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. 2020 Jan 29;9(2):319. doi: 10.3390/cells9020319

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Distinctly recovered mRNA in fibroblasts and endothelial cells after coculture with tumor cell-derived small extracellular vesicles (TEX). Endothelial cells (EC) and fibroblasts (Fb) were cocultured for 2 d–3 d with 30 µg/mL BSp73AS (AS)- or AS-Tspan8-TEX. mRNA were isolated and subjected to deep sequencing (DS). (A) Fb mRNA and (B) EC mRNA ≥2-fold up- or downregulated by coculture with AS-, AS-Tspan8- or both AS- and AS-Tspan8-TEX. Fb mRNA responds significantly more frequently to coculture with TEX than EC mRNA. Both respond more abundantly to AS-Tspan8- than AS-TEX. AS- and AS-Tspan8-TEX not differing significantly points towards TEX stimulating target cell autonomous programs.