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. 2020 Feb 10;9(2):402. doi: 10.3390/cells9020402

Table 1.

Primers used to amplify pig BimEL and BimEL mutation.

Name Primer Sequence
BimEL Forward gaattcatggcaaagcaaccttccgatg
Reverse ggatccgcaatgtaagggggagggagggt
BimEL-S65A Forward ccactggccccaccgaccGCCcctggcccctttgctacc
Reverse GGCggtcggtggggccagtgggccctgggggctgccttg
BimEL-T112A Forward aaatcaacacaaGCCccaagtcctccttgccaagcc
Reverse acttggGGCttgtgttgatttgtcacaactcatggg
BimEL-S55A65A73A S55A-Forward gaccgctgcccccaaggcGCCccccagggcccactg
S55A-Reverse GGCgccttgggggcagcggtccccttctccttccgg
S73A-Forward tggcccctttgctaccagaGCCccgcttttcatcttcgtg
S73A-Reverse GGCtctggtagcaaaggggccaggggcggtcggtgg

Mutation of BimEL-S65A and BimEL-T112A were based on pEGFP-N1-BimEL, mutation of BimEL-S55A65A73A (S55A, S65A, and S73A) was based on pEGFP-N1-BimEL-S65A.