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. 2013 Jul 19;15(1):43–48. doi: 10.1179/174329210X12650506623203

Kinetic change of oxidative stress in cerebrospinal fluid of mice infected with Angiostrongylus cantonensis

Li-Yu Chung 1, Lian-Chen Wang 2, Chun-Hsiang Chen 3, Hsiao-Yi Lin 1, Chuan-Min Yen 1
PMCID: PMC7067334  PMID: 20196928

Abstract

The cerebrospinal fluid (CSF) of C57BL/6 mice infected with Angiostrongylus cantonensis was examined for kinetic changes in oxidative stress parameters, including reactive oxygen species (ROS), superoxide dismutase (SOD), catalase, malondialdehyde (MDA), 8-isoprostane, and 8-hydroxy-2′-deoxyguanosine (8-OHdG). The ROS increased gradually in the early stage of infection. During days 12–30 post-infection, the infected mice revealed ROS levels significantly higher than that in uninfected controls (P < 0.001). The ROS levels peaked at day 24 and then returned to that observed in uninfected controls at day 45 post-infection. The kinetics of MDA, 8-isoprostane, and 8-OHdG concentration changes observed in the CSF of the infected mice corresponded with kinetic changes in ROS levels. Thus, the excess ROS caused lipid peroxidation and DNA damage to cells in the central nervous system (CNS) of mice infected with A. cantonensis despite the increased antioxidant SOD and catalase enzyme activities during post-infection days 12–30. The oxidative stress in the CNS of C57BL/6 mice was apparently increased by diseases associated with A. cantonensis infection.

Keywords: ANGIOSTRONGYLUS CANTONENSIS, REACTIVE OXYGEN SPECIES, SUPEROXIDE DISMUTASE, MALONDIALDEHYDE, 8-ISOPROSTANE, 8-OHDG, OXIDATIVE STRESS

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