Figure 4.

Effect of TGF-β1 on gene and protein expression of A-kinase anchoring proteins (AKAPs) in BEAS-2B cells. (A) Gene expressions of AKAP1, AKAP5, AKAP11, AKAP12, AKAP95, Ezrin, and Yotiao were studied by real-time quantitative PCR. (B) Representative western blotting images and quantification of Yotiao, Ezrin and AKAP95. (C) Immunofluorescence images of Yotiao after 3 ng/mL TGF-β1 stimulation for 24 h. Scale bar represents 100 µm. Data represent 5–7 independent experiments. Data are expressed as mean ± SEM, * p < 0.05; significant difference between indicated groups.