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. 2020 Jan 31;12(2):373. doi: 10.3390/nu12020373

Figure 5.

Figure 5

Effect of synbiotic on hepatic vasculature. Mice were treated as described in Figure 1. Upon euthanasia, the liver was dissected and flash frozen for immunoblotting or fixed in formalin and paraffin embedded for immunohistochemical analysis, histology, or RNA was prepared and used for qRT-PCR analysis. The liver sections were probed for the following: (A,B) von Willebrand Factor (vWF; green); (C,D) CD31 (red); (E–G) Type IV Collagen (red); (H) Type I Collagen; (I) MMP9 mRNA and (J) TIMP1 mRNA expression in liver, and presented as fold change. All images were acquired using a 10× objective, and a selected area was cropped and enlarged. Images are representative of at least replicate images captured per mouse in 8 to 12 mice per treatment group. Immunofluorescent images were semi-quantified, and immunoblot band densities were analyzed using ImageJ software and normalized to HSC70. Values represent means ± SEM. * p < 0.05; ** p < 0.001. Pair-fed saline (PF-S); Ethanol-fed saline (EF-S); Ethanol-fed synbiotic (EF-Syn).