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. 2020 Feb 2;9(2):128. doi: 10.3390/antiox9020128

Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf

Mohamad Fawzi Mahomoodally 1,2, Kouadio Ibrahime Sinan 3, Kouadio Bene 4, Gokhan Zengin 3,*, Giustino Orlando 5,*, Luigi Menghini 5, Serena Veschi 5, Annalisa Chiavaroli 5, Lucia Recinella 5, Luigi Brunetti 5, Sheila Leone 5, Paola Angelini 6, Vit Hubka 7, Stefano Covino 6, Roberto Venanzoni 6, Marie Carene Nancy Picot-Allain 2, Laura De Lellis 5, Alessandro Cama 5,8, Zoltán Cziáky 9, József Jekő 9, Claudio Ferrante 5
PMCID: PMC7070247  PMID: 32024319

Abstract

Bridelia species have been used in traditional African medicine for the management of diverse human ailments. In the current work, the detailed phytochemical profiles of the extracts of the stem bark of B. speciosa were evaluated and the antioxidant and enzyme inhibitory properties of the extracts were assessed. The anti-bacterial and anti-mycotic effects of the extracts were evaluated against selected pathogen strains. Additionally, the anti-proliferative effects were studied on the liver cancer HepG2 cell line. Finally, the putative protective effects were assessed on isolated rat liver that was challenged with lipopolysaccharide (LPS). The results revealed the presence of 36 compounds in the ethyl acetate extract, 44 in the methanol extract, and 38 in the water extract. Overall, the methanol extract showed the highest antioxidant activity, particularly in LPS-stimulated rat liver. Additionally, this extract exerted the highest antimycotic effect on C. albicans, whereas the water extract showed a promising anti-proliferative effect on liver cancer HepG2 cells. The methanol extract was also the most active as enzyme inhibitor, against acetylcholinesterase and butyrylcholinesterase. The current study appraises the antioxidant and enzyme inhibition properties of B. speciosa methanol extract and showed that this specie could be a promising source of biologically active phytochemicals, with potential health uses.

Keywords: Bridelia speciosa, antioxidant, anti-proliferative, antimicrobial, phenolic compounds

1. Introduction

Plants have played a pivotal role in the progress of mankind, being considered as a substantial source of food and medicine. Traditionally used, for their curative properties among different populations of the world, medicinal plants are still considered to provide outstanding curative effects and they remain the most accessible therapeutic approach to a number of ailments. In traditional medicine, herbal remedies are prepared according to “standardized formula” transmitted from elders or shamans. Some of the preparation methods include decoction, infusion, maceration, tinctures, among others which can be administered by different routes, including optical, dermal, oral, nasal, and anal [1]. The WHO has publicized the need for documentation of ethnomedicinal data on plants, being conscious of the wealth of traditional knowledge that is related to medicinal use represents. Ethnomedicinal records make scientific validation easier and also provide rational regarding the use of plants/herbal preparations for the management of specific ailments [2]. Global public interest for plants-derived products has undoubtedly increased and, today, one of the challenges is to provide scientific evidences of claimed biological activity, but also to unlock the potential of underexplored medicinal plants.

Recently, several endeavors have been made to probe for new sources of bioactive compounds from natural raw materials [3,4]. Among them, the bark of plants is one of the most important sources of bioactive compounds, including phenolics, flavonoids, and terpenes. In addition, extracts that were prepared from barks have been reported to possess broad biological activities, such as antioxidant, antimicrobial, or anti-cancer [3,4,5,6,7]. Based on these data, new studies on uninvestigated bark samples, particularly from Africa, might lead to the discovery of novel bioactive compounds for potential uses in the nutraceutical and pharmaceutical industries.

The Bridelia genus consists of approximately 60–70 species distributed in tropical and subtropical regions of the globe, particularly in Asia and Africa [8]. Several species of this genus have been used in traditional medicinal systems for the management of multiple diseases, including diabetes, urinary stones, lumbago, rheumatism, venereal diseases, bronchitis, gastrointestinal problems, cardiac pain, infertility, epilepsy, and diarrhoea, among others [9]. Keeping this in view, the biological efficacy of several Bridelia species has been claimed in several research pieces [10,11,12,13,14,15]. In earlier studies, the chemical profiles of the members of the Bridelia genus have been reported. For example, previous studies have reported the presence of phenolic acids (gallic acid and ellagic acid, etc.), tannins, and flavonoids in several Bridelia species, including B. ferruginea, B. micrhanta, and B. retusa. Such studies also highlighted the importance of the Bridelia genus, which could open avenues for new studies [16,17,18].

As far as our literature search could ascertain, little scientific information was available on B. speciosa. In this perspective, the current work aims at characterizing the stem bark extracts of B. speciosa investigating phytocompounds and elucidating the antioxidant, enzyme inhibitory properties, protective and anti-proliferative effects in experimental models of liver cancer and inflammation.

2. Materials and Methods

2.1. Plant Material and Preparation of Extracts

The plant samples were collected from wild areas in Gontougo region (Nioumassi) of Ivory Coast in 2018 and they were identified by Dr. Kouadio Bene, botanist at the Laboratoire de Botanique et Phytothérapie, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire. A voucher specimen of the plant material was deposited at Selcuk University, Science Faculty (KIS-1005). The stem barks samples were randomly collected from ten plants in a same population. The stem barks samples were taken stripped vertically while using a knife to the limit of the cambium layer. The stem barks were separated and then dried at room temperature for ten days.

One laboratory mill (Retsch Cutting Mill SM 200, Haan, Germany) was used to powder them (about 2 mm). The extraction procedure was conducted following traditional maceration (for ethyl acetate and methanol) and infusion (for water) methods. Briefly, for maceration, 5 g powdered plant samples was stirred with solvents (100 mL) overnight at the room temperature. Subsequently, the solvents were evaporated using a rotary-evaporator. For water extracts, 5 g powdered plants in boiled water (100 mL) was allowed to stand for 20 min. The aqueous extract was then lyophilized and all of the extracts were kept in +4 °C until use.

2.2. Chemicals

The chemicals were purchased from Sigma–Aldrich (Darmstadt, Germany). They were: 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), gallic acid, rutin, caffeic acid, electric eel acetylcholinesterase (AChE) (type-VI-S, EC 3.1.1.7), horse serum butyrylcholinesterase (BChE) (EC 3.1.1.8), galantamine, acetylthiocholine iodide (ATChI), butyrylthiocholine chloride (BTChI) 5,5-dithio-bis(2-nitrobenzoic) acid (DTNB), tyrosinase (EC1.14.18.1, mushroom), glucosidase (EC. 3.2.1.20, from Saccharomyces cerevisiae), amylase (EC. 3.2.1.1, from porcine pancreas), sodium molybdate, sodium nitrate, sodium carbonate, Folin-Ciocalteu reagent, hydrochloric acid, sodium hydroxide, trolox, EDTA, neocuproine, cupric chloride, ammonium acetate, ferric chloride, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), ammonium molybdate, ferrozine, ferrous sulphate hexahydrate, kojic acid and acarbose. All of the chemicals were of analytical grade.

2.3. Phytochemical Composition

The total bioactive compounds were determined colorimetrically, as described previously [19,20,21]. The results were expressed as mg of standard compounds (gallic acid for phenolic; and rutin for flavonoids; caffeic acid for total phenolic acid; catechin for total flavanol and tannins; quillaja for saponins) per g of dried extract. The bioactive profile of the B. speciosa extracts was determined while using a Dionex Ultimate 3000RS UHPLC instrument. All of the analytical and chromatographic details are given in Supplemental Materials. B. speciosa water and methanol extracts (5 µg/mL) were also analyzed for accurate phenolic quantitative determination of epicatechin, catechin, and gallic acid while using a reversed phase HPLC-fluorimetric in gradient elution mode, as recently described [22]. The experimental details are given in Supplemental Materials.

2.4. Determination of Antioxidant and Enzyme Inhibitory Effects

For antioxidant capacity, different test systems, including radical quenching, reducing power, phosphomolybdenum, and ferrous ion chelating, were used. The methods details were described in our earlier paper [23]. Standard trolox and EDTA equivalents were selected as standards to explain results. For enzyme inhibitory effects, key enzymes for global health problems were selected, namely α-amylase and α-glucosidase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase. Similar to the antioxidant assays, standard equivalent method (acarbose for amylase and glucosidase; galatamine for AChE and BChE; kojic acid for tyrosinase) was selected [23]. Experimental details are given in the Supplemental Materials.

2.5. Antimicrobial and Antimycotic Assays

Antibiotic and antimycotic assays were performed according to our previous studies [24,25]. The detailed description is reported in the Supplementary Material.

2.6. Cell lines and Treatments

Human hepatocellular carcinoma HepG2 cells were cultured in DMEM (Sigma, St. Louis, MO, USA) that was supplemented with 10% FBS at 37 °C, 5% CO2. B. speciosa methanol and water extracts were solubilized in phosphate-buffered saline (PBS) by sonication and then centrifuged at 1200 rpm for five minutes to remove the insoluble fraction. Supernatants were filtered through 0.2 μm pore diameter filters (Euroclone).

2.7. Cell Viability Assay

Cell viability was tested by MTT assay [3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; Sigma, St. Louis, MO, USA]. Briefly, the HepG2 cells were seeded in 96-well plates (5 × 103 cells/well) and they were treated the following day for 24, 48, or 72 hours with B. speciosa methanol and water extracts at various concentrations as indicated, or with vehicle (control). The MTT assay was performed as previously described [26]. The IC50 values were calculated while using the CompuSyn software.

2.8. Ex Vivo Studies

Sprague–Dawley rats (200–250 g) were sacrificed by CO2 inhalation (100% CO2 at a flow rate of 20% of the chamber volume per min) and the liver specimens were immediately collected and then maintained at 37 °C for 4 h in RPMI that was supplemented with E. coli LPS (10 µg/mL). During the incubation period, liver specimens were treated with either the methanol and water extracts of B. speciosa (10–500 µg/mL). Afterwards, the tissues were homogenized in 50 mM perchloric acid solution for biochemical determinations, as following described. The liver dopamine (DA) and 3-hydroxy-kinurenine (3-HK) levels were analyzed through an HPLC apparatus (Jasco PU 2080-plus) coupled to electrochemical detection (ESA Coulochem III). All of the details were given in our earlier paper [27].

2.9. Statistical Analysis

The results are given as mean ± S.D. One-way analysis of variance (ANOVA) with post-hoc Tukey test was conducted to determine significant differences (in total bioactive compounds, antioxidant, and enzyme inhibitory assays) between the extracts. ANOVA coupled to Newman–Keuls post-hoc test was employed for statistical analysis of data in pharmacological in vitro and ex vivo assays. GraphPad Prism 5.01 software was used to perform all of the statistical analyses. The results were considered to be statistically significant at p < 0.05.

3. Results and Discussion

While the term “phenolic compounds” includes a broad group of molecules containing at least one phenol unit, different other subgroups, such as flavonoids, tannins, and phenolic acids, among others, can be defined based on their chemical structures [28]. In the current work, the content of major phytochemical groups from B. speciosa stem bark extracts was evaluated while using spectrophotometric methods and are summarized in Table 1. The best concentration of flavanols, tannins, and saponins was observed in the B. speciosa stem bark methanol extract, whereas the highest flavonoid content was obtained in ethyl acetate extract. Flavonoids encompass a group of secondary metabolites having a distinct polyphenolic structure that consists of 15-carbon skeleton (two phenyl rings and one heterocyclic ring) [29]. On the other hand, flavanols or flavan-3-ols, which represent a popular group of flavonoids, include epicatechin and catechin, and their polymerization products [30]. Phenolic acids, which are the most widely distributed plant non-flavonoid phenolic compounds [31], carboxylic acids derivatives of either benzoic or cinnamic acid skeletons [32], were not identified from the ethyl acetate extract. However, HPLC-MS/MS was employed to more accurately evaluate the phytocomposition of B. speciosa stem bark extracts while considering the limitations of spectrophotometric determination in the assessment of phytocompound composition of herbal extract. With regards to the different extracts, 36 compounds were identified from the ethyl acetate extract, 44 from the methanol and 38 from the water extract of B. speciosa stem bark (Table 2). Ellagic, quinic, shikimic, gallic, and ferulic acids were characterized in all of the extracts, albeit the spectrophotometric determination did not reveal the presence of phenolic acids. Corilagin, an ellagitannin, having [M − H]− at m/z 633 was also characterized from all of the B. speciosa extracts. Mallotinic acid or its isomer, a hydrolyzable tannin [32] having [M − H]− at m/z 801, was identified from the methanol and water extracts. Bruguierol A ([M + H]+ at m/z 191), a dammarane triterpene [33] and Prodelphinidin B ([M − H]− at m/z 609), a polymeric tannin composed of gallocatechin, were characterized from the ethyl acetate and methanol extracts of B. speciosa stem bark. Tryptamine, which is a monoamine alkaloid, having [M − H]− at m/z 161, was identified from the methanol extract of B. speciosa stem bark only. Four unidentified tannins were identified from B. speciosa stem bark methanol extract.

Table 1.

Total bioactive components of the tested samples.

Samples Total phenolic Content (mg GAE/g Extract) Total Flavonoid Content (mg RE/g Extract) Total Phenolic Acid Content (mg CAE/g) Total Flavanol Content (mg CE/g) Total Tannin Content (mg CE/g) Total Saponin Content (mg QE/g)
EA 38.42 ± 0.38 c 5.85 ± 0.12 a nd 3.61 ± 0.02c 3.28 ± 0.38 c 177.82 ± 14.15 c
MeOH 224.28 ± 1.08 a 1.51 ± 0.04 b 11.55 ± 1.31 b 246.28 ± 10.63 a 324.09 ± 10.99 a 1031.45 ± 48.83 a
Water 210.29 ± 0.71 b 1.44 ± 0.17 b 13.91 ± 0.42 a 6.15 ± 0.18 b 67.83 ± 3.64 b 772.56 ± 56.39 b
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Values expressed are means ± S.D. of three parallel measurements. GAE: Gallic acid equivalent; RE: Rutin equivalent; CE: catechin equivalent; CAE: caffeic acid equivalent; QE: Quillaja equivalent; EA: Ethyl acetate; MeOH: Methanol; nd: not detected. Different letters indicate significant differences in the extracts (p < 0.05).

Table 2.

Chemical composition of the tested extracts.

No. Name Class 3 Formula Rt ± 0.03 min [M + H]+ [M − H]− Fragment 1 Fragment 2 Fragment 3 Fragment 4 Fragment 5 Detected in Extract 2
1 Quinic acid a C7H12O6 1.23 191.05557 173.0447 127.0388 111.0438 93.0331 85.0280 A,B,C
2 Shikimic acid a C7H10O5 1.31 173.04500 155.0338 137.0234 111.0439 93.0331 73.0280 A,B,C
3 Citric acid a C6H8O7 1.57 191.01918 173.0082 129.0181 111.0074 87.0073 85.0280 B,C
4 Prodelphinidin B b C30H26O14 1.73 609.12444 441.083 423.073 305.0672 177.0185 125.0231 B,C
5 1 Gallic acid (3,4,5-Trihydroxybenzoic acid) c C7H6O5 2.29 169.0137 125.0231 97.0282 81.0332 79.0175 69.0329 A,B,C
6 Gallocatechin (Casuarin, Gallocatechol) d C15H14O7 4.52 305.06613 261.0767 219.0651 167.0341 137.0234 125.0232 A,B,C
7 1 Tryptamine e C10H12N2 8.44 161.107875 144.0810 143.0732 117.0703 115.0546 103.0547 B,C
8 Syringic acid-4-O-glucoside f C15H20O10 10.57 359.09783 197.0451 182.0214 153.0546 138.031 123.0073 C
9 1 Catechin (Catechol, Catechuic acid) d C15H14O6 13.28 289.07121 245.082 203.0711 151.0389 125.0233 109.028 B,C
10 1 Epigallocatechin (Epigallocatechol) d C15H14O7 13.57 305.06613 261.0767 219.0658 167.0339 137.0234 125.0232 A,B,C
11 1 Vanillin (4-Hydroxy-3-methoxybenzaldehyde) g C8H8O3 15.47 153.05517 125.0601 111.0445 110.0367 93.0341 65.0393 A,B,C
12 1 Epigallocatechin-3-O-gallate (Teatannin II) d C22H18O11 16.39 457.07709 305.0661 169.0131 161.0238 125.0231 A,B,C
13 1 Gallocatechin-3-O-gallate d C22H18O11 16.40 457.07709 305.067 169.0133 161.0233 125.0231 C
14 Dihydrokaempferol-O-hexoside d C21H22O11 17.02 449.10839 287.0568 269.0447 259.0607 125.023 A,B,C
15 1 Epicatechin d C15H14O6 17.04 289.07121 245.0818 203.0706 151.0388 125.0231 109.028 B,C
16 3,5-Dimethoxy-4-hydroxybenzaldehyde (Syringaldehyde) g C9H10O4 17.24 183.06574 155.0705 140.0469 123.0444 105.0341 95.0498 A,B
17 Corilagin h C27H22O18 17.49 633.07279 463.0526 419.0627 300.9995 275.0205 169.0134 A,B,C
18 Mangiferin (Aphloiol, Chinonin) i C19H18O11 18.41 421.07709 343.0459 331.0464 301.0358 272.033 259.0249 A,B,C
19 Unidentified tannin 1 h C34H26O22 18.96 785.08375 633.0741 300.9992 275.0205 125.0229 B
20 Ferulic acid c C10H10O4 19.25 193.05009 178.0259 149.0594 137.023 134.0364 121.028 A,B,C
21 Mallotinic acid or isomer h C34H26O23 19.28 801.07867 757.0872 633.0753 613.047 463.0517 300.9995 B,C
22 1 Epicatechin-3-O-gallate d C22H18O10 19.37 441.08218 289.0725 271.0614 245.0808 169.0132 125.023 B,C
23 Loliolide j C11H16O3 19.47 197.11777 179.1071 161.0963 135.1172 133.1016 107.0861 A,B,C
24 Unidentified tannin 2 h C41H30O27 19.63 953.08963 300.9994 275.02 249.0387 B,C
25 Ellagic acid-4-O-glucoside k C20H16O13 19.90 463.05127 300.9995 299.9915 A,B,C
26 4-Hydroxy-3-methoxycinnamaldehyde (Coniferyl aldehyde) g C10H10O3 19.97 179.07082 161.0599 147.0443 133.0654 119.0496 55.0187 A,B,C
27 Unidentified tannin 3 h C34H26O22 20.08 785.08375 633.0734 300.9994 275.0207 B
28 Isoferulic acid c C10H10O4 20.30 193.05009 178.0264 149.06 137.0232 134.0362 121.0283 A
29 Unidentified tannin 4 h C34H26O22 21.25 785.08375 300.9996 275.0205 249.0402 125.0228 B,C
30 Myricitrin (Myricetin-3-O-rhamnoside) d C21H20O12 21.96 463.08765 317.0292 316.023 287.0213 271.0255 178.9978 B,C
31 Di-O-methylellagic acid-O-hexoside k C22H20O13 22.16 491.08257 476.0599 328.023 312.9996 297.9761 A,B,C
32 Ellagic acid-O-pentoside k C19H14O12 22.76 433.04071 300.9994 299.9916 283.9974 257.0082 A,B,C
33 Eschweilenol C (Ellagic acid-4-O-rhamnoside) k C20H16O12 23.09 447.05636 300.9994 299.9916 A,B,C
34 Pentahydroxyflavone-C-hexoside d C21H20O12 23.11 465.10331 447.0935 429.0806 369.0611 327.0503 303.0504 A
35 Ellagic acid k C14H6O8 23.38 300.99845 283.9967 257.0094 229.0138 201.0187 185.0237 A,B,C
36 Dimethoxy-trihydroxyflavone-O-hexoside d C23H24O12 24.29 491.11895 328.0586 313.0352 299.0195 285.0397 271.0252 B,C
37 Di-O-methylflavellagic acid O-hexoside k C21H18O13 24.70 507.07749 344.0187 328.994 313.97 A
38 Ducheside A (3-O-Methylellagic acid-4′-O-xyloside) k C20H16O12 24.74 447.05636 315.0151 314.0074 299.9917 298.983 270.9886 A,B,C
39 3,3′-Di-O-methylellagic acid-O-pentoside k C21H18O12 25.32 461.07201 446.0498 328.0228 312.9995 297.9757 A,B,C
40 3,3′,4-Tri-O-methylflavellagic acid-4-O-glucoside k C23H22O14 25.55 521.09314 506.0705 491.0473 358.0327 343.0098 327.9864 A,B,C
41 Eschweilenol A or isomer k C20H10O11 25.90 425.01449 300.9993 299.9917 298.9837 B
42 Dihydroactinidiolide j C11H16O2 26.58 181.12286 163.112 145.1015 135.1172 121.1015 107.0861 A,B,C
43 Di-O-methylellagic acid acetylhexoside k C24H22O14 27.49 533.09313 328.0231 312.9999 297.9756 269.9827 A
44 3,3′-Di-O-methylellagic acid k C16H10O8 27.84 329.02975 314.0073 298.9837 270.9887 A,B,C
45 Sebacic acid a C10H18O4 27.96 201.11268 183.102 157.1229 139.1117 111.0801 A
46 3,3′,4-Tri-O-methylellagic acid k C17H12O8 30.18 343.0454 328.0231 312.9995 297.9758 285.0038 A
47 Undecanedioic acid a C11H20O4 30.85 215.12834 153.1273 125.0956 A
48 3,3′,4-Tri-O-methylflavellagic acid k C17H12O9 31.21 359.04031 344.0171 328.9948 313.9717 300.9995 A,B,C
49 3,3′,4,4′-Tetra-O-methylellagic acid k C18H14O8 32.00 359.0767 344.0533 343.0448 329.0295 313.0347 A,B,C
50 Dihydroxy-trimethoxyflavone d C18H16O7 33.10 343.08178 328.0585 313.0359 298.0118 B
51 Bruguierol A l C12H14O2 36.06 191.10721 173.0965 161.0966 147.0801 135.0807 107.0496 A,B
521 Linoleic acid a C18H32O2 45.69 279.23241 261.2231 59.0124 A,B
53 Pheophytin A m C55H74N4O5 62.94 871.57375 593.277 533.2559 460.2264 A,B
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1 Confirmed by standard. 2 A: Ethyl acetate extract; B: Methanol extract; C: water extract. 3 a: carboxylic acid; b: polyflavonoid; c: phenolic acid; d: flavonoid; e: alkaloid; f: phenolic acid glucoside; g: phenolic aldehyde; h: tannin; i: xanthon; j: benzofuran; k: benzopyrane; l: phenolic heterocycle; m: porphyrin.

In the current work, radical scavenging, reducing power, and metal chelating assays were used to assess the antioxidant properties of B. speciosa stem bark extracts. This approach is believed to provide more accurate and comprehensive information regarding the antioxidant potential of herbal extracts [34]. The methanol extract was the most active in phosphomolybdenum assay, followed by the water and the ethyl acetate extracts (Table 3). A similar order was also obtained for total phenolic content (methanol extract >water extract >ethyl acetate extract). A positive correlation between concentration of phenols and antioxidant capacity was claimed in other studies, which suggests that high phenolic content could be an index of antioxidant capacity [35,36]. Deleterious effects of free radicals that are mainly caused by their instability and high reactivity lead to lipid, protein, and DNA alterations, thereby triggering diseases [37]. In line with the phosphomolybdenum assay, the methanol extract of B. speciosa stem bark was the most effective scavenger of DPPH (495.45 mg TE/g extract) and ABTS (902.33 mg TE/g extract). Apart from radical scavenging, the electron-donating capacity, as measured in terms of reducing power, is also regarded as an important antioxidant mechanism [38]. The CUPRAC and FRAP are the most common methods for measuring reducing power, in vitro. The FRAP evaluates the ability of the herbal extract to reduce ferric to ferrous, whereas the CUPRAC assay measures the conversion of cupric to cuprous [39]. Likewise, B. speciosa stem bark methanol extract showed the highest reducing activity (1325.89 and 952.68 mg TE/g extract, for CUPRAC and FRAP, respectively). However, the methanol extract (12.98 mg EDTAE/g) was the least active metal chelator. Indeed, transition metals, such as iron, can participate in Fenton reaction, converting hydrogen peroxide that is produced from mitochondrial oxidative respiration, into highly toxic hydroxyl free radical [40]. In the current study, the ethyl acetate extract, rich in flavonoids, showed the highest metal chelating properties (Table 3).

Table 3.

Antioxidant activities of the tested samples.

Samples Phosphomolybdenum
(mmol TE/g)		 DPPH
(mg TE/g Extract)		 ABTS
(mg TE/g Extract)		 CUPRAC
(mg TE/g Extract)		 FRAP
(mg TE/g Extract)		 Metal Chelating Ability (mg EDTAE/g)
EA 2.24 ± 0.07 c 18.62 ± 0.39 c 14.82 ± 0.45 c 94.34 ± 0.82 c 46.13 ± 0.58 c 32.08 ± 1.60 a
MeOH 5.89 ± 0.37 a 495.45 ± 0.53 a 902.33 ± 2.41 a 1325.89 ± 30.05 a 952.68 ± 23.61 a 12.98 ± 0.10 b
Water 5.17 ± 0.14 b 463.86 ± 14.04 b 581.14 ± 33.94 b 1082.42 ± 3.72 b 850.05 ± 5.35 b 14.28 ± 2.15 b
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Values expressed are means ± S.D. of three parallel measurements. TE: Trolox equivalent; EDTAE: EDTA equivalent; EA: Ethyl acetate; MeOH: Methanol. Different letters indicate significant differences in the extracts (p < 0.05).

Most of the therapeutic drugs that are clinically available function by inhibiting a specific enzyme [41]. Today, the challenge is to find novel inhibitors that can effectively correct metabolic imbalances, without causing side effects. The ability of B. speciosa stem bark extracts to inhibit cholinesterases, tyrosinase, α-amylase, and α-glucosidase was assessed and is reported in Table 4. The inhibition of cholinesterases, namely acetylcholinesterase and butyrylcholinesterase, remains the main focus for the management of Alzheimer’s disease. Besides, an increasing number of publications and clinical studies substantiates that Alzheimer’s disease and type 2 diabetes might share some pathophysiological similarities [42,43]. As such, type 2 diabetes has been identified as a risk factor for Alzheimer’s disease based on multiple connections [44]. From Table 4, it was noted that B. speciosa stem bark methanol extract exhibited the highest inhibition against acetylcholinesterase (4.98 mg GALAE/g extract) and butyrylcholinesterase (5.14 mg GALAE/g extract). The extracts showed a relatively low inhibition against α-amylase (ranging from 0.59 to 1.20 mmol ACAE/g extract), whereas only the ethyl acetate extract (3.56 mmol ACAE/g extract) actively inhibited α-glucosidase. Tyrosinase inhibition is the main therapeutic strategy for the management of epidermal hyperpigmentation conditions. The methanol extract (157.25 mg KAE/g extract) of B. speciosa stem bark showed highest inhibitory activity against tyrosinase. The observed enzyme inhibitory activity of B. speciosa stem bark methanol extract might be related to more than one phytochemical that is present in the extracts. Interestingly, molecular docking studies have previously shown that mangiferin effectively binds to acetylcholinesterase and butyrylcholinesterase [45]. Ferulic acid was reported to hybridise with quinoline in a competitive manner. Carbazole was reported to be more potent than galantamine, which showed pronounced inhibition against cholinesterases [46,47]. On the other hand, it was also reported that corilagin (IC50 = 1.231 mM) was active against tyrosinase [48]. Mangiferin was reported to exert a non-competitive inhibition of α-glucosidase [49].

Table 4.

Enzyme inhibitory properties of the tested extracts.

Samples AChE
(mg GALAE/g Extract)		 BChE
(mg GALAE/g Extract)		 Tyrosinase
(mg KAE/g Extract)		 α-Amylase
(mmol ACAE/g Extract)		 α-Glucosidase (mmol ACAE/g Extract)
EA 4.56 ± 0.20 b 3.59 ± 0.05 b 119.80 ± 1.30 c 0.86 ± 0.03 b 3.56 ± 0.03
MeOH 4.98 ± 0.04 a 5.14 ± 0.08 a 157.25 ± 0.48 a 1.20 ± 0.01 a na
Water 3.60 ± 0.15 c 2.61 ± 0.31 c 137.49 ± 0.35 b 0.59 ± 0.04 c na
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Values expressed are means ± S.D. of three parallel measurements. AChE: acetylcholinesterase; BChE: butyrylcholinesterase; GALAE: Galantamine equivalent; KAE: Kojic acid equivalent; ACAE: Acarbose equivalent; na: not active; EA: Ethyl acetate; MeOH: Methanol. Different letters indicate significant differences in the extracts (p < 0.05).

The methanol and water extracts were selected for further biological assays based on the results of colorimetric analyses indicating a more promising phytochemical profile in terms of total phenols and antiradical activity. A microbiological study was carried out to investigate the potential anti-fungal and anti-bacterial effects of selected pathogen strains, which are fully described in Supplementary Materials. The anti-microbial effects of both water and methanol extracts were compared with reference drugs and presented in Table 5 and Table 6. The results clearly demonstrated that the extracts were less effective when compared to the reference drugs, namely the anti-mycotics fluconazole and griseofulvin and the anti-bacterial ciprofloxacin. Nevertheless, the methanol extract of B. speciosa displayed anti-mycotic activity on C. albicans (YEPGA 6379) that deserves further investigation. This inhibitory effect is consistent with its major content in total phenolic compounds [24,25], as confirmed by both colorimetric assays (Table 1) and independent HPLC-fluorimetric analysis (Table 7). While considering both the incidence of C. albicans opportunistic infections occurring in liver disorders [50] and the traditional use of Bridelia genus [9], a pharmacological investigation was subsequently performed to explore anti-proliferative effects against the liver cancer HepG2 cell line and protective effects on isolated rat liver specimens challenged with the LPS pro-inflammatory stimulus. The HepG2 cell viability was evaluated through the MTT test, which revealed a stimulatory effect induced by methanol extract, up to 48 hours following treatment (Figure 1). Conversely, the water extract of B. speciosa was able to reduce cell viability in the concentration range (300–500 µg/mL) throughout the 72 hour treatment (Figure 1), thus indicating significant anti-proliferative effects that are consistent with more than one speculation. On one side, the concentration-dependent anti-proliferative effects that were exerted by the water extract could be related to epicatechins [51]. This was, at least in part, confirmed by the mild anti-proliferative effect that was exerted by the sole epicatechin (100 µg/mL) (CTR: 100; Epicatechin: 87.88 ± 4.35). On the other hand, we cannot exclude that the stimulating effect induced by methanol extract depends on its major content in phenolic compounds (Table 1 and Table 7), with particular regards to gallic acid, which could also exert putative pro-oxidant effects [22,52], thus potentially contributing to the maintenance of a microenvironment favorable to the proliferation of a cancer cell line [24]. Protective effects following extract treatment were also evaluated in a toxicological model constituted by isolated liver specimens that were stimulated with LPS, which increased the 3-HK levels in the liver tissue (Figure 2). 3-HK is a kinurenine-3-monooxygenase (KMO)-deriving kinurenine metabolite that is able to induce oxidative stress in multiple tissues, including the brain and pancreas [53,54]. Additionally, increased KMO activity was described in rodent models of acute pancreatitis [53], despite that there is still a lack of scientific evidence about KMO activity and 3-HK level in inflamed liver. Conversely, liver dopamine (DA) levels were reduced in the same experimental condition (Figure 3). Besides its crucial role as neurotransmitter in the central nervous system, recent findings suggest anti-inflammatory and protective effects that are induced by DA administration in experimental models of acute pancreatitis and hepatitis [55,56]. Consistent with the observed antiradical activity, both of the extracts reduced the LPS-induced levels of 3-HK (Figure 2). On the other hand, the water extract was completely ineffective against the LPS-induced levels of DA, thus ruling out the involvement of DA in mediating extract anti-oxidant effects, in the liver. By contrast, the methanol extract displayed a significant stimulating effect on liver DA concentration (Figure 3). This protective effect is consistent with both the antioxidant effect that is exerted by the extract and with literature [22,27]. Overall, the present pharmacological assays suggest that B. speciosa could be considered as a source of natural compounds with potential application in liver inflammation and cancer.

Table 5.

Minimal inhibitory concentrations (MICs) of B. speciosa water and methanol extracts, fluconazole, and griseofulvin against clinical yeasts and dermatophytes.

MIC (µg mL−1) *
Fungal Strains Methanol Extract Water Extract Fluconazole Griseofulvin
Candida albicans (YEPGA 6183) 396.85 (250–500) 198.42 (125–250) 2 >8
Candida albicans (YEPGA 6379) 49.6 (31.25–62.5) 78.74 (62.5–125) 1 >8
Candida tropicalis (YEPGA 6184) 629.96 (500–1000) 396.85 (250–500) 4 >8
Candida parapsilosis (YEPGA 6551) 78.74 (62.5–125) 99.21 (62.5–125) 2 >8
Arthroderma crocatum (IHEM 5251) 157.49 (125–250) 78.74 (62.5–125) 8 >8
Arthroderma crocatum (CCF 5207) 99.21 (62.5–125) 78.74 (62.5–125) >16 >8
Arthroderma insingulare (CCF 5417) 157.49 (125–250) 39.37 (31.25–62.5) >16 >8
Arthroderma quadrifidum (CCF 5792) 198.42 (125–250) 78.74 (62.5–125) >16 >8
Trichophyton erinacei (CCF 5930) 314.98 (250–500) 157.49 (125–250) >16 0.25
Trichophyton interdigitale (CCF 4823) 99.21 (62.5–125) 49.61 (31.25–62.5) >16 1
Trichophyton rubrum (CCF 4879) 78.74 (62.5–125) 78.74 (62.5–125) 8 2
Trichophyton tonsurans (CCF 4834) 157.49 (125–250) 39.58 (31.25–62.5) 2 0.125
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* MIC values are reported as geometric means of three independent replicates (n = 3); MIC range concentrations are reported within brachets. CCF, Culture Collection of Fungi, Department of Botany, Charles University, Prague, Czech Republic; IHEM, Belgian Coordinated Collections of Micro-organisms (BCCM/IHEM), Brussels, Belgium; YEPGA, yeast extract-peptone-glucose agar.

Table 6.

Minimum inhibitory concentration (MIC) of B. speciosa extracts and ciprofloxacin towards selected bacterial strains.

MIC (µg mL−1) *
Bacterial Strains Methanol Extract Water Extract Ciprofloxacin
Escherichia coli (ATCC 10536) 396.85 (250–500) 629.96 (500–1000) <0.12
Pseudomonas aeruginosa (ATCC 15442) 629.96 (500–1000) 314.98 (250–500) 1.23 (1.95–0.98)
Salmonella typhimurium (clinical isolate) 793.70 (500–1000) 793.70 (500–1000) 0.40 (0.25–0.5)
Bacillus cereus (ATCC 12826) 198.42 (125–250) 157.49 (125–250) <0.12
Bacillus subtilis (environmental isolate) 314.98 (250–500) 793.70 (500–1000) 0.01 (0.125–0.062)
Staphylococcus aureus (ATCC 6538) 198.42 (125–250) 396.85 (250–500) 0.62 (0.98–0.49)
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* MIC values are reported as geometric means of three independent replicates (n = 3); MIC range concentrations are reported within brachets.

Table 7.

Gallic acid, catechin and epicatechin level (µg/g dry extract) in methanol and water extracts of B. speciosa.

Compounds Methanol Extract Water Extract
Gallic acid 7228.36 ± 650.55 870.28 ± 36.81
Catechin 20.84 ± 2.51 n.d.
Epicatechin 188.72 ± 11.32 142.71 ± 7.75
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n.d.—not determined.

Figure 1.

Figure 1

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B. speciosa methanol and water extracts affect cell viability in human hepatocellular carcinoma HepG2. Cell viability was assessed by MTT assay after incubation for 24, 48, or 72 h, with the extracts at various concentrations as indicated, or with vehicle (control). Data shown are the means + SD of two independent experiments with quadruplicate determinations. Statistical analyses were performed using GraphPad Prism version 5.01 software (San Diego, CA). Comparisons of mean values between control and each drug concentration were performed by an unpaired Student’s t-test. A p-value ≤ 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001).

Figure 2.

Figure 2

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Effects of B. speciosa methanol and water extracts on LPS-induced 3-HK level in isolated rat liver specimens. ANOVA, p < 0.0001; ** p < 0.01, *** p < 0.001 vs. LPS control group.

Figure 3.

Figure 3

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Effects of B. speciosa methanol and water extracts on LPS-induced DA level in isolated rat liver specimens. ANOVA, p < 0.001; * p < 0.05, ** p < 0.01 vs. LPS control group.

4. Conclusions

This is the first report regarding the biological and phytochemical profiles of B. speciosa stem bark extracts. In this respect, our findings can be considered as the first attempt to provide new scientific information on the Bridelia genus. Among the three extracts studied, the methanol extract showed antioxidant and inhibitory properties against enzymes that are related to Alzheimer’s disease and epidermal hyperpigmentation conditions. The antioxidant effects displayed by the methanol extract were also consistent with the observed protective effects in the liver and the anti-mycotic effect against the C. albicans (YEPGA 6379) strain. The protective effects on rat liver induced by methanol extract were also substantiated by the increased DA and reduced 3-HK levels. On the other hand, the water extract reduced the HepG2 cell viability, thus suggesting potential anti-proliferative effects. Several compounds identified and quantified in the B. speciosa stem bark water and methanol extracts, including gallic acid and catechins, might be responsible for the observed effects. Therefore, the isolation of compounds from the methanol and water extracts is required for the validation of the observed pharmacological investigations. To sum up, our findings suggest that B. speciosa barks may be a key bio-resource for the development of novel pharmaceuticals or cosmeceuticals. Further studies are strongly recommended for exploring more biological properties through in vivo animal studies.

Acknowledgments

The experiments were approved by Local Ethical Committee (University “G. d’Annunzio” of Chieti-Pescara) and Italian Health Ministry (Italian Health Ministry authorization N. F4738.N.XTQ, delivered on 11 November 2018).

Supplementary Materials

The following are available online at https://www.mdpi.com/2076-3921/9/2/128/s1. Supplementary Materials and Methods; CFF-strains.

Click here for additional data file. (85.4KB, zip)

Author Contributions

Conceptualization, C.F. and G.Z.; methodology, G.O.; L.M.; software, L.M.; validation,: C.F.; G.Z.; L.M.; G.O.; formal analysis, C.F.; G.Z.; investigation, K.I.S.; K.B.; S.L.; L.R.; A.C. (Annalisa Chiavaroli); S.V.; P.A.; V.H.; S.C.; R.V.; L.D.L.; A.C. (Alessandro Cama); M.C.N.P.-A.; Z.C.; J.J.; M.F.M.; resources, C.F.; G.O.; L.M.; data curation, C.F.; G.Z.; writing—original draft preparation, M.F.M.; writing—review and editing, C.F., G.Z.; G.O.; L.M.; visualization, L.B.; supervision, L.B.; project administration, C.F.; L.M.; G.O.; G.Z.; funding acquisition, C.F.; G.O.; L.M. All authors have read and agreed to the published version of the manuscript.

Funding

The study was supported by Italian Ministry of University funds (FAR 2019) granted to Prof. Claudio Ferrante. The contribution of V.H. was supported by the grant of the Czech Ministry of Health (AZV 17–31269A).

Conflicts of Interest

The authors declare no conflict of interest.

References

  • 1.Kebebew M., Dadi K., Mohammed E. Diversity, Knowledge and Use of Traditional Medicinal Plants in Guduru District, Horo Guduru Wollega Zone, Oromia Region of Ethiopia. J. Med. Plants Stud. 2017;364:364–371. [Google Scholar]
  • 2.Ahmad Khan M.S., Ahmad I. Chapter 1-Herbal Medicine: Current Trends and Future Prospects. In: Ahmad Khan M.S., Ahmad I., Chattopadhyay D., editors. New Look to Phytomedicine. Academic Press; Cambridge, MA, USA: 2019. pp. 3–13. [Google Scholar]
  • 3.Feng S., Cheng S., Yuan Z., Leitch M., Xu C.C. Valorization of bark for chemicals and materials: A review. Renew. Sustain. Energy Rev. 2013;26:560–578. doi: 10.1016/j.rser.2013.06.024. [DOI] [Google Scholar]
  • 4.Tanase C., Coșarcă S., Muntean D.-L. A Critical Review of Phenolic Compounds Extracted from the Bark of Woody Vascular Plants and Their Potential Biological Activity. Molecules. 2019;24:1182. doi: 10.3390/molecules24061182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Ferrante C., Recinella L., Ronci M., Orlando G., Di Simone S., Brunetti L., Chiavaroli A., Leone S., Politi M., Tirillini B., et al. Protective effects induced by alcoholic Phlomis fruticosa and Phlomis herba-venti extracts in isolated rat colon: Focus on antioxidant, anti-inflammatory, and antimicrobial activities in vitro. Phytother. Res. 2019;33:2387–2400. doi: 10.1002/ptr.6429. [DOI] [PubMed] [Google Scholar]
  • 6.Orlando G., Ferrante C., Zengin G., Sinan K.I., Bene K., Diuzheva A., Jekő J., Cziáky Z., Di Simone S., Recinella L. Qualitative Chemical Characterization and Multidirectional Biological Investigation of Leaves and Bark Extracts of Anogeissus leiocarpus (DC.) Guill. & Perr (Combretaceae) Antioxidants. 2019;8:343. doi: 10.3390/antiox8090343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Tanase C., Mocan A., Coșarcă S., Gavan A., Nicolescu A., Gheldiu A.-M., Vodnar D.C., Muntean D.-L., Crișan O. Biological and chemical insights of beech (Fagus sylvatica L.) bark: A source of bioactive compounds with functional properties. Antioxidants. 2019;8:417. doi: 10.3390/antiox8090417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Maroyi A. Ethnopharmacology and therapeutic value of Bridelia micrantha (Hochst.) Baill. In tropical Africa: A comprehensive review. Molecules. 2017;22:1493. doi: 10.3390/molecules22091493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Ngueyem T.A., Brusotti G., Caccialanza G., Finzi P.V. The genus Bridelia: A phytochemical and ethnopharmacological review. J. Ethnopharmacol. 2009;124:339–349. doi: 10.1016/j.jep.2009.05.019. [DOI] [PubMed] [Google Scholar]
  • 10.Afolabi O.B., Oloyede O.I., Agunbiade S.O. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. J. Integr. Med. 2018;16:192–198. doi: 10.1016/j.joim.2018.04.006. [DOI] [PubMed] [Google Scholar]
  • 11.Corallo A., Foungbé S., Davy M., Cohen Y. Cardiovascular pharmacology of aqueous extract of the leaves of Bridelia atroviridis Muell. Arg. (Euphorbiaceae) in the rat. J. Ethnopharmacol. 1997;57:189–196. doi: 10.1016/S0378-8741(97)00071-8. [DOI] [PubMed] [Google Scholar]
  • 12.Olajide O.A., Makinde J.M., Okpako D.T., Awe S.O. Studies on the anti-inflammatory and related pharmacological properties of the aqueous extract of Bridelia ferruginea stem bark. J. Ethnopharmacol. 2000;71:153–160. doi: 10.1016/S0378-8741(99)00201-9. [DOI] [PubMed] [Google Scholar]
  • 13.Ramesh N., Viswanathan M.B., Saraswathy A., Brindha P., Balakrishna K., Lakshmanaperumalsamy P. Antibacterial activity of luteoforol from Bridelia crenulata. Fitoterapia. 2001;72:409–411. doi: 10.1016/S0367-326X(00)00317-8. [DOI] [PubMed] [Google Scholar]
  • 14.Sokeng S., Rokeya B., Mostafa M., Nahar N., Mosihuzzaman M., Ali L., Kamtchouing P. Antihyperglycemic effect of Bridelia ndellensis ethanol extract and fractions in streptozotocin-induced diabetic rats. Afr. J. Tradit. Complement. Altern. Med. 2005;2:94–102. doi: 10.4314/ajtcam.v2i2.31107. [DOI] [Google Scholar]
  • 15.Tatiya A.U., Saluja A.K., Kalaskar M.G., Surana S.J., Patil P.H. Evaluation of analgesic and anti-inflammatory activity of Bridelia retusa (Spreng) bark. J. Tradit. Complement. Med. 2017;7:441–451. doi: 10.1016/j.jtcme.2016.12.009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Cimanga K., Ying L., De Bruyne T., Apers S., Cos P., Hermans N., Bakana P., Tona L., Kambu K., Kalenda D. Radical scavenging and xanthine oxidase inhibitory activity of phenolic compounds from Bridelia ferruginea stem bark. J. Pharm. Pharmacol. 2001;53:757–761. doi: 10.1211/0022357011775893. [DOI] [PubMed] [Google Scholar]
  • 17.Jayasinghe L., Kumarihamy B.M., Jayarathna K.H., Udishani N.W., Bandara B.M., Hara N., Fujimoto Y. Antifungal constituents of the stem bark of Bridelia retusa. Phytochemistry. 2003;62:637–641. doi: 10.1016/S0031-9422(02)00623-4. [DOI] [PubMed] [Google Scholar]
  • 18.Pegel K.H., Rogers C.B. Constituents of Bridelia micrantha. Phytochemistry. 1968;7:655–656. doi: 10.1016/S0031-9422(00)88243-6. [DOI] [Google Scholar]
  • 19.Uysal S., Aktumsek A. A phytochemical study on Potentilla anatolica: An endemic Turkish plant. Ind. Crop. Prod. 2015;76:1001–1007. doi: 10.1016/j.indcrop.2015.08.017. [DOI] [Google Scholar]
  • 20.Vladimir-Knezevic S., Blazekovic B., Stefan M.B., Alegro A., Koszegi T., Petrik J. Antioxidant activities and polyphenolic contents of three selected Micromeria species from Croatia. Molecules. 2011;16:1454–1470. doi: 10.3390/molecules16021454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Zengin G., Aktumsek A. Investigation of antioxidant potentials of solvent extracts from different anatomical parts of Asphodeline anatolica E. Tuzlaci: An endemic plant to Turkey. Afr. J. Tradit. Complement. Altern. Med. 2014;11:481–488. doi: 10.4314/ajtcam.v11i2.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Di Giacomo V., Ferrante C., Ronci M., Cataldi A., Di Valerio V., Rapino M., Recinella L., Chiavaroli A., Leone S., Vladimir-Knežević S. Multiple pharmacological and toxicological investigations on Tanacetum parthenium and Salix alba extracts: Focus on potential application as anti-migraine agents. Food Chem. Toxicol. 2019;133:110783. doi: 10.1016/j.fct.2019.110783. [DOI] [PubMed] [Google Scholar]
  • 23.Grochowski D.M., Uysal S., Aktumsek A., Granica S., Zengin G., Ceylan R., Locatelli M., Tomczyk M. In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca. Phytochem. Lett. 2017;20:365–372. doi: 10.1016/j.phytol.2017.03.005. [DOI] [Google Scholar]
  • 24.Zengin G., Ferrante C., Gnapi D.E., Sinan K.I., Orlando G., Recinella L., Diuzheva A., Jekő J., Cziáky Z., Chiavaroli A. Comprehensive approaches on the chemical constituents and pharmacological properties of flowers and leaves of American basil (Ocimum americanum L.) Food Res. Int. 2019;125:108610. doi: 10.1016/j.foodres.2019.108610. [DOI] [PubMed] [Google Scholar]
  • 25.Zengin G., Ferrante C., Orlando G., Zheleva-Dimitrova D., Gevrenova R., Recinella L., Chiavaroli A., Leone S., Brunetti L., Aumeeruddy M.Z. Chemical profiling and pharmaco-toxicological activity of Origanum sipyleum extracts: Exploring for novel sources for potential therapeutic agents. J. Food Biochem. 2019;43:e13003. doi: 10.1111/jfbc.13003. [DOI] [PubMed] [Google Scholar]
  • 26.Veschi S., De Lellis L., Florio R., Lanuti P., Massucci A., Tinari N., De Tursi M., di Sebastiano P., Marchisio M., Natoli C. Effects of repurposed drug candidates nitroxoline and nelfinavir as single agents or in combination with erlotinib in pancreatic cancer cells. J. Exp. Clin. Cancer Res. 2018;37:236. doi: 10.1186/s13046-018-0904-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Ferrante C., Recinella L., Locatelli M., Guglielmi P., Secci D., Leporini L., Chiavaroli A., Leone S., Martinotti S., Brunetti L. Protective effects induced by microwave-assisted aqueous Harpagophytum extract on rat cortex synaptosomes challenged with amyloid β-peptide. Phytother. Res. 2017;31:1257–1264. doi: 10.1002/ptr.5850. [DOI] [PubMed] [Google Scholar]
  • 28.Gan R.-Y., Chan C.-L., Yang Q.-Q., Li H.-B., Zhang D., Ge Y.-Y., Gunaratne A., Ge J., Corke H. 9-Bioactive compounds and beneficial functions of sprouted grains. In: Feng H., Nemzer B., DeVries J.W., editors. Sprouted Grains. Elsevier; Amsterdam, The Netherlands: 2019. pp. 191–246. [Google Scholar]
  • 29.Panche A.N., Diwan A.D., Chandra S.R. Flavonoids: An overview. J. Nutr. Sci. 2016;5:e47. doi: 10.1017/jns.2016.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Prasain J.K., Barnes S., Michael Wyss J. Chapter 24—Analyzing Ingredients in Dietary Supplements and Their Metabolites. In: Watson R.R., Preedy V.R., Zibadi S., editors. Polyphenols: Mechanisms of Action in Human Health and Disease. 2nd ed. Academic Press; Cambridge, MA, USA: 2018. pp. 337–346. [Google Scholar]
  • 31.Chandrasekara A. Phenolic Acids. In: Melton L., Shahidi F., Varelis P., editors. Encyclopedia of Food Chemistry. Academic Press; Oxford, UK: 2019. pp. 535–545. [Google Scholar]
  • 32.Menon R., Gonzalez T., Ferruzzi M., Jackson E., Winderl D., Watson J. Chapter One-Oats—From Farm to Fork. In: Henry J., editor. Advances in Food and Nutrition Research. Volume 77. Academic Press; Cambridge, MA, USA: 2016. pp. 1–55. [DOI] [PubMed] [Google Scholar]
  • 33.Nabeelah Bibi S., Fawzi M.M., Gokhan Z., Rajesh J., Nadeem N., Kannan R.R., RDDG A., Pandian S.K. Ethnopharmacology, Phytochemistry, and Global Distribution of Mangroves—A Comprehensive Review. Mar. Drugs. 2019;17:231. doi: 10.3390/md17040231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Mahomoodally M.F., Mollica A., Stefanucci A., Aumeeruddy M.Z., Poorneeka R., Zengin G. Volatile components, pharmacological profile, and computational studies of essential oil from Aegle marmelos (Bael) leaves: A functional approach. Ind. Crop. Prod. 2018;126:13–21. doi: 10.1016/j.indcrop.2018.09.054. [DOI] [Google Scholar]
  • 35.McDonald S., Prenzler P.D., Antolovich M., Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem. 2001;73:73–84. doi: 10.1016/S0308-8146(00)00288-0. [DOI] [Google Scholar]
  • 36.Reddy N.S., Navanesan S., Sinniah S.K., Wahab N.A., Sim K.S. Phenolic content, antioxidant effect and cytotoxic activity of Leea indica leaves. BMC Complement. Altern. Med. 2012;12:128. doi: 10.1186/1472-6882-12-128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Lobo V., Patil A., Phatak A., Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacol. Rev. 2010;4:118–126. doi: 10.4103/0973-7847.70902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Baloglu M.C., Llorent-Martínez E.J., Aumeeruddy M.Z., Mahomoodally M.F., Altunoglu Y.C., Ustaoglu B., Ocal M., Gürel S., Bene K., Sinan K.I., et al. Multidirectional insights on Chrysophyllum perpulchrum leaves and stem bark extracts: HPLC-ESI-MSn profiles, antioxidant, enzyme inhibitory, antimicrobial and cytotoxic properties. Ind. Crop. Prod. 2019;134:33–42. doi: 10.1016/j.indcrop.2019.03.066. [DOI] [Google Scholar]
  • 39.Mahomoodally M.F., Picot-Allain C., Hosenally M., Ugurlu A., Mollica A., Stefanucci A., Llorent-Martínez E.J., Baloglu M.C., Zengin G. Multi-targeted potential of Pittosporum senacia Putt.: HPLC-ESI-MSn analysis, in silico docking, DNA protection, antimicrobial, enzyme inhibition, anti-cancer and apoptotic activity. Comput. Biol. Chem. 2019;83:107114. doi: 10.1016/j.compbiolchem.2019.107114. [DOI] [PubMed] [Google Scholar]
  • 40.Kıvrak E.G., Yurt K.K., Kaplan A.A., Alkan I., Altun G. Effects of electromagnetic fields exposure on the antioxidant defense system. J. Microsc. Ultrastruct. 2017;5:167–176. doi: 10.1016/j.jmau.2017.07.003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Bhagavan N.V., Ha C.-E. Chapter 6-Enzymes and Enzyme Regulation. In: Bhagavan N.V., Ha C.-E., editors. Essentials of Medical Biochemistry. Academic Press; San Diego, CA, USA: 2011. pp. 47–58. [Google Scholar]
  • 42.Klimova B., Kuca K., Maresova P. Global View on Alzheimer’s Disease and Diabetes Mellitus: Threats, Risks and Treatment Alzheimer’s Disease and Diabetes Mellitus. Curr. Alzheimer Res. 2018;15:1277–1282. doi: 10.2174/1567205015666180925110222. [DOI] [PubMed] [Google Scholar]
  • 43.Lee H.J., Seo H.I., Cha H.Y., Yang Y.J., Kwon S.H., Yang S.J. Diabetes and Alzheimer’s Disease: Mechanisms and Nutritional Aspects. Clin. Nutr. Res. 2018;7:229–240. doi: 10.7762/cnr.2018.7.4.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 44.Femminella G.D., Frangou E., Love S.B., Busza G., Holmes C., Ritchie C., Lawrence R., McFarlane B., Tadros G., Ridha B.H., et al. Evaluating the effects of the novel GLP-1 analogue liraglutide in Alzheimer’s disease: Study protocol for a randomised controlled trial (ELAD study) Trials. 2019;20:191. doi: 10.1186/s13063-019-3259-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Picot M.C., Zengin G., Mollica A., Stefanucci A., Carradori S., Mahomoodally M. In vitro and in silico studies of mangiferin from Aphloia theiformis on key enzymes linked to diabetes type 2 and associated complications. Med. Chem. 2017;13:633–640. doi: 10.2174/1573406413666170307163929. [DOI] [PubMed] [Google Scholar]
  • 46.Fang L., Chen M., Liu Z., Fang X., Gou S., Chen L. Ferulic acid—Carbazole hybrid compounds: Combination of cholinesterase inhibition, antioxidant and neuroprotection as multifunctional anti-Alzheimer agents. Bioorgan. Med. Chem. 2016;24:886–893. doi: 10.1016/j.bmc.2016.01.010. [DOI] [PubMed] [Google Scholar]
  • 47.Mo J., Yang H., Chen T., Li Q., Lin H., Feng F., Liu W., Qu W., Guo Q., Chi H., et al. Design, synthesis, biological evaluation, and molecular modeling studies of quinoline-ferulic acid hybrids as cholinesterase inhibitors. Bioorgan. Chem. 2019;93:103310. doi: 10.1016/j.bioorg.2019.103310. [DOI] [PubMed] [Google Scholar]
  • 48.Li N., Lin Z., Chen W., Zheng Y., Ming Y., Zheng Z., Huang W., Chen L., Xiao J., Lin H. Corilagin from longan seed: Identification, quantification, and synergistic cytotoxicity on SKOv3ip and hey cells with ginsenoside Rh2 and 5-fluorouracil. Food Chem. Toxicol. 2018;119:133–140. doi: 10.1016/j.fct.2018.05.018. [DOI] [PubMed] [Google Scholar]
  • 49.Shi Z.-L., Liu Y.-D., Yuan Y.-Y., Song D., Qi M.-F., Yang X.-J., Wang P., Li X.-Y., Shang J.-H., Yang Z.-X. In Vitro and In Vivo Effects of Norathyriol and Mangiferin on α-Glucosidase. Biochem. Res. Int. 2017;2017:1206015. doi: 10.1155/2017/1206015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 50.Chu H., Duan Y., Lang S., Jiang L., Wang Y., Llorente C., Liu J., Mogavero S., Bosques-Padilla F., Abraldes J.G. The Candida albicans exotoxin Candidalysin promotes alcohol-associated liver disease. J. Hepatol. 2019 doi: 10.1016/j.jhep.2019.09.029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 51.Khiewkamrop P., Phunsomboon P., Richert L., Pekthong D., Srisawang P. Epistructured catechins, EGCG and EC facilitate apoptosis induction through targeting de novo lipogenesis pathway in HepG2 cells. Cancer Cell Int. 2018;18:46. doi: 10.1186/s12935-018-0539-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 52.Abnosi M.H., Yari S. The toxic effect of gallic acid on biochemical factors, viability and proliferation of rat bone marrow mesenchymal stem cells was compensated by boric acid. J. Trace Elem. Med. Biol. 2018;48:246–253. doi: 10.1016/j.jtemb.2018.04.016. [DOI] [PubMed] [Google Scholar]
  • 53.Mole D., McFerran N., Collett G., O’Neill C., Diamond T., Garden O., Kylanpaa L., Repo H., Deitch E. Tryptophan catabolites in mesenteric lymph may contribute to pancreatitis-associated organ failure. Br. J. Surg. Inc. Eur. J. Surg. Swiss Surg. 2008;95:855–867. doi: 10.1002/bjs.6112. [DOI] [PubMed] [Google Scholar]
  • 54.Nakagami Y., Saito H., Katsuki H. 3-Hydroxykynurenine toxicity on the rat striatum in vivo. Jpn. J. Pharmacol. 1996;71:183–186. doi: 10.1254/jjp.71.183. [DOI] [PubMed] [Google Scholar]
  • 55.Han X., Li B., Ye X., Mulatibieke T., Wu J., Dai J., Wu D., Ni J., Zhang R., Xue J. Dopamine D2 receptor signalling controls inflammation in acute pancreatitis via a PP2A-dependent Akt/NF-κB signalling pathway. Br. J. Pharmacol. 2017;174:4751–4770. doi: 10.1111/bph.14057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 56.Zhou H., Tang L., Yang Y., Lin L., Dai J., Ge P., Ai Q., Jiang R., Zhang L. Dopamine alleviated acute liver injury induced by lipopolysaccharide/d-galactosamine in mice. Int. Immunopharmacol. 2018;61:249–255. doi: 10.1016/j.intimp.2018.06.004. [DOI] [PubMed] [Google Scholar]

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