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. 2020 Feb 18;9(2):469. doi: 10.3390/cells9020469

Figure 1.

Figure 1

Disulfiram’s metabolite bis-diethyldithiocarbamate-copper complex (CuET) causes DNA damage preferentially in S/G2 cells deficient for BRCA1 or BRCA2 proteins. H1299 cells expressing (doxycycline-) DOX-inducible shBRCA1 (A) or shBRCA2 (B) were cultivated for at least three days in DOX-containing media and then treated with CuET (250 nM) for 5 h, and γH2AX intensity was analyzed by quantitative microscopy. (C) H1229 shBRCA1 cells or (D) H1299 shBRCA2 cells were treated as in (A) a γH2AX intensity was quantified with respect to cyclin A positivity defining S/G2 phase. (E) H1229 shBRCA2 cells pre-incubated with DOX were treated with disulfram (DSF) (500 nM), bathocuproinedisulfonic acid (BCDS) (50 μM), or their combination for 5 h and γH2AX intensity was quantified. Box plot represents 25–75 quartiles, median, and whiskers non-outlier range. Scale bars = 10 μm.