FIG 2.

Microscopic analysis of filament formation in Candida auris tup1 mutant (UACa11 background). (A) Representative microscopic images of C. auris filaments after growing tup1Δ cells in YPD broth with or without the addition of the indicated drug for 18 to 20 h at 30°C (top, bright-field images; bottom, merged fluorescent images showing chitin stained by calcofluor white [blue] and DNA stained by SYBR green I [green]). Scale bar, 10 μm. (B) Length of filaments observed after growth of wild-type (UACa11) (n = 150) and tup1Δ (n = 50) cells in YPD broth containing 100 mM HU for 18 to 20 h at 30°C. Only cells longer than 6 μm were considered filaments. The dotted line indicates the average length of 300 yeast cells (wild-type UACa11 grown in YPD for 18 to 20 h at 30°C). ns, not significant (Wilcoxon rank sum test).