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. 2020 Feb 5;9(2):372. doi: 10.3390/cells9020372

Figure 2.

Figure 2

CDKI-73 induces homotypic vesicle fusion and reduces number of Rab11 vesicles at the plasma membrane. (a,b) Time-lapse confocal imaging of cross-sections through fat body cells showing Rab11-GFP endosomes. Representative images were from fat body tissues (CG-CAL4 > UAS-Rab11-GFP/+) treated either with PBS (a) or 500 nM CDKI-73 (b). The acquisition speed was set to 0.3 frames/second. Arrows depict fusing Rab11 endosomes. Arrowheads show Rab11 tubular structures. Scale bars: 2.5 μm. (c–g) Confocal micrographs of cross-sections through the fat body cells showing Rab11-GFP vesicles (green) in relation to the plasma membrane outlined by CellMask™ Deep Red (red). Representative images were from fat body tissues treated either with PBS (c,g) or CDKI-73 at 50 nM (d), 500 nM (e) and 1 μM (f). Fat body tissues were from the following genotypes: CG-CAL4 > UAS-Rab11-GFP/+ (c–f) and UAS-lystRNAi/+; CG-CAL4 > UAS-Rab11-GFP/+ (g). Arrows depict small ≤1 µm Rab11 vesicles at the plasma membrane. Scale bars: 5 μm. (h) Histogram showing comparative analysis of the number of small ≤1 µm Rab11 vesicles at the plasma membrane. One-way ANOVA and Tukey’s multiple comparison test showed significant differences between the means in designated groups (depicted by different letters on the bars, p < 0.0001). Data are represented as mean ± SEM.