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. 2020 Feb 7;12(2):427. doi: 10.3390/nu12020427

Figure 4.

Figure 4

Rg3 altered lipid metabolism without affecting white adipocyte marker gene expression, and the effects were reversed by AMPK inhibitor. (A) The mRNA levels of white adipocyte marker genes in differentiated 3T3-L1 cells treated with Rg3 (40 μM) in combination with compound C (CC) for 24 h. (B) The mRNA levels of lipogenic genes in differentiated 3T3-L1 cells treated with Rg3 (40 μM) in combination with compound C (CC) for 24 h. (C) Oil-Red-O staining showing the accumulation of lipid droplets in differentiated 3T3-L1 cells treated with Rg3 (40 μM) in combination with compound C (CC, 10 uM) for 24 h (left) and its quantitative graph (right) using Gen5 (Bio Tek, Winooski, VT, USA). Data represent means ± SEM for n = 3. Asterisks indicate significant differences between marked samples (one-way ANOVA; n.s.: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001).