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. 2020 Feb 14;9(2):443. doi: 10.3390/cells9020443

Figure 6.

Figure 6

ROS production and redox status in intact KI cells. (A) Mitochondrial ROS production was monitored as oxidation of the fluorescent H2-DCFDA probe in intact HEK293 (blue, n = 3), COX4i1/2 KO (orange, n = 3), COX4i1 KI (green, n = 9, three replicates of three clones each), and COX4i2 KI cells (magenta, n = 9, three replicates of three clones each). Data are plotted the mean ± S.D. (B) The NAD+/NADH ratio was calculated from parallel measurements of NAD+ and NADH contents in intact cells using NAD/NADH-Glo™ Assay (Promega). Data are plotted as the mean ± S.D. of HEK293 (blue, n = 3), COX4i1/2 KO (orange, n = 3), COX4i1 KI (green, n = 9, three replicates of three clones each), and COX4i2 KI cells (magenta, n = 9, three replicates of three clones each). * p < 0.05 marks a significant difference between COX4i1 and COX4i2 KI cells.