Table 3.
Analysed genes in qPCR experiments with Caco-2 cells (human), C. elegans and broilers (Gallus gallus) and the oligonucleotide sequences of primers used.
| Genes | Forward Primer Sequence (5’–3’) | Reverse Primer Sequence (5’–3’) | Accession No. |
|---|---|---|---|
| Human | |||
| ACTB [49] | GCGGGAAATCGTGCGTGACATT | GATGGAGTTGAAGGTAGTTTCGTG | NM_001101 |
| B2M | TGAAGCTGACAGCATTCG | CAGACACATAGCAATTCAGG | NM_004048 |
| CLDN1 [50] | AACGCGGGGCTGCAGCTGTTG | GATGTTGTCGCCGGCATA | NM_021101 |
| CLDN3 | CACGCGAGAAGAAGTACA | TCTGTCCCTTAGACGTAGT | NM_001306 |
| GAPDH | TGGTATCGTGGAAGGACTCA | CAGTGAGCTTCCCGTTCAG | NM_002046 |
| HPRT1 | GACCCCACGAAGTGTTGGAT | ACTGGCGATGTCAATAGGACTC | NM_000194 |
| HSPA1A | GTGGAGGAGTTCAAGAGAA | GGTGATGGACGTGTAGAA | NM_005345 |
| HSPB1 | CTGGATGTCAACCACTTCGC | TATTTCCGCGTGAAGCACC | NM_001540 |
| HSPD1 | GAAATTGCCAATGCTCACCG | CTTGACTGCCACAACCTGAA | NM_002156 |
| OCLN [51] | GGACTCTACGTGGATCAGTATTTG | AATAATCATGAACCCCAGTACAATG | NM_002538 |
| RPL5 | TGGGCCAGAATGTTGCAGAT | AGGGACATTTTGGGACGGTT | NM_000969 |
| RPLP0 | TCTACAACCCTGAAGTGC | AAGGTGTAATCCGTCTCC | NM_001002 |
| C. elegans | |||
| act-1 | TGTTCCCATCCATTGTC | GCTCATTGTAGAAGGTGTG | NM_073418 |
| ama-1 | CTCCGTCGTTGACTGTAT | ATACCCATTCCTCGTCTTC | NM_068122 |
| pmp-3 | ATACGAAGCCACGGATAG | CTGTGTCAATGTCGTGAAG | NM_001269679 |
| hsp-16.2 | GAGAGATATGGCTCTGATGG | TCTCCTTGGATTGATAGCG | NM_071106 |
| hsp-1 | GCACGGAAAGGTAGAAATC | CGAACTTGCGTCCAATAAG | NM_070667 |
| daf-16 | GAATGGATGGTCCAGAATG | GATTCCTTCCTGGCTTTG | NM_001026423 |
| G. gallus | |||
| ACTB | ATGAAGCCCAGAGCAAAAGA | GGGGTGTTGAAGGTCTCAAA | NM_205518 |
| PGK1 | GGATAAGGTGGATGTGAAGG | AGAACTTGTCAGGCATGG | NM_204985 |
| SDHA [52] | CAGGGATGTAGTGTCTCGT | GGGAATAGGCTCCTTAGTG | NM_001277398 |
| YWHAZ | AGAGTCGTCTCAAGTATCG | CAACCTCAGCCAAGTAAC | NM_001031343 |
| CLDN1 | GTCATGGTATGGCAACAG, | GGTGGGTAGGATGTTTCA | NM_001013611 |
| HSPA2 | GGCTGGAGAGAAGAATGT | GTGCTTACGCTTGAACTC | NM_001006685 |
| OCLN | GCAGATGTCCAGCGGTTAC | GGTCCCAGTAGATGTTGGC | NM_205128 |