Figure 3.

Live-cell imaging of proliferating T cells. Cells were incubated with citrate-coated superparamagnetic iron oxide nanoparticles (SPION^Citrate) at an iron concentration of 75 µg/mL for 24 h. After purification, they were stimulated with CD3/CD28/CD2 activator mix and recombinant human interleukin-2. Live-cell imaging was performed every 24 h. The scale bar displays 500 µm. Abbreviations: SPIONs: superparamagnetic iron oxide nanoparticles, SPION^Citrate: citrate-coated superparamagnetic iron oxide nanoparticles.