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. 2020 Feb 7;9(2):385. doi: 10.3390/cells9020385

Figure 6.

Figure 6

Klotho suppresses matrilin-2-induced inflammatory responses in human AVICs via inhibition of the PKR-NF-κB cascade. Human AVICs were treated with recombinant Klotho (0.5 μg/mL) for 2 h or left untreated, followed by stimulation with matrilin-2. (A) Recombinant Klotho suppressed matrilin-2-induced PKR activation. (B) Recombinant Klotho attenuated soluble matrilin-2-induced NF-κB activation. Values are means ± SE. n = 3 separate experiment; * P < 0.05 vs. control; # P < 0.05 vs. the same time point of matrilin-2 alone. (C) Klotho inhibited matrilin-2-induced NF-κB nuclear translocation. Representative images of immunofluorescence staining show NF-κB (red) in human AVICs. Alexa 488-tagged WGA (green) was applied to outline plasma membrane. DAPI (blue) was applied for nuclear counterstaining. Original magnification, ×40 objective. (D) Klotho attenuated matrilin-2-induced ICAM-1 expression. (E) Klotho reduced MCP-1 and IL-6 production induced by matrilin-2. Values are means ± SE. n = 5 separate experiments using distinct cell isolates; * P < 0.05 vs. control; # P < 0.05 vs. matrilin-2 alone.