0.27 g plant⁻¹) and mature leaf base (0.29 g plant⁻¹), and IR-6 produced the least biomass (0.11 g plant⁻¹ and 0.07 g plant⁻¹, respectively) after P omission. However, under adequate P supply, cultivars KSK-434 and Basmati-2000 produced the maximum (0.40 and 0.44 g plant⁻¹) and minimum (0.25 and 0.28 g plant⁻¹) biomass in the mature leaf tip and mature leaf base, respectively. Overall, Basmati-2000 produced the most dry matter in young leaves (tip + base), and PS-2 produced the most in mature leaves (tip + base) under P deficiency.

Table 2.

Dry matter production of various plant tissues (stem, young leaf tip, young leaf base, mature leaf tip, and mature leaf base) of six rice cultivars at 20, 30, 40, and 50 days after transplanting (DAT).

Cultivars	200 µM Pi				0 µM Pi
	20 DAT	30 DAT	40 DAT	50 DAT	30 DAT	40 DAT	50 DAT
Stem (g plant−1)
Basmati-2000	0.25 ± 0.02ab	0.95 ± 0.08ab	0.79 ± 0.13c	2.09 ± 0.26b	0.35 ± 0.04a	0.46 ± 0.03a	0.52 ± 0.11a
Super Basmati	0.15 ± 0.03c	0.86 ± 0.27b	0.99 ± 0.34c	1.86 ± 0.10b	0.17 ± 0.02b	0.17 ± 0.06b	0.15 ± 0.04c
PS-2	0.32 ± 0.08a	0.96 ± 0.10ab	2.01 ± 0.22a	2.66 ± 0.17a	0.33 ± 0.09a	0.37 ± 0.02a	0.33 ± 0.03b
KSK-434	0.20 ± 0.04abc	1.38 ± 0.32a	1.72 ± 0.25ab	1.98 ± 0.20b	0.21 ± 0.04b	0.25 ± 0.03b	0.19 ± 0.07c
KS-282	0.19 ± 0.09bc	0.81 ± 0.22b	1.53 ± 0.18b	2.60 ± 0.23a	0.16 ± 0.03b	0.19 ± 0.05b	0.17 ± 0.03c
IR-6	0.16 ± 0.04bc	0.91 ± 0.20b	1.09 ± 0.19c	2.11 ± 0.09b	0.17 ± 0.02b	0.13 ± 0.04c	0.10 ± 0.03d
LSD0.05	0.11	0.36	0.43	0.48	0.12	0.06	0.08
Young leaf tip (g plant−1)
Basmati-2000	0.22 ± 0.02a	0.17 ± 0.03b	0.25 ± 0.03e	0.49 ± 0.06c	0.17 ± 0.05a	0.19 ± 0.02a	0.23 ± 0.03ab
Super Basmati	0.07 ± 0.02bc	0.17 ± 0.10b	0.40 ± 0.15d	0.69 ± 0.04b	0.07 ± 0.01 cd	0.10 ± 0.01b	0.15 ± 0.04b
PS-2	0.10 ± 0.03b	0.16 ± 0.02b	0.67 ± 0.15ab	0.97 ± 0.25a	0.12 ± 0.02b	0.21 ± 0.03a	0.26 ± 0.05a
KSK-434	0.08 ± 0.01b	0.31 ± 0.08a	0.82 ± 0.06a	0.72 ± 0.12b	0.09 ± 0.03bc	0.16 ± 0.02ab	0.22 ± 0.02ab
KS-282	0.03 ± 0.01c	0.17 ± 0.06b	0.57 ± 0.11c	0.88 ± 0.27ab	0.09 ± 0.01bcd	0.21 ± 0.09a	0.26 ± 0.01a
IR-6	0.04 ± 0.01c	0.18 ± 0.06b	0.38 ± 0.10d	0.69 ± 0.16b	0.05 ± 0.02d	0.11 ± 0.03b	0.15 ± 0.04b
LSD0.05	0.04	0.14	0.13	0.12	0.04	0.09	0.10
Young leaf base (g plant−1)
Basmati-2000	0.14 ± 0.02a	0.23 ± 0.03b	0.27 ± 0.03d	0.56 ± 0.05c	0.21 ± 0.05a	0.25 ± 0.03a	0.28 ± 0.03a
Super Basmati	0.09 ± 0.02ab	0.23 ± 0.10b	0.42 ± 0.15c	0.76 ± 0.10b	0.10 ± 0.02bc	0.14 ± 0.01b	0.11 ± 0.01b
PS-2	0.12 ± 0.03a	0.22 ± 0.02b	0.69 ± 0.15b	1.04 ± 0.25a	0.13 ± 0.02bc	0.18 ± 0.02ab	0.14 ± 0.02b
KSK-434	0.10 ± 0.01ab	0.37 ± 0.06a	0.84 ± 0.06a	0.79 ± 0.12b	0.14 ± 0.02b	0.16 ± 0.03b	0.15 ± 0.02b
KS-282	0.05 ± 0.01b	0.23 ± 0.041b	0.59 ± 0.11b	0.95 ± 0.19ab	0.08 ± 0.03c	0.15 ± 0.04b	0.13 ± 0.01b
IR-6	0.06 ± 0.01b	0.24 ± 0.06b	0.40 ± 0.10 cd	0.76 ± 0.11b	0.09 ± 0.03bc	0.12 ± 0.02b	0.13 ± 0.06b
LSD0.05	0.05	0.12	0.13	0.19	0.06	0.08	0.10
Mature leaf tip (g plant−1)
Basmati-2000	0.14 ± 0.02b	0.17 ± 0.01b	0.19 ± 0.01c	0.49 ± 0.06c	0.18 ± 0.01a	0.21 ± 0.03ab	0.26 ± 0.02b
Super Basmati	0.08 ± 0.01c	0.18 ± 0.07b	0.25 ± 0.12bc	0.51 ± 0.04c	0.08 ± 0.02c	0.09 ± 0.01d	0.11 ± 0.02d
PS-2	0.16 ± 0.05a	0.20 ± 0.03b	0.45 ± 0.02a	0.75 ± 0.12a	0.19 ± 0.02a	0.28 ± 0.08a	0.34 ± 0.06a
KSK-434	0.11 ± 0.02bc	0.36 ± 0.09a	0.52 ± 0.09a	0.59 ± 0.04bc	0.15 ± 0.03ab	0.19 ± 0.03abc	0.21 ± 0.03bc
KS-282	0.13 ± 0.02b	0.17 ± 0.02b	0.50 ± 0.09a	0.63 ± 0.10b	0.17 ± 0.06a	0.16 ± 0.01bc	0.17 ± 0.01 cd
IR-6	0.09 ± 0.02c	0.19 ± 0.06b	0.33 ± 0.08b	0.54 ± 0.07c	0.10 ± 0.06bc	0.11 ± 0.03c	0.13 ± 0.02d
LSD0.05	0.03	0.14	0.12	0.10	0.07	0.12	0.10
Mature leaf base (g plant−1)
Basmati-2000	0.16 ± 0.03ab	0.23 ± 0.01b	0.23 ± 0.02c	0.52 ± 0.04c	0.24 ± 0.01a	0.27 ± 0.04b	0.26 ± 0.02a
Super Basmati	0.12 ± 0.04b	0.24 ± 0.06b	0.29 ± 0.10bc	0.54 ± 0.04c	0.13 ± 0.02c	0.11 ± 0.01 cd	0.05 ± 0.01c
PS-2	0.23 ± 0.08a	0.26 ± 0.03b	0.49 ± 0.04a	0.78 ± 0.12a	0.25 ± 0.01a	0.36 ± 0.03a	0.25 ± 0.02a
KSK-434	0.15 ± 0.02ab	0.42 ± 0.09a	0.56 ± 0.11a	0.62 ± 0.04b	0.13 ± 0.03c	0.16 ± 0.03c	0.14 ± 0.03b
KS-282	0.17 ± 0.02ab	0.23 ± 0.02b	0.54 ± 0.09a	0.66 ± 0.11b	0.18 ± 0.05b	0.15 ± 0.03c	0.10 ± 0.02bc
IR-6	0.13 ± 0.02b	0.25 ± 0.06b	0.37 ± 0.08b	0.57 ± 0.04c	0.09 ± 0.01c	0.07 ± 0.01d	0.05 ± 0.01c
LSD0.05	0.08	0.15	0.14	0.11	0.05	0.08	0.06

Initially, plants were grown for 20 days with adequate P (200 µM Pi). At 20 DAT, three replications of each cultivar were harvested, and the remaining plants were divided into two groups receiving adequate P or no P and harvested at 30, 40, and 50 DAT. Data are shown as means ± standard error (mean ± SE, n = 3). Means sharing identical letter(s) in the same column indicate non-significant differences among cultivars at each harvest (LSD test, P ≤ 0.05).

P acquisition and tissue-specific Pi allocation

The P concentration [P] in different plant tissues, i.e., root, stem, young leaves (tip and base), and mature leaves (tip and base) at 20, 30, 40 and 50 DAT at each P level is given in Table 3. The [P] in plant roots with no P added increased from 20 to 50 DAT. Overall, PS-2 had the highest root [P] with 4.47 and 3.13 mg g⁻¹ and IR-6 had the least [P] with 2.40 and 1.66 mg g⁻¹ under adequate and P stress, respectively. Stem [P] decreased with time in all cultivars under P deficiency. Under P stress, KS-282 had the highest stem [P] (2.73 mg g⁻¹) while Basmati-2000 had the least (1.61 mg g⁻¹). The six rice cultivars varied for [P] in young leaf tips and basal sections at both P levels. After P omission, PS-2 had the highest [P] with 3.18 mg g⁻¹ in young leaf tips while Basmati-2000 had the least [P] with 2.06 mg g⁻¹. The rice cultivars KS-282 and Super Basmati had the highest (3.23 mg g⁻¹) and lowest (2.22 mg g⁻¹) [P] in the young leaf base, respectively, among the tested cultivars under P deficiency. The [P] in mature leaf tips and mature leaf base was higher in IR-6 and KSK-434 (2.36 and 1.73 mg g⁻¹, respectively) after P omission.

Table 3.

Phosphorus concentration [P] of various plant tissues (root, stem, young leaf tip, young leaf base, mature leaf tip, and mature leaf base) of six rice cultivars at 20, 30, 40, and 50 days after transplanting (DAT).

Cultivars	200 µM Pi				0 µM Pi
	20 DAT	30 DAT	40 DAT	50 DAT	30 DAT	40 DAT	50 DAT
Root (mg g−1)
Basmati-2000	2.47 ± 0.21b	4.16 ± 0.30a	4.20 ± 0.28a	5.97 ± 0.19a	2.35 ± 0.54b	2.42 ± 0.04b	2.51 ± 0.16b
Super Basmati	1.66 ± 0.32 cd	3.26 ± 0.15b	3.99 ± 0.36ab	4.28 ± 0.15bc	1.75 ± 0.17bc	1.79 ± 0.15c	1.93 ± 0.25c
PS-2	3.05 ± 0.54a	4.59 ± 0.21a	4.29 ± 0.45a	5.94 ± 0.55a	3.02 ± 0.16a	3.15 ± 0.13a	3.23 ± 0.08a
KSK-434	1.75 ± 0.22c	3.27 ± 0.14b	4.03 ± 0.23ab	4.67 ± 0.27b	1.92 ± 0.32bc	2.46 ± 0.15b	2.62 ± 0.25b
KS-282	1.86 ± 0.29c	2.80 ± 0.27b	3.97 ± 0.36ab	4.16 ± 0.08c	1.89 ± 0.35bc	1.99 ± 0.26c	1.99 ± 0.46c
IR-6	1.34 ± 0.11d	1.56 ± 0.16c	3.57 ± 0.18c	3.14 ± 0.03d	1.47 ± 0.25c	1.69 ± 0.24c	1.83 ± 0.31c
LSD0.05	0.36	0.66	0.70	0.43	0.82	0.43	0.61
Stem (mg g−1)
Basmati-2000	2.91 ± 0.25c	2.56 ± 0.83d	3.87 ± 0.39abc	4.16 ± 0.44c	2.01 ± 0.26b	1.64 ± 0.19b	1.17 ± 0.02b
Super Basmati	2.05 ± 0.13d	2.82 ± 0.31d	3.55 ± 0.14c	3.23 ± 0.29d	1.74 ± 0.13b	1.78 ± 0.26ab	1.47 ± 0.23b
PS-2	4.03 ± 0.14ab	5.82 ± 0.56ab	3.81 ± 0.53bc	5.01 ± 0.09ab	3.83 ± 0.13a	1.48 ± 0.09b	1.32 ± 0.26b
KSK-434	4.56 ± 0.11a	6.52 ± 0.50a	3.94 ± 0.12abc	5.39 ± 0.71a	3.97 ± 0.23a	1.59 ± 0.13b	1.51 ± 0.40b
KS-282	3.90 ± 0.27b	4.47 ± 0.78bc	4.43 ± 0.15a	4.55 ± 0.59bc	3.78 ± 0.24a	2.25 ± 0.36a	2.17 ± 0.39a
IR-6	2.01 ± 0.15d	3.63 ± 0.34 cd	4.21 ± 0.30ab	4.49 ± 0.18bc	1.93 ± 0.33b	1.81 ± 0.57ab	1.55 ± 0.26b
LSD0.05	0.57	1.87	0.64	0.86	0.88	0.56	0.62
Young leaf tip (mg g−1)
Basmati-2000	2.53 ± 0.05ab	3.49 ± 0.41bc	3.49 ± 0.10ab	3.44 ± 0.51c	1.99 ± 0.13b	2.04 ± 0.19c	2.16 ± 0.04c
Super Basmati	1.75 ± 0.15c	2.98 ± 0.12c	3.11 ± 0.21bc	2.52 ± 0.20d	2.56 ± 0.51ab	2.57 ± 0.12b	2.63 ± 0.34bc
PS-2	2.69 ± 0.12a	4.72 ± 0.37a	3.75 ± 0.44a	4.18 ± 0.51b	2.77 ± 0.21a	3.33 ± 0.53a	3.45 ± 0.28a
KSK-434	2.58 ± 0.24a	3.74 ± 0.26bc	3.75 ± 0.49a	5.38 ± 0.21a	2.57 ± 0.26ab	2.70 ± 0.26b	3.15 ± 0.37a
KS-282	2.44 ± 0.16ab	4.34 ± 0.32ab	4.01 ± 0.30a	4.57 ± 0.54b	2.81 ± 0.30a	2.98 ± 0.60ab	3.01 ± 0.09ab
IR-6	1.91 ± 0.10bc	3.68 ± 0.47bc	2.75 ± 0.24c	2.93 ± 0.46 cd	2.02 ± 0.24b	2.19 ± 0.41c	2.23 ± 0.57c
LSD0.05	0.67	0.96	0.61	0.52	0.71	0.32	0.53
Young leaf base (mg g−1)
Basmati-2000	3.17 ± 0.12ab	3.83 ± 0.19c	4.24 ± 0.69a	4.03 ± 0.48a	3.43 ± 0.15a	2.95 ± 0.13b	2.96 ± 0.06a
Super Basmati	3.24 ± 0.21ab	5.19 ± 0.35b	3.98 ± 0.47ab	3.59 ± 0.15ab	1.98 ± 0.45c	2.40 ± 0.29c	2.28 ± 0.07b
PS-2	3.31 ± 0.20ab	3.66 ± 0.28c	3.45 ± 0.12ab	3.46 ± 0.19ab	2.84 ± 0.40b	3.04 ± 0.07ab	2.94 ± 0.12a
KSK-434	3.66 ± 0.05a	6.14 ± 0.48a	3.41 ± 0.51bc	3.05 ± 0.25b	3.19 ± 0.59ab	3.17 ± 0.11ab	2.96 ± 0.41a
KS-282	3.39 ± 0.17ab	3.99 ± 0.42c	2.58 ± 0.33c	2.37 ± 0.27c	3.59 ± 0.26a	3.43 ± 0.43a	2.67 ± 0.39ab
IR-6	3.13 ± 0.17b	5.52 ± 0.18b	3.43 ± 0.10ab	3.74 ± 0.63a	3.24 ± 0.12ab	3.20 ± 0.25ab	2.83 ± 0.55ab
LSD0.05	0.50	0.62	0.83	0.65	0.57	0.47	0.63
Mature leaf tip (mg g−1)
Basmati-2000	3.42 ± 0.26a	3.10 ± 0.26c	2.32 ± 0.27c	2.86 ± 0.28bc	2.70 ± 0.27ab	2.27 ± 0.28a	1.38 ± 0.07b
Super Basmati	1.99 ± 0.19d	5.65 ± 0.82a	3.15 ± 0.15a	3.69 ± 0.35a	2.09 ± 0.12b	1.56 ± 0.13bc	1.60 ± 0.10ab
PS-2	2.50 ± 0.40bcd	3.38 ± 0.70c	2.65 ± 0.03bc	2.77 ± 0.26bc	2.80 ± 0.35ab	1.49 ± 0.26bc	1.52 ± 0.24b
KSK-434	3.08 ± 0.09ab	4.45 ± 0.83b	3.13 ± 0.13a	3.51 ± 0.24ab	3.23 ± 0.33a	1.99 ± 0.09ab	1.60 ± 0.20ab
KS-282	2.21 ± 0.34 cd	3.54 ± 0.10c	2.77 ± 0.64ab	3.04 ± 0.13abc	2.29 ± 0.38b	1.38 ± 0.19c	1.44 ± 0.08b
IR-6	2.91 ± 0.10abc	4.13 ± 0.25b	2.64 ± 0.28bc	2.74 ± 0.30c	3.24 ± 0.45a	1.75 ± 0.35abc	2.08 ± 0.51a
LSD0.05	0.79	0.87	0.44	0.72	0.81	0.59	0.55
Mature leaf base (mg g−1)
Basmati-2000	2.16 ± 0.18a	3.34 ± 0.11 cd	2.52 ± 0.64c	2.27 ± 0.09d	2.40 ± 0.48a	1.38 ± 0.18ab	1.07 ± 0.03b
Super Basmati	1.53 ± 0.17bc	4.98 ± 0.46a	3.77 ± 0.14b	4.30 ± 0.42a	1.77 ± 0.16b	1.53 ± 0.15a	1.36 ± 0.06a
PS-2	1.63 ± 0.33bc	3.76 ± 0.07bc	3.52 ± 0.13b	3.68 ± 0.43b	1.68 ± 0.20b	1.40 ± 0.14a	1.19 ± 0.13ab
KSK-434	2.20 ± 0.27a	4.88 ± 0.78a	4.31 ± 0.52a	4.54 ± 0.32a	2.30 ± 0.11a	1.48 ± 0.11a	1.39 ± 0.12a
KS-282	1.97 ± 0.18ab	2.87 ± 0.33d	2.81 ± 0.27c	3.07 ± 0.20c	2.24 ± 0.14a	1.44 ± 0.24a	1.18 ± 0.04ab
IR-6	1.73 ± 0.23bc	3.97 ± 0.10b	2.76 ± 0.36c	2.82 ± 0.07c	1.36 ± 0.15b	1.22 ± 0.18b	1.56 ± 0.24a
LSD0.05	0.42	0.62	0.41	0.59	0.47	0.15	0.29

Initially, plants were grown for 20 days with adequate P (200 µM Pi). At 20 DAT, three replications of each cultivar were harvested, and the remaining plants were divided into two groups receiving adequate P or no P and harvested at 30, 40, and 50 DAT. Data are shown as means ± standard error (mean ± SE, n = 3). Means sharing identical letter(s) in the same column indicate non-significant differences among cultivars at each harvest (LSD test, P ≤ 0.05).

The cultivars varied significantly (P ≤ 0.05) in P uptake in different plant parts when grown under adequate P or P stress (Table 4). The highest root P uptake under adequate P or P stress was in PS-2 with 3.77 and 1.38 mg plant⁻¹, respectively. Likewise, PS-2 had higher stem P uptake at adequate P supply and after P stress (6.96 mg plant⁻¹ and 0.73 mg plant⁻¹, respectively). The maximum P uptake (0.63 mg plant⁻¹) by young leaf tips occurred in PS-2 while the minimum (0.21 mg plant⁻¹) occurred in IR-6 under P stress. Basmati-2000 and Super Basmati had the highest (0.76 mg plant⁻¹) and lowest (0.26 mg plant⁻¹) P uptake by the young leaf base after P omission. Mature leaf tips accumulated higher P contents than the mature leaf base after P omission. PS-2 had the highest P uptake by the mature tip and basal sections (0.49 and 0.41 mg plant⁻¹, respectively) during P stress. When comparing P uptake between young and mature leaves, young leaves had higher P uptake at adequate P and under P stress. Averaged across all harvests, PS-2 had the highest total P uptake (4.10 mg plant⁻¹) while IR-6 had the lowest (1.35 mg plant⁻¹) under P stress.

Table 4.

Phosphorus uptake (mg plant⁻¹) by various plant tissues (root, stem, young leaf tip, young leaf base, mature leaf tip, mature leaf base, and whole plant) in six rice cultivars at 20, 30, 40, and 50 days after transplanting (DAT).

Cultivars	200 µM Pi				0 µM Pi
	20 DAT	30 DAT	40 DAT	50 DAT	30 DAT	40 DAT	50 DAT
Root (mg plant−1)
Basmati-2000	0.76 ± 0.12b	1.68 ± 0.16c	0.96 ± 0.14d	4.72 ± 0.15d	1.11 ± 0.25a	1.27 ± 0.23a	1.45 ± 0.11a
Super Basmati	0.29 ± 0.10de	1.57 ± 0.06c	1.97 ± 0.21c	2.79 ± 0.16e	0.30 ± 0.06c	0.31 ± 0.11c	0.42 ± 0.09c
PS-2	1.17 ± 0.34a	2.29 ± 0.05b	4.21 ± 0.44a	7.41 ± 0.64a	1.16 ± 0.10a	1.40 ± 0.33a	1.58 ± 0.48a
KSK-434	0.60 ± 0.15c	2.89 ± 0.14a	4.05 ± 0.13a	5.25 ± 0.19c	0.61 ± 0.06b	0.95 ± 0.08b	1.01 ± 0.02b
KS-282	0.41 ± 0.26d	1.19 ± 0.15d	2.77 ± 0.31b	5.92 ± 0.18b	0.34 ± 0.11c	0.37 ± 0.12c	0.37 ± 0.09c
IR-6	0.24 ± 0.04e	0.59 ± 0.05e	1.89 ± 0.11c	1.48 ± 0.12 f	0.29 ± 0.10c	0.31 ± 0.02c	0.32 ± 0.04c
LSD0.05	0.15	0.22	0.52	0.53	0.16	0.13	0.36
Stem (mg plant−1)
Basmati-2000	0.74 ± 0.11b	2.28 ± 0.73d	3.02 ± 0.52c	8.52 ± 0.69d	0.75 ± 0.28b	0.76 ± 0.13a	0.60 ± 0.12a
Super Basmati	0.32 ± 0.07c	2.60 ± 0.96d	3.60 ± 0.29bc	6.01 ± 0.64e	0.29 ± 0.05d	0.27 ± 0.08d	0.20 ± 0.04c
PS-2	1.33 ± 0.28a	5.66 ± 1.02c	7.54 ± 0.95a	13.32 ± 0.64a	1.24 ± 0.14a	0.54 ± 0.01b	0.42 ± 0.05b
KSK-434	0.92 ± 0.18b	9.15 ± 2.70a	6.75 ± 0.91a	10.37 ± 0.18c	0.83 ± 0.05b	0.39 ± 0.03c	0.24 ± 0.05c
KS-282	0.79 ± 0.14b	3.31 ± 0.37bc	6.82 ± 0.94a	11.77 ± 1.57b	0.57 ± 0.22c	0.39 ± 0.05c	0.37 ± 0.10b
IR-6	0.33 ± 0.10c	3.37 ± 0.97b	4.65 ± 0.85b	9.46 ± 0.34 cd	0.24 ± 0.03d	0.20 ± 0.03d	0.14 ± 0.03c
LSD0.05	0.27	0.73	1.24	1.38	0.15	0.07	0.13
Young leaf tip (mg plant−1)
Basmati-2000	0.50 ± 0.06a	0.57 ± 0.02c	3.74 ± 0.09bc	1.74 ± 0.43b	0.33 ± 0.10a	0.39 ± 0.04c	0.55 ± 0.06c
Super Basmati	0.13 ± 0.05 cd	0.52 ± 0.33c	3.51 ± 0.06c	1.75 ± 0.23b	0.19 ± 0.06b	0.25 ± 0.02d	0.35 ± 0.06d
PS-2	0.27 ± 0.09b	0.77 ± 0.07b	4.42 ± 0.45ab	3.83 ± 0.57a	0.34 ± 0.07a	0.69 ± 0.11a	0.87 ± 0.12a
KSK-434	0.21 ± 0.03bc	1.18 ± 0.32a	4.57 ± 0.44a	3.92 ± 0.75a	0.23 ± 0.08b	0.44 ± 0.03bc	0.68 ± 0.09b
KS-282	0.09 ± 0.04d	0.71 ± 0.23b	4.58 ± 0.40a	4.30 ± 1.82a	0.25 ± 0.01b	0.53 ± 0.16b	0.77 ± 0.06ab
IR-6	0.07 ± 0.03d	0.65 ± 0.17bc	3.13 ± 0.34c	1.89 ± 0.32b	0.11 ± 0.05c	0.21 ± 0.03d	0.30 ± 0.02d
LSD0.05	0.10	0.14	0.73	0.66	0.06	0.11	0.13
Young leaf base (mg plant−1)
Basmati-2000	0.43 ± 0.06a	0.89 ± 0.14 cd	4.51 ± 0.70a	2.22 ± 0.21b	0.72 ± 0.20a	0.74 ± 0.14a	0.83 ± 0.11a
Super Basmati	0.28 ± 0.05bc	1.14 ± 0.47bc	4.40 ± 0.61a	2.72 ± 0.16ab	0.21 ± 0.08d	0.33 ± 0.02d	0.25 ± 0.01c
PS-2	0.42 ± 0.10a	0.83 ± 0.12d	4.13 ± 0.13ab	3.50 ± 0.65a	0.36 ± 0.06bc	0.55 ± 0.05b	0.43 ± 0.07b
KSK-434	0.37 ± 0.04ab	2.30 ± 0.56a	4.24 ± 0.57a	2.36 ± 0.22ab	0.42 ± 0.06b	0.51 ± 0.07b	0.44 ± 0.06b
KS-282	0.19 ± 0.05c	0.95 ± 0.28 cd	3.17 ± 0.36b	2.20 ± 0.57b	0.28 ± 0.07 cd	0.47 ± 0.07bc	0.35 ± 0.06bc
IR-6	0.18 ± 0.04c	1.34 ± 0.34b	3.82 ± 0.17ab	2.63 ± 0.21ab	0.31 ± 0.11 cd	0.39 ± 0.09 cd	0.30 ± 0.10bc
LSD0.05	0.12	0.26	1.04	1.19	0.10	0.11	0.16
Mature leaf tip (mg plant−1)
Basmati-2000	0.93 ± 0.15a	0.51 ± 0.03e	0.44 ± 0.08d	1.39 ± 0.12b	0.48 ± 0.05a	0.47 ± 0.06a	0.35 ± 0.02b
Super Basmati	0.16 ± 0.03d	1.02 ± 0.41b	0.78 ± 0.19c	1.86 ± 0.12ab	0.18 ± 0.04c	0.14 ± 0.01d	0.17 ± 0.03d
PS-2	0.44 ± 0.11b	0.69 ± 0.19 cd	1.19 ± 0.08b	2.09 ± 0.45a	0.55 ± 0.12a	0.41 ± 0.11b	0.51 ± 0.07a
KSK-434	0.36 ± 0.07bc	1.47 ± 0.13a	1.60 ± 0.26a	2.09 ± 0.25a	0.48 ± 0.04a	0.37 ± 0.05b	0.33 ± 0.03bc
KS-282	0.31 ± 0.08bcd	0.61 ± 0.09de	1.32 ± 0.22b	1.95 ± 0.40ab	0.35 ± 0.09b	0.23 ± 0.04c	0.24 ± 0.02 cd
IR-6	0.26 ± 0.05 cd	0.76 ± 0.19c	0.89 ± 0.28c	1.48 ± 0.29b	0.27 ± 0.13bc	0.16 ± 0.03d	0.22 ± 0.08 cd
LSD0.05	0.16	0.13	0.28	0.41	0.13	0.05	0.11
Mature leaf base (mg plant−1)
Basmati-2000	0.55 ± 0.04a	0.75 ± 0.11 cd	0.58 ± 0.17d	1.19 ± 0.13d	0.58 ± 0.14a	0.36 ± 0.04b	0.28 ± 0.02a
Super Basmati	0.20 ± 0.08d	1.24 ± 0.43b	1.10 ± 0.45c	2.29 ± 0.10abc	0.23 ± 0.02c	0.15 ± 0.04de	0.07 ± 0.01c
PS-2	0.44 ± 0.14b	0.99 ± 0.11bc	1.71 ± 0.12b	2.76 ± 0.21ab	0.42 ± 0.04b	0.50 ± 0.06a	0.30 ± 0.04a
KSK-434	0.36 ± 0.08bc	2.20 ± 0.69a	2.30 ± 0.13a	2.86 ± 0.40a	0.29 ± 0.07c	0.23 ± 0.02c	0.18 ± 0.03b
KS-282	0.35 ± 0.05bc	0.66 ± 0.09d	1.57 ± 0.40b	2.06 ± 0.39bc	0.39 ± 0.08b	0.21 ± 0.01 cd	0.12 ± 0.02bc
IR-6	0.24 ± 0.05 cd	1.00 ± 0.24bc	1.01 ± 0.24c	1.59 ± 0.08 cd	0.13 ± 0.02d	0.09 ± 0.02e	0.08 ± 0.02c
LSD0.05	0.14	0.29	0.39	0.79	0.08	0.07	0.08
Whole plant (mg plant−1)
Basmati-2000	3.96 ± 0.14a	6.69 ± 0.81c	13.25 ± 0.51d	19.77 ± 1.09c	3.97 ± 0.24a	3.99 ± 0.64a	4.01 ± 0.40a
Super Basmati	1.38 ± 0.28d	8.08 ± 0.72c	15.36 ± 2.47c	17.43 ± 0.91c	1.40 ± 0.09d	1.44 ± 0.08d	1.46 ± 0.09c
PS-2	4.07 ± 0.42a	11.23 ± 0.61b	23.20 ± 0.64a	32.92 ± 1.18a	4.08 ± 0.17a	4.10 ± 0.23a	4.11 ± 0.56a
KSK-434	2.83 ± 0.32b	19.19 ± 0.97a	23.52 ± 1.96a	26.84 ± 1.46b	2.86 ± 0.11b	2.87 ± 0.14b	2.88 ± 0.09b
KS-282	2.15 ± 0.28c	7.44 ± 0.44c	20.23 ± 1.90b	28.19 ± 3.26b	2.18 ± 0.40c	2.19 ± 0.12c	2.21 ± 0.25bc
IR-6	1.32 ± 0.21d	7.71 ± 0.24c	15.40 ± 2.38c	18.54 ± 1.06c	1.34 ± 0.16d	1.35 ± 0.07d	1.36 ± 0.13c
LSD0.05	0.46	1.63	1.89	3.23	0.48	0.41	0.95

Initially, plants were grown for 20 days with adequate P (200 µM Pi). At 20 DAT, three replications of each cultivar were harvested, and the remaining plants were divided into two groups receiving adequate P or no P and harvested at 30, 40, and 50 DAT. Data are shown as means ± standard error (mean ± SE, n = 3). Means sharing identical letter(s) in the same column indicate non-significant differences among cultivars at each harvest (LSD test, P ≤ 0.05).

Internal remobilization and utilization efficiency of acquired P by rice cultivars

The internal P remobilization (IPR) was calculated among various plant tissues at 50 days of transplanting after feeding plants for 20 days with adequate P and subsequent P omission for next 30 days (Fig. 1). At this time, P had accumulated in the roots and young leaves after remobilization from the stem and mature leaves. Cultivars KSK-434 and PS-2 remobilized the most stem P reserves (74 and 68%, respectively) after P omission. The lowest IPR from stems occurred in Basmati-2000, but its roots accumulated the most P (92%) during P omission. Among young leaves, the apical section (tip) accumulated comparatively more P reserves than the basal section in all cultivars except Super Basmati where P contents declined upto 11% for the young leaf base. Nevertheless, basal sections of mature leaves remobilized more P than the mature leaf apical (tip) section in all cultivars except PS-2 and Super Basmati where P accumulation (16 and 7%, respectively) occurred in the mature leaf tip. Phosphorus utilization efficiency (PUTE) varied from 12.1% (Super Basmati) to 21.7% (PS-2) with a mean value of 16.9% (Fig. 2).

Figure 1.

Internal phosphorus remobilization among various plant tissues of six rice cultivars at 50 days of transplanting after feeding plants for 20 days with adequate P level (200 µM Pi) and subsequent P omission for next 30 days. Positive signs indicate P accumulation while negative signs illustrate P remobilization in specific plant tissues/organs in response to P omission.

Figure 2.

Phosphorus utilization efficiency (PUTE) of six rice cultivars calculated at two P levels under hydroponic conditions (Experiment 1). Values are means of three replicates (n = 3) and presented with standard error. Bars not sharing identical letter(s) are significantly different from each other (LSD test, P ≤ 0.05). LSD value for PUTE at P ≤ 0.05 is 5.53.

Experiment 2

Two rice cultivars contrasting in P utilization efficiency - Super Basmati (P-inefficient) and PS-2 (P-efficient) - were selected from Experiment 1 and grown at two P levels (0 and 50 mg P kg⁻¹) in soil-filled pots to maturity to investigate their response for paddy production, P acquisition and utilization efficiencies.

Yield and related attributes

Phosphorus deficiency significantly (P ≤ 0.05) reduced rice yield and related attributes, i.e., plant height, tiller number and panicle length, in both rice cultivars (Table 5, Fig. 3). However, the magnitude of reduction was higher in cultivar Super Basmati than cultivar PS-2. Plant height of Super Basmati and PS-2 varied from 42.3–48.0 cm and 63.0–73.0 cm under deficient and adequate P supply, respectively. Super Basmati had more tillers under P deficiency than PS-2 (13.7 vs. 9.3). The panicle length of Super Basmati and PS-2 increased from 8.7 to 14.3 cm and 13.7 to 18.0 cm in response to deficient and adequate P, respectively. PS-2 produced the highest paddy yield (18.8 vs. 29.0 g pot⁻¹) as compared to Super Basmati (8.7 vs. 15.8 g pot⁻¹) in unfertilized and P-fertilized plants, respectively. Straw yield in PS-2 and Super Basmati declined in unfertilized plants (38.2 and 20.5 g pot⁻¹) and increased in response to P fertilization (44.4 and 30.9 g pot⁻¹), respectively. The biological yield (straw + paddy) of PS-2 and Super Basmati was 73.4 and 46.7 g pot⁻¹ in P-fertilized plants and 57.0 and 29.2 g pot⁻¹ in P-deficient plants, respectively.

Table 5.

Analysis of variance for the combined effect of phosphorus level and rice cultivar on various growth parameters of soil-grown rice plants.

Source of variation	DF	PH	NTP	PL	PY	SY	BY	PPU	SPU	TPU
Phosphorus levels (P)	1	184.08***	65.33***	75.00***	223.60***	205.84***	858.52***	5160.5***	4564.6***	19431.9***
Cultivars (C)	1	1564.08***	48.00**	56.33***	406.00***	731.64***	2227.69***	11561.2***	4084.4***	29390.1***
P × C	1	14.08*	0.33 ns	1.33 ns	7.68*	13.44*	0.80 ns	214.6**	26.1*	91.1*
Error	8	1.58	2.00	0.87	1.11	3.43	4.46	13.4	6.4	24.8
Total	11

DF = degrees of freedom; PH = plant height; NTP = tiller number per pot; PL = panicle length; PY = paddy yield; SY = straw yield; BY = biological yield; PPU = paddy P uptake; SPU = straw P uptake; TPU = total (paddy + straw) P uptake.

***significant at P ≤ 0.001; **significant at P ≤ 0.01; *significant at P ≤ 0.05; ns = non-significant at P ≥ 0.05.

Figure 3.

Plant height (a), tiller number (b), panicle length (c), paddy yield (d), straw yield (e), biological yield (f), paddy P uptake (g), straw P uptake (h), and total P uptake (i) of two rice cultivars selected from Experiment 1. Values are means of three replicates (n = 3) and presented with standard error. Bars not sharing identical letter(s) are significantly different from each other at two P levels (LSD test, P ≤ 0.05).

Phosphorus uptake and efficiency relations of rice cultivars

Phosphorus uptake in paddy, straw, and total (paddy + straw) by rice cultivars grown under soil conditions with deficient and adequate P supply is depicted in Fig. 3. The rice cultivars and P levels had significant (P ≤ 0.05) main and interactive effects on P uptake by paddy and straw (Table 5). Average P uptake by both straw and paddy was about two-fold higher in plants with adequate P supply than deficient levels. The paddy P uptake in PS-2 and Super Basmati was 130.81 and 60.27 mg pot⁻¹ under adequate P and 80.87 and 27.25 mg pot⁻¹ under P deficiency, respectively. Under adequate P, straw P uptake in PS-2 and Super Basmati was 95.50 and 61.54 mg pot⁻¹ and 59.44 and 19.59 mg pot⁻¹ under P deficiency, respectively. The values for total P uptake ranged from 140.31 to 226.30 mg pot⁻¹ for PS-2 and 46.84 to 121.81 mg pot⁻¹ for Super Basmati under deficient and adequate P, respectively.

Both rice cultivars responded differently for P-efficiency relations – P acquisition efficiency (PACE), P utilization efficiency (PUTE) and P stress factor (PSF) – at the two soil P levels (Fig. 4). The rice cultivar PS-2 had higher values than Super Basmati for PACE (14.3 vs. 9.4%) and PUTE (64.7 vs. 55.8%). The PSF describes the percent reduction in paddy production in response to P deficiency in the rooting medium. It distinguishes between P-responsive and non-responsive genotypes and elucidates the capacity of a genotype to produce biomass with P addition. Super Basmati had a larger PSF (44.2%) than PS-2 (35.3%).

Figure 4.

Phosphorus acquisition efficiency (PACE), phosphorus utilization efficiency (PUTE), and phosphorus stress factor (PSF) of two rice cultivars grown under soil conditions (Experiment 2). Values are means of three replicates (n = 3) and presented with standard error. For each parameter, bars not sharing identical letter(s) are significantly different from each other (LSD test, P ≤ 0.05). LSD values for PACE, PUTE, and PSF at P ≤ 0.05 are 2.88, 7.22, and 8.73, respectively.

Discussion

Limited P availability to plants on arable land is a major nutritional constraint hindering plant growth and crop yields. Significant variation for P absorption and utilization among crop species and even genotypes within the same species is well documented^4,5,10,30,31. The selection/identification of cultivars that can absorb and use P efficiently is a promising strategy to cope with environments deficient in bio-available P¹². Phosphorus is a highly mobile element within plants that is readily remobilized/re-translocated from metabolically inactive to active sites to sustain plant growth under P deficiency³². In this study, we investigated the hypothesis that variation in P acquisition and remobilization is due to adaptive mechanisms that cause differential P utilization in rice cultivars.

In Experiment 1, root and shoot dry matter (DM) production under P deficiency varied between rice cultivars. Dry matter production under low-P supply is an excellent parameter for screening germplasm for higher yields and P efficiency^8,33. Two efficient cultivars, PS-2 and Basmati-2000, produced more DM than Super Basmati and IR-6 under P starvation, which can be attributed to an increased rate of P remobilization to growing sites, i.e., young leaves and roots. Remobilization of stored P in the stem and older leaves to metabolically active sites may supplement the restricted P supply under P deficiency^13,34,35. This was confirmed at all harvests by the differential P redistribution and accumulation in various plant tissues during P omission (Fig. 1, Table 4). Variations in shoot biomass under varying levels of P among different rice cultivars have been reported³⁶. Roots are critical for plant growth and are directly exposed to the soil environment. As P mobility in soil is limited, higher plant root growth and changes in root morphology is helpful for more P uptake. Rice cultivar PS-2 had the deepest roots and the most root dry matter when exposed to P deficiency (Table 1). Under P starvation, root length and root hair density significantly increased³⁷ for better P absorption. The findings of Bates and Lynch³⁸ suggested that increased root growth is associated with improved plant performance under low P by exploring a larger volume of soil. The root:shoot ratio (RSR) is a further indication of the partitioned plant growth into roots and shoots to differential P supply. The RSR increased in all tested cultivars under P stress while KSK-434 had the maximum RSR. Generally, under low-P supply, plant growth is suppressed. However, the relative effect is most dominant in the case of shoot growth. Consequently, RSR increases significantly in low-P environments³⁹ and is an excellent index for partitioning photosynthesized carbon between above – and below – ground plant parts. Root density and RSR generally increased under P deficiency, thus favoring P acquisition by plants^13,40. Plants often allocate a greater portion of their biomass to the roots in response to P deficient environment. Such acclamatory response is a consequence of metabolic changes in shoot and an adjustment of carbohydrate transport to the root. P deficiency alter root-to-shoot biomass ratio by accumulation of carbohydrates in leaves and roots⁴¹. These findings were confirmed in the current study.

Increased remobilization of absorbed P among various tissues within plants under P deficiency might be a mechanism for better P efficiency in cultivars differing in P acquisition and utilization^32,42. In Experiment 1, plants were grown for 20 days with adequate P (200 µM Pi), after which P was omitted from the solution for the next 30 days. As plants were unable to uptake more P from the medium, they had to remobilize previously acquired P from various plant tissues. Molecular mechanisms involved in mobilizing P from different plant tissues/organs are well reported^15,43–45. Organic pools of P in plant tissues mainly exist as nucleic acids, phospholipids, phosphorylated proteins and P-ester metabolites¹⁵. Under P deficiency, phospholipids have greater potential to serve as an alternate source of P which remobilizes towards young growing plant tissues⁴⁶. This phenomenon of replacing phospholipids in cell membranes with sulfolipids and galactolipids is known as membrane-lipid remodeling⁴³. Lipid remodeling occurs both in roots and shoots of P-efficient rice genotypes under low-P conditions resulting in enhanced PUE⁴⁴. Ribonucleases and intracellular phosphatases had crucial role in determining the amount of P to be remobilized from older to young green leaves and developing grains⁴⁵.

During P omission, P accumulated in the roots and young leaves after remobilization from the stem and mature leaves (Fig. 1, Table 4). Cultivars KSK-434 and PS-2 remobilized the most stem P reserves (74 and 68%, respectively) after P omission. Within young leaves, the apical section (tip) accumulated comparatively more P than the basal section (Table 4). Based on differential P contents in various plant tissues during P omission; it could be assumed that P remobilization from the metabolically inactive pool to the active pool was initially more intensive in mature leaves, stem, roots, and young leaves. With persistent P stress, the stems recorded the maximum reduction in P contents. An increase or even maintenance of root P content during P stress may be related to greater allocation of assimilates to roots⁴⁷. The higher P contents in roots and young leaves of rice under adequate P illustrates that young tissues have better storage capacity than older tissues¹⁷. Moreover, enhanced metabolic activities of young tissues make them stronger sinks for the already absorbed P. After removing P from the nutrient solution, plant growth may occur due to remobilization and utilization of absorbed P⁴⁸. According to Martinez et al.⁴⁹, young leaves and roots were the major sinks of stored P in soybean cultivars. Under moderate P deficiency, the transfer of P increased in the shoots of potato plants, while severely stressed plants retained P in the roots⁵⁰. Although, more P acquisition form P-deficient medium is necessary for tolerance of a genotype to low P, however efficient remobilization of acquired P to different growing tissues can make a genotype well adapted to low-P environment⁵¹. Efficient internal utilization of P is generally attributed because of high photosynthetic activity per unit of P and more efficient P remobilization from older to young leaves⁵². Moreover, higher remobilization of P from older leaves to young green leaves in P-efficient rice genotypes is important to produce more photosynthates which ultimately contribute for biomass/yield production⁵³.

In Experiment 2, two rice cultivars selected from the hydroponic study – Super Basmati (P-inefficient) and PS-2 (P-efficient) – were grown in soil to maturity to investigate their response for paddy production, P acquisition, and utilization efficiencies. It was clear from the results that the high-yielding cultivar PS-2 had more paddy P uptake and the low-yielding cultivar Super Basmati had the lowest paddy P uptake under both P levels. Fageria⁵⁴ also reported increased P uptake in grains of high-yielding rice genotypes at low P. According to Baligar et al.⁵⁵, more P uptake in plants grown at high P compared with those at low P is due to an increased root fine hair density. The rice cultivars varied in P-efficiency relations (PACE, PUTE, and PSF) under P stress. The high-yielding PS-2 had greater PACE and PUTE than the low-yielding Super Basmati. Phosphorus utilization and uptake efficiencies are important for selecting genotypes under P-deficiency stress. The P-use efficiency of cultivars is associated with the amount of dry matter produced per unit of absorbed P⁵⁶. The variation between cultivars for PUE might be due to differential P absorption characteristics or distribution patterns within plants. Both cultivars varied in their relative reduction in paddy yield due to P deficiency (PSF). The PSF value distinguishes between P-responsive and non-responsive cultivars and the comparative ability of a cultivar to produce biomass with P addition^4,5,13. Cultivar PS-2 had a lower PSF value than Super Basmati under P stress (35% vs. 44%). Under P deficiency, PS-2 produced more paddy yield than Super Basmati and responded well to P application. Super Basmati was less desirable due to poor performance under varying P supply. PS-2 also had higher P uptake than Super Basmati, indicating an important contributing trait to its high P-efficiency. The lower P-efficiency of Super Basmati was further supported by its higher PSF value than PS-2. Fageria et al.³⁰ reported variation in the abilities of lowland rice cultivars to absorb P, and its remobilization and efficient utilization overtime. Plant traits associated with efficient P acquisition and internal utilization are heritable and can be exploited more extensively to develop P-efficient cultivars²⁷.

Conclusions

Significant genetic variation exists among the tested rice cultivars for P efficiency. Rice cultivars with higher P uptake, acquisition efficiency, utilization efficiency and lower PSF values were efficient (low-P tolerant) and thus more desirable for adaptation to soils low in available P. The cultivars with high biomass/paddy yield at both P levels (PS-2 and Basmati-2000) have better internal remobilization of absorbed P and photosynthate translocation to young/active plant tissues. Hence, P partitioning and biomass allocation to roots should be considered when screening germplasm in P-efficiency programs.

Materials and Methods

Experiment 1

Plant material, growth conditions, and management

Seeds of six rice cultivars (Basmati-2000, Super Basmati, PS-2, KSK-434, KS-282 and IR-6) were kindly provided by the Rice Research Institute, Kala Shah Kaku, Pakistan. Seeds were surface sterilized with 3% sodium hypochlorite solution and sown in polyethylene foil-lined metal trays containing two inches of washed riverbed sand. Distilled water was used to maintain optimum moisture for seed germination and seedling establishment. Two weeks after seed germination, the root systems of seedlings were carefully rinsed in distilled water to remove any adhering sand. Uniform seedlings were transferred to 25 L plastic tubs containing modified Johnson’s nutrient solution in a completely randomized design. The composition of the full-strength nutrient solution (pH 5.5) was 5.0 mM N, 3.5 mM K, 1.5 mM Ca, 0.5 mM Mg, 2.05 mM S, 50 µM Cl, 0.5 µM Mo, 25.0 µM B, 0.5 µM Cu, 2.0 µM Mn, 2.0 µM Zn, and 50.0 µM Fe as Fe-EDTA. The seedlings were held with foam plugs in the holes of a thermopore sheet placed at the top of each tub. Initially, plants grew for 20 days after transplanting (DAT) with adequate P supply (200 µM Pi). At 20 DAT, three replications of each cultivar were harvested, and the remaining plants were divided into two groups that received nutrient solution with either 200 µM Pi or without Pi and harvested at 30, 40 and 50 DAT. At each harvest, plant roots were rinsed (2–3 times) with distilled water to remove the nutrient solution. Shoot samples were washed with distilled water, blotted dry, and then separated into the stem, young (top) leaves and mature (lower) leaves. Young and mature leaves were further dissected from the middle into leaf tip (upper half) and leaf base (lower half). Plant material was dried at 70 °C for 48 h in a forced air-driven oven and stored under desiccation until weighing for dry biomass.

Experiment 2

Experimental site, design and crop management

Two rice cultivars contrasting in P utilization efficiency were selected from Experiment 1 [Super Basmati (P-inefficient) and PS-2 (P-efficient)] and grown in plastic pots containing 7 kg of soil. Bulk soil from the top 15 cm was collected from the research area of the Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan. A composite sample of collected soil was subjected to various physicochemical properties. In brief, the soil was a clay loam, non-saline (EC_e, 2.12 dS m⁻¹), alkaline in soil reaction (pH_s, 7.76), low in Kjeldahl nitrogen (0.04%), organic matter (0.71%) and available phosphorus (2.01 mg kg⁻¹) and high in available potassium (160 mg kg⁻¹). The experiment was arranged in a completely randomized factorial design having three replicates. All pots were irrigated with canal water, applied from the top to soil saturation and kept for two days for soil settling. The required amounts of P and K were added at the time of pot filling while N was applied in three equal splits. Both rice cultivars were grown at two P levels [i.e., adequate (P addition at 50 mg kg⁻¹ soil) and deficient (native soil P at 5.30 mg kg⁻¹ without external P addition)]. Seeds of rice cultivars were sown in nursery trays containing similar soil to the pots. At the three-leaf stage, three uniform and healthy seedlings of each cultivar were transplanted to each pot. A water layer (1–2 cm) on the soil surface was maintained during the entire crop period. At maturity, plants were harvested and threshed manually to separate paddy from straw. After recording yield and related attributes, plant material was oven dried at 70 °C till further analysis.

Soil analysis

In order to determine physicochemical properties of soil used in experiment 2, soil sample was air-dried and grounded to pass through a 2 mm sieve. Soil texture was determined by performing mechanical analysis of soil separates (sand, silt, and clay) using hydrometer method by dispersing soil in sodium hexametaphosphate solution⁵⁷. Soil reaction of the saturated soil paste (pH_s) and electrical conductivity of saturation extract (EC_e) were determined with pH and EC meter, respectively⁵⁸. Soil available phosphorus and potassium were estimated using ammonium bicarbonate-diethylene triamine penta-acetic acid (AB-DTPA) as extracting solution⁵⁹. Kjeldahl nitrogen was determined following the method described by Jackson⁶⁰. Soil organic matter (SOM) was quantified by chromic acid digestion according to Walkley-Black method⁶¹.

Plant analysis

Oven-dried plant material from both experiments was finely ground in a sample grinder (IKA Werke, Wilmington, USA). Ground samples (0.3 g each) were wet digested using 10 mL of di-acid digestion mixture [(HNO₃:HClO₄ (5:1, v/v)]. Total P concentration in plant samples was determined following the vanadate–molybdate method⁶² using UV-visible spectrophotometer (Shimadzu UV-VIS 1201, Shimadzu Co. Kyoto, Japan).

Calculation methods

The following P-efficiency relations were calculated to establish the relationship between P levels and plant growth of rice cultivars, i.e., P uptake⁶³, internal P remobilization⁶⁴, acquisition efficiency², utilization efficiency⁶⁵, and stress factor⁶⁶ using the following formulas:

• Phosphorus uptake (PU)

  $$\mathrm{PU}(\mathrm{mg}{\mathrm{plant}}^{-1})=\mathrm{P}\mathrm{concentration}(\mathrm{mg}{\mathrm{g}}^{-1})\times \mathrm{dry}\text{matter}(g{\mathrm{plant}}^{-1})$$
• Internal phosphorus remobilization (IPR)

  $$\mathrm{IPR}(\%)=\frac{\mathrm{P}{\mathrm{uptake}}_{\mathrm{before}\mathrm{P}\mathrm{omission}}-\mathrm{P}{\mathrm{uptake}}_{\mathrm{after}\mathrm{P}\mathrm{omission}}}{\mathrm{P}{\mathrm{uptake}}_{\mathrm{before}\mathrm{P}\mathrm{omission}}}\times 100$$
• Phosphorus acquisition efficiency (PACE)

  $$\mathrm{PACE}(\%)=\frac{\mathrm{P}{\mathrm{uptake}}_{\mathrm{adequate}\mathrm{P}}-\mathrm{P}{\mathrm{uptake}}_{\mathrm{deficient}\mathrm{P}}}{\mathrm{Quantity}\mathrm{of}\mathrm{P}\mathrm{applied}}\times 100$$
• Phosphorus utilization efficiency (PUTE)

  $$\mathrm{PUTE}(\%)=\frac{\mathrm{Paddy}{\mathrm{yield}}_{\mathrm{deficient}\mathrm{P}}}{\mathrm{Paddy}{\mathrm{yield}}_{\mathrm{adequate}\mathrm{P}}}\times 100$$

• Phosphorus stress factor (PSF)

$$\mathrm{PSF}(\%)=\frac{\mathrm{Paddy}{\mathrm{yield}}_{\mathrm{adequate}\mathrm{P}}-\mathrm{Paddy}{\mathrm{yield}}_{\mathrm{deficient}\mathrm{P}}}{\mathrm{Paddy}{\mathrm{yield}}_{\mathrm{adequate}\mathrm{P}}}\times 100$$

Statistical analysis

The computer software STATISTIX 8.1 (Analytical Software, Inc., Tallahassee, FL, USA) was used to perform statistical analysis following the methods of Steel et al.⁶⁷. All data reported in this study are the means of three replicates and presented with standard errors. First experiment was conducted following completely randomized desing and the significant differences among rice cultivars regarding growth, biomass production and P accumulation in various plant tissues were differentiated at each P level using one-way analysis of variance (ANOVA) technique with an associated least significant difference test at 5% probability level (LSD, P ≤ 0.05). In second experiment, completely randomized design with factorial arrangement was employed and the results for yield, P uptake and P-efficiency relations were compared by two-way ANOVA technique with an associated LSD test at P ≤ 0.05. The graphical presentation of data was performed usign Microsoft Office (Redmond, WA, USA).

Author contributions

M.I., T.A. and M.A.M. planed the experiment, M.I. and H.M.B. conducted the experiment and analyze data, M.I. and T.A. wrote the paper and K.S. and M.X. helps in data analysis and paper writing.

Data availability

All data generated or analysed during this study are included in this published article.

Competing interests

The authors declare no competing interests.