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. 2020 Feb 19;10(8):3779–3792. doi: 10.7150/thno.44115

Figure 2.

Figure 2

Fasting before or after wound injury facilitates the healing of burns. (A) Illustration of the experimental procedure for testing the effects of fasting-1 and fasting-2 regimens on burn wound healing. (B) Gross images of burn wounds from mice in different treatment groups at days 4, 6, 8 and 12 post-wounding. Scale bar: 2 mm. (C) The rates of wound closure rates. n = 10 per group. (D) H&E staining of burn wounds at days 12 post-wounding. Ep: epithelium. Scale bar: 500 μm. (E and F) The rates of re-epithelialization (E) and widths of scars (F). n = 5 per group. (G and H) Masson's trichrome staining images of burn wounds at days 12 post-wounding (G) and the mean intensities for Masson-positive areas (H). Scale bar: 500 μm (top) or 50 μm (bottom). n = 5 per group. (I and J) Ki67 staining images of burn wounds at days 12 post-wounding (I) and the numbers of ki67-positive skin cells (J). Scale bar: 50 μm. n = 5 per group. For (C): Two-way ANOVA combined with Bonferroni post hoc test. For (E, F, H and J): One-way ANOVA combined with Bonferroni post hoc test. For (C): *P < 0.05 vs. control group, #P < 0.05 vs. fasting-1 group. For (C, E, F, H and J): *P < 0.05, **P < 0.01, ***P < 0.001.