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The effect of tol-mitoFluo, mitoFluo, and C10-FL (0.5 and 1.5 µM each) on the membrane permeabilization in B. subtilis. The changes in the membrane permeabilization were monitored by measuring the fluorescence of TO-PRO-3 (1 µM) in PBS buffer. The channel former alamethicin was added at the end of each recording as a positive control (concentration, 10 µg/mL).
