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Levels of proinflammatory cytokines. Klotho expression was upregulated with pDNA, cells were treated for 72 h with monensin sodium or 168 h with LPS and secretion of IL-2 (A), IL-7 (B), and IL-3 (C) was evaluated with ELISA method. Bars indicate SD, n = 3, ** p < 0.01, * p < 0.05, no indication – no statistical significance (one-way ANOVA and Dunnett’s a posteriori test).
