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. 2020 Feb 11;25(4):775. doi: 10.3390/molecules25040775

Table 1.

Calibration curve parameters, limits of detection (LOD), and lower limits of quantification (LLOQ) for the quantification of different adipokines and apolipoproteins in human serum.

Protein External Calibration Curve LOD LLOQ
Slope Intercept R2 Peptide (ng/mL) Protein (ng/mL) Peptide (ng/mL) Protein (ng/mL)
Adipsin 3.11×104 1.41×106 0.97 0.63 21.63 1.88 64.88
Adiponectin 3.22×104 −7.72×104 0.99 0.71 13.21 2.13 39.62
Asprosin 2.11×104 −4.50×106 0.96 6.02 85.00 18.05 255.00
Chemerin 9.71×104 −5.97×105 0.99 1.91 27.93 5.74 83.78
Leptin 9.71×104 −5.97×105 0.99 2.30 37.28 6.89 111.85
PAI-1 3.78×105 −1.29×106 0.99 0.34 22.53 1.03 67.59
Progranulin 7.53×104 2.08×105 0.99 0.50 31.77 1.51 95.32
RBP-4 1.27×105 −2.54×106 0.99 30.09 575.25 90.27 1725.75
Vaspin 1.20×105 −9.41×104 0.99 0.44 23.59 1.31 70.76
Apo-A1 7.32×103 −6.99×105 0.98 46.86 1025.93 140.57 3077.80
Apo-A2 5.08×104 3.12×105 0.99 1.58 18.63 4.74 55.88
Apo-A4 1.97×104 3.55×104 0.99 1.65 75.67 4.94 227.00
Apo-B100 1.62×104 8.44×103 0.99 1.91 859.34 5.72 2578.03
Apo-C1 2.69×103 −4.43×104 0.99 4.04 31.11 12.11 93.32
Apo-C2 6.02×104 5.98×105 0.99 1.73 18.81 5.20 56.42
Apo-C3 5.00×103 −1.19×105 0.99 20.03 180.87 60.08 542.60
Apo-C4 3.65×104 −7.26×104 0.99 1.80 24.26 5.39 72.77
Apo-D 4.03×104 2.40×105 0.99 2.06 35.46 6.18 106.38
Apo-E 1.30×104 −1.47×105 0.99 5.01 120.51 15.03 361.54