Figure 1.

Experimental design. A unilateral 6-hydroxydopamine (6-OHDA) injection into the medial forebrain bundle (MFB) was performed to induce the rat Parkinson’s Disease (PD) model. Animal behavioral analysis through rotameter and staircase behavioral tests was performed to validate the model 3 weeks after 6-OHDA injections. Afterwards, human bone marrow mesenchymal stem cells (hBM-MSCs) secretome was (5 weeks after 6-OHDA injections) locally administrated into the substantia nigra (SN) and striatum (STR), respectively. Levodopa (LD), in turn, was given by oral gavage. 1 and 4 weeks after this treatment procedures, fine motor behavioral assessment (i.e., staircase) was performed.