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. 2020 Feb 19;10(8):3779–3792. doi: 10.7150/thno.44115

Figure 5.

Figure 5

Fasting stimulates the expression of angiogenic genes in endothelial cells. (A and B) The differentially expressed genes (DEGs) in endothelial cells subjected to fasting alone for 24 h (F-24 h: F24), fasting for 24 h followed by refeeding for 24 h (F-24 h + N-24 h: F24N24), or non-fasting for 24 h (N-24 h: N24) or 48 h (N-48 h: N48) were illustrated as a heatmap (A) or three volcano plots (B). |log2(foldchange)| > 0.58 and adjusted p value (padj) < 0.05 were used as the cutoff to identify DEGs. (C-E) Heatmaps showing the expression of a class of pro-angiogenic genes in different groups. (F) The expression of pro-angiogenic genes of interest was shown as a column diagram. n = 3 per group. (G) qRT-PCR analysis of the expression of SMOC1 and SCG2. n = 3 per group. (H) Western blotting for SMOC1 and SCG2 in N24, F24, F24N24 and N48 groups. For (F and G): One-way ANOVA combined with Bonferroni post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001.